Cellular & Molecular Core - Developmental Exposure Alcohol Research Center

细胞/分子生物学核心提供了几个主要和试点项目， 主要在新鲜（未固定）标本的评估方面为他们的建议提供最先进的支持。的 细胞/分子生物学中心提供的具体服务包括（a）提取DNA，RNA， 和蛋白质，（B）基因分型，实时定量RT-PCR，（c）Western免疫印迹， (d)克隆和合成cRNA核糖核酸探针，（e）进行放射性和非放射性原位 杂交，（f）磷光成像分析，（g）基于HPLC的神经化学测定，和（h）激光 显微解剖除了方法学专业知识，CORE还为以下方面提供建议和合作： 细胞和分子生物学研究的设计，实施和分析。核心是由 米德尔顿医生和瓦拉诺医生的有力监督通过仔细的咨询和实验设计， DEARC Pis将受益于最高水平质量控制的统一应用。此外，生 由细胞/分子生物学核心产生的数据将被常规地添加到中央DEARC中 数据库，以便任何研究人员都可以分析它们的具体影响以及与数据的可能相关性 从他们的研究中产生的。细胞/分子生物学核心人员将协助 在这方面，Pi也将与动物和神经解剖学核心密切合作，以确保 根据实验设计，正确的组织样品被用于每个测定中。

The CELL/MOLECULAR BIOLOGY CORE provides several of the MAIN and PILOT PROJECTS with state-of-the-art support for their proposals primarily in assessments of fresh (unfixed) specimens. The specific services provided by the CELL/MOLECULAR BIOLOGY CORE include (a) extraction of DMA, RNA, and protein from tissue samples, (b) genotyping, real-time quantitative RT-PCR, (c) Western immunoblotting, (d) cloning and synthesis of cRNA riboprobes, (e) performing radioactive and non-radioactive in situ hybridization, (f) phosphorimaging analysis, (g) HPLC-based neurochemical assays, and (h) laser microdissection. In addition to the methodoligcal expertise, the CORE provides advice and collaboration for the design, implementation, and analyses of cell and molecular biology studies. The CORE is directed by the capable oversight of Drs. Middleton and Vallano. Through careful consultation and experimental design, DEARC Pis will benefit from uniform application of the highest level of quality control. Moreover, the raw data generated by the CELL/MOLECULAR BIOLOGY CORE will be routinely added into the central DEARC database, so that any investigator can analyze them for specific effects and possible correlations with data generated from their studies. The CELL/MOLECULAR BIOLOGY CORE personnel will assist each of the Pi's in this regard, and will also work closely with the ANIMAL and NEUROANATOMY CORES to ensure that the correct tissue samples are being utilized in each assay according to the experimental designs.