Yellow Fever/Dengue Virus Competence in Aedes aegypt aegypti/formosus in Senegal

塞内加尔埃及伊蚊/台湾伊蚊的黄热病/登革热病毒能力

• 批准号: 8471637
• 负责人: WILLIAM C. BLACK
• 金额: $ 33.61万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8471637
• 关键词: Abdomen; Address; Adult; Aedes; Africa; Africa South of the Sahara; African; Agreement; Area; Asians; Behavior; Burkina Faso; Caribbean region; Characteristics; Chest; Chromosome Mapping; Chromosome inversion; Chromosomes; Chromosomes, Human, Pair 1; Chromosomes, Human, Pair 2; Chromosomes, Human, Pair 3; Collection; Competence; Complementary DNA; Culicidae; Data; Dengue; Dengue Virus; Disease; Funding; Gambia; Genes; Genetic; Genetic Markers; Genetic Recombination; Genetic Vectors; Genome; Genome Mappings; Geographic Distribution; Geographic Locations; Ghana; Goals; Habitats; Human; Hybrids; Individual; Infection; Island; Isoenzymes; Jamaica; Knowledge; Licensing; Light; Literature; Location; Maps; Measures; Midgut; Mitochondria; Mitochondrial DNA; Mosquito Control; Nuclear; Paracentric Inversion; Parents; Pattern; Penetration; Poaceae; Population; Population Dynamics; Population Genetics; Predisposition; Prevalence; Publications; Publishing; Quantitative Trait Loci; Relative (related person); Research; Senegal; Serotyping; Single Nucleotide Polymorphism; Site; Solid; South America; Surveys; Testing; Time; Vaccines; Virus; Visit; Work; Yellow Fever; Yellow Fever Virus Infection; Yellow fever virus; base; conditioning; disorder control; egg; forest; insight; offspring; research study; trait; vector; vector control

Aedes aegypti sensu latu (s.l.) is the primary vector to humans of yellow fever virus (YFV) and the four serotypes of dengue virus (DENV1-4). Without a licensed vaccine for DENV, and despite the existence of a safe, effective YFV vaccine, control of these diseases still primarily relies on vector control. Our long term goal is to increase knowledge of local population dynamics to generate more focused, appropriate, and hopefully sustainable control strategies. There are two recognized subspecies of Ae. aegypti: the presumed ancestral form Ae. aegypti formosus (Aaf), a sylvan mosquito inhabiting forest habitats in sub-Saharan Africa; and Ae. aegypti aegypti (Aaa), found circumglobally in tropical and subtropical regions in close association with humans. We have been studying gene flow, vector competence and the distribution of Aaa and Aaf in Senegal. We present data that show a northwest-southeast cline in the abundance of the two subspecies. Northwest collections consist of only Aaa while collections in the southeast are all Aaf. The two subspecies were sympatric in six tall grass savanna and scrub sites in central Senegal. Consistent with the literature, competence for DENV2 is correlated with the distribution of the subspecies. Northwestern Aaa collections have a high disseminated infection rate (DIR) while southeast Aaf collections have a low DIR associated with a midgut escape barrier for DENV2. Curiously, our data and a recent publication suggest continuous gene flow across all geographic locations and subspecies in Senegal. This presents a paradox: How can the distribution of vector competence and subspecies be regional when gene flow is continuous? We present exciting data from a QTL mapping experiment gone wrong that demonstrate reduced recombination in Aaa/Aaf hybrids, consistent with an hypothesis that chromosomal inversions exist between the two subspecies. Inversions would allow for gene flow between subspecies while preserving associations in subspecies between morphological markers and vector competence. The possibility of inversions in Ae. aegypti s.l. has been discussed but never proven due to an inability to accurately resolve their polytene chromosomes. Our specific aims are to generate an intensive linkage map of Aaf to define presumptive inversions. Markers arising from this map will be used to define the geographic distribution of inversions in Senegal. We then propose to map Aaf QTL that condition susceptibility to DENV2 and YFV. We also plan to visit 50 additional sites in Senegal not covered in our initial survey to provide a more thorough coverage of vector competence and the prevalence of the subspecies.

埃及感蚊是黄热病病毒（YFV）向人类传播的主要媒介