Alternative treatments for disc degeneration: Effects on matrix homeostasis

椎间盘退变的替代治疗：对基质稳态的影响

• 批准号: 8411092
• 负责人: Gwendolyn A Sowa
• 金额: $ 12.49万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8411092
• 关键词: 1 year old; Acids; Address; Affect; Age; Aging-Related Process; Alternative Therapies; Animal Model; Animals; Biochemical Pathway; Biological Assay; Biological Availability; Biological Models; Biomechanics; Cartilage; Catabolism; Cell Culture Techniques; Cells; Centers for Disease Control and Prevention (U.S.); Chondroitin; Clinical Research; Clinical Treatment; Collagen; Data; Development; Diet; Dinoprostone; Environment; Equilibrium; Extracellular Matrix; Future; Gene Expression; Gene Expression Profiling; Glucosamine; Glucosamine Sulfate; Goals; Height; Histology; Homeostasis; Human; In Vitro; Inflammation; Inflammatory; Instruction; Intervention; Intervertebral disc structure; Investigation; Laboratories; Lead; Low Back Pain; Magnetic Resonance Imaging; Measurement; Measures; Mechanical Stimulation; Mechanical Stress; Mechanics; Mediator of activation protein; Metabolism; Metalloproteases; Modeling; Molecular; Motion; Movement; Nitric Oxide; Nutritional; Omega-3 Fatty Acids; Operative Surgical Procedures; Oral; Oryctolagus cuniculus; Outcome; Pathway interactions; Patients; Performance; Pharmaceutical Preparations; Physiological; Process; Proteoglycan; Regulatory Pathway; Reporting; Research; Robot; Role; Staging; Stimulus; Stress; Structural Genes; System; Techniques; Testing; Tissues; Training; Translating; Translations; Treatment Protocols; Vertebral column; Water; Whole Organism; Work; Yoga; aggrecan; aggrecanase; alternative treatment; base; chronic back pain; cyclooxygenase 2; design; dietary supplements; experience; human NOS2A protein; in vivo; in vivo Model; indexing; indium arsenide; inhibitor/antagonist; intervertebral disk degeneration; kinematics; novel; nucleus pulposus; prevent; programs; protective effect; response; simulation; therapeutic target

Intervertebral disc degeneration is characterized by progressive loss of disc height related to loss of water content and proteoglycans. Key mechanisms involved in this process include inflammatory and mechanical stress. It is biologically plausible that CAM therapies, such as yoga or nutritional supplements, may exert their beneficial actions on the spine through these pathways. The fundamental goals of this research are to investigate the mechanisms behind the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on matrix balance in the intervertebral disc through use of both in vitro and in vivo systems. The immediate impact of this work is in demonstrating the biologic effects of mechanical loading and nutritional supplements on the intervertebral disc using cell culture and animal model systems. This will provide us with a powerful experimental platform with which we can test the effect of various additional loading paradigms as well as nutritional supplements on disc matrix homeostasis. Upon elucidation of the effected biochemical pathways, future studies will correlate the model systems to human spine kinematics relevant to CAM motion based therapies. This will result in the ability to translate these findings into more mechanistically directed clinical studies and rationally targeted therapeutics, which represents the long term goal of the candidate. This work and the expertise gained through the proposed training plan will serve as initial building blocks for a research program in which CAM questions can be addressed at the molecular, tissue, and whole organism level. RELEVANCE (See instructions): In a CDC report from 2004, low back pain was identified as the most common condition for which patients sought alternative treatments such as yoga and oral supplements. To utilize these interventions most effectively, it is critical to understand how these therapies are facilitating their effects. The goals of this research are to understand the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on the cartilage of the spine.

椎间盘退变的特征是椎间盘高度的逐渐丧失与水分的丧失有关

项目成果

Part 1: Dual-tuned proton/sodium magnetic resonance imaging of the lumbar spine in a rabbit model.

• DOI: 10.1097/brs.0b013e318259ee98
• 发表时间: 2012-08-15
• 期刊: Spine
• 影响因子: 3
• 作者: Moon CH;Kim JH;Jacobs L;Zhao T;Sowa G;Vo N;Kang J;Bae KT
• 通讯作者: Bae KT

Part 2: Quantitative proton T2 and sodium magnetic resonance imaging to assess intervertebral disc degeneration in a rabbit model.

• DOI: 10.1097/brs.0b013e3182583447
• 发表时间: 2012-08-15
• 期刊: Spine
• 影响因子: 3
• 作者: Moon CH;Jacobs L;Kim JH;Sowa G;Vo N;Kang J;Bae KT
• 通讯作者: Bae KT

NSAID use in intervertebral disc degeneration: what are the effects on matrix homeostasis in vivo?

NSAID 在椎间盘退变中的应用：对体内基质稳态有何影响？

• DOI: 10.1016/j.spinee.2017.04.012
• 发表时间: 2017
• 期刊: The spine journal : official journal of the North American Spine Society
• 作者: Vaudreuil,Nicholas;Kadow,Tiffany;Yurube,Takashi;Hartman,Robert;Ngo,Kevin;Dong,Qing;Pohl,Pedro;Coelho,JPaulo;Kang,James;Vo,Nam;Sowa,Gwendolyn
• 通讯作者: Sowa,Gwendolyn

Glucosamine supplementation demonstrates a negative effect on intervertebral disc matrix in an animal model of disc degeneration.

• DOI: 10.1097/brs.0b013e318286b31e
• 发表时间: 2013-05-20
• 期刊: Spine
• 影响因子: 3
• 作者: Jacobs L;Vo N;Coelho JP;Dong Q;Bechara B;Woods B;Hempen E;Hartman R;Preuss H;Balk J;Kang J;Sowa G
• 通讯作者: Sowa G

The effects of glucosamine sulfate on intervertebral disc annulus fibrosus cells in vitro.

硫酸氨基葡萄糖对体外椎间盘纤维环细胞的影响。

• DOI: 10.1016/j.spinee.2013.11.058
• 发表时间: 2015
• 期刊: The spine journal : official journal of the North American Spine Society
• 作者: Sowa,GwendolynA;Coelho,JPaulo;Jacobs,LloydineJ;Komperda,Kasey;Sherry,Nora;Vo,NamV;Preuss,HarryG;Balk,JudithL;Kang,JameD
• 通讯作者: Kang,JameD