Cell/Molecular Biology Core

细胞/分子生物学核心提供了几个主要和试点项目 主要在评估新鲜（未固定）标本方面为其建议提供最先进的支持。的 细胞/分子生物学核心提供的具体服务包括 (a) DMA、RNA 提取、 和组织样本中的蛋白质，(b) 基因分型，实时定量 RT-PCR，(c) 蛋白质免疫印迹， (d) cRNA核糖核酸探针的克隆和合成，(e) 原位进行放射性和非放射性 杂交，(f) 磷光成像分析，(g) 基于 HPLC 的神经化学测定，以及 (h) 激光 显微解剖。除了方法学专业知识外，CORE 还为以下方面提供建议和协作： 细胞和分子生物学研究的设计、实施和分析。 CORE 的指导者是 博士的有效监督。米德尔顿和瓦拉诺。通过仔细的咨询和实验设计， DEARC Pi 将受益于最高水平质量控制的统一应用。此外，原始 细胞/分子生物学核心生成的数据将定期添加到中央 DEARC 中 数据库，以便任何研究人员都可以分析它们的具体效果以及与数据可能的相关性 从他们的研究中产生的。细胞/分子生物学核心人员将协助每个 Pi 在这方面也将与动物和神经解剖学核心密切合作，以确保 根据实验设计，每次测定都使用正确的组织样本。

The CELL/MOLECULAR BIOLOGY CORE provides several of the MAIN and PILOT PROJECTS with state-of-the-art support for their proposals primarily in assessments of fresh (unfixed) specimens. The specific services provided by the CELL/MOLECULAR BIOLOGY CORE include (a) extraction of DMA,RNA, and protein from tissue samples, (b) genotyping, real-time quantitative RT-PCR, (c) Western immunoblotting, (d) cloning and synthesis of cRNA riboprobes, (e) performing radioactive and non-radioactive in situ hybridization, (f) phosphorimaging analysis, (g) HPLC-based neurochemical assays, and (h) laser microdissection. In addition to the methodoligcal expertise, the CORE provides advice and collaboration for the design, implementation, and analyses of cell and molecular biology studies. The CORE is directed by the capable oversight of Drs. Middleton and Vallano. Through careful consultation and experimental design, DEARC Pis will benefit from uniform application of the highest level of quality control. Moreover, the raw data generated by the CELL/MOLECULAR BIOLOGY CORE will be routinely added into the central DEARC database, so that any investigator can analyze them for specific effects and possible correlations with data generated from their studies. The CELL/MOLECULAR BIOLOGY CORE personnel will assist each of the Pi's in this regard, and will also work closely with the ANIMAL and NEUROANATOMY CORES to ensure that the correct tissue samples are being utilized in each assay according to the experimental designs.