Eye Movement Maps in Mouse Superior Colliculus

小鼠上丘眼动图

• 批准号: 8594491
• 负责人: Jianhua Cang
• 金额: $ 23.18万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8594491
• 关键词: Animals; Anterior; Biological Models; Birth; Clinical; Data; Development; Disease; Epilepsy; Eye Movements; Felis catus; Foundations; Future; Genetic; Goals; Head; Human; Individual; Investigation; Location; Maps; Mental Retardation; Motor; Motor output; Movement; Mus; Nervous system structure; Neurologic; Neurons; Ocular Motility Disorders; Penetration; Preparation; Primates; Research; Research Personnel; Retinal; Role; Saccades; Schizophrenia; Sensory; Signal Transduction; Site; Structure; Transgenic Mice; Visual; Visual system structure; Wild Type Mouse; autism spectrum disorder; awake; electrical microstimulation; experience; islet; motor control; public health relevance; rapid eye movement; receptive field; relating to nervous system; research study; retinotopic; sensorimotor system; superior colliculus Corpora quadrigemina; tool; vision development; visual map; visual motor; visual receptive field

Project Summary/Abstract The mammalian superior colliculus (SC) is a subcortical structure that integrates visual and other sensory information to initiate orienting movements of the eyes and head. A fundamental feature of SC organization is that the representations of sensory inputs and motor outputs are topographically arranged and aligned. While great progress has been made in understanding the development of the visual representation in the SC, how the motor maps are formed and aligned with the visual map remains unknown. In order to take advantage of the available genetic tools in mice, the investigators propose to perform a comprehensive investigation of the organization and development of motor maps in the mouse SC. First, electrical microstimulation will be conducted in the deep layers of the SC to evoke saccade-like rapid eye movements in mice. The topographic organization of the eye movement map will be revealed by systematically varying the stimulation sites in the SC and determining the amplitude and direction of the evoked movements. Single-unit recording will also be performed in the deep layers to determine how individual neurons encode eye movement direction and amplitude, and how such movement fields are mapped in the mouse SC. Second, the same experiments will be performed in mice deprived of visual experience from birth and in an existing transgenic mouse line that have altered visual maps in the SC. These experiments will determine whether visual inputs provide an instructive signal for motor map development and visuomotor alignment. Together, the proposed experiments will provide the first systematic mapping of the deep layers of the mouse SC and initiate studies on factors influencing sensorimotor development. These studies will have important implications for the understanding and potential treatment of human eye movement disorders and other diseases that result from miswiring of neuronal connections. 6

项目概要/摘要 哺乳动物的上丘（SC）是一个皮质下结构，整合了视觉和其他功能 启动眼睛和头部定向运动的感官信息。 SC的基本特征 组织是感觉输入和运动输出的表示按地形排列和 对齐。虽然在理解视觉表现的发展方面已经取得了很大进展 在 SC 中，运动图是如何形成并与视觉图对齐的仍然未知。为了采取 利用小鼠中可用的遗传工具，研究人员建议进行全面的研究 研究小鼠 SC 中运动图的组织和发展。一、电气 微刺激将在 SC 的深层进行，以引起类似眼跳的快速眼球运动 老鼠。眼动图的地形组织将通过系统地改变 SC 中的刺激位点并确定诱发运动的幅度和方向。单机 还将在深层进行记录，以确定单个神经元如何编码眼球运动 方向和幅度，以及如何在鼠标 SC 中映射此类运动场。二、同样 实验将在从出生起就被剥夺视觉经验的小鼠和现有的转基因小鼠中进行 改变 SC 视觉图的小鼠系。这些实验将确定视觉输入是否 为运动图开发和视觉运动对齐提供指导信号。共同提出的 实验将提供小鼠 SC 深层的第一个系统图谱并启动研究 影响感觉运动发育的因素。这些研究将对 对人类眼球运动障碍和其他疾病的理解和潜在治疗 神经元连接错误。 6