Eye Movement Maps in Mouse Superior Colliculus

项目摘要/摘要 哺乳动物上丘(SC)是一种集视觉和其他功能于一体的皮质下结构 感官信息启动眼睛和头部的定向运动。供应链的一个基本特征 组织是感觉输入和运动输出的表示是按地形排列的 对齐了。虽然在理解视觉表征的发展方面取得了很大的进展，但在 至于SC，机动地图是如何形成并与视觉地图对齐的，目前尚不清楚。为了夺取 利用老鼠身上可用的遗传工具，研究人员建议对小鼠进行全面的 小鼠SC运动地图的组织和发育研究。首先，电气方面 微刺激将在SC的深层进行，以诱发眼跳样的快速眼动 老鼠。眼动图的地形组织将通过系统地改变 刺激SC内的部位，并确定诱发运动的幅度和方向。单机 还将在深层进行记录，以确定单个神经元如何编码眼球运动 方向和幅度，以及如何在鼠标SC中映射这样的移动场。第二，相同的 实验将在从出生起就没有视觉体验的小鼠身上进行，并在现有的转基因小鼠身上进行 在SC中改变了视觉地图的鼠标行。这些实验将确定视觉输入是否 为运动地图的开发和视觉运动调整提供指导性信号。总而言之，建议的 实验将提供第一个系统的小鼠SC深层的图谱，并开始研究 关于影响感觉运动发育的因素。这些研究将对以下方面产生重要影响 对人类眼动障碍和其他由以下原因引起的疾病的理解和潜在的治疗 神经元连接的错误连接。 6.

Project Summary/Abstract The mammalian superior colliculus (SC) is a subcortical structure that integrates visual and other sensory information to initiate orienting movements of the eyes and head. A fundamental feature of SC organization is that the representations of sensory inputs and motor outputs are topographically arranged and aligned. While great progress has been made in understanding the development of the visual representation in the SC, how the motor maps are formed and aligned with the visual map remains unknown. In order to take advantage of the available genetic tools in mice, the investigators propose to perform a comprehensive investigation of the organization and development of motor maps in the mouse SC. First, electrical microstimulation will be conducted in the deep layers of the SC to evoke saccade-like rapid eye movements in mice. The topographic organization of the eye movement map will be revealed by systematically varying the stimulation sites in the SC and determining the amplitude and direction of the evoked movements. Single-unit recording will also be performed in the deep layers to determine how individual neurons encode eye movement direction and amplitude, and how such movement fields are mapped in the mouse SC. Second, the same experiments will be performed in mice deprived of visual experience from birth and in an existing transgenic mouse line that have altered visual maps in the SC. These experiments will determine whether visual inputs provide an instructive signal for motor map development and visuomotor alignment. Together, the proposed experiments will provide the first systematic mapping of the deep layers of the mouse SC and initiate studies on factors influencing sensorimotor development. These studies will have important implications for the understanding and potential treatment of human eye movement disorders and other diseases that result from miswiring of neuronal connections. 6