DNA SEQUENCING (SHARED RESOURCE)

DNA 测序（共享资源）

• 批准号: 8740835
• 负责人: ROBERT K Moyzis
• 金额: $ 4.68万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-11 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8740835
• 关键词: Adopted; Area; Biomedical Research; Blood capillaries; Cancer Center; Cancer Center Support Grant; Communities; DNA; DNA Resequencing; DNA Sequence; DNA Sequence Analysis; DNA Sequencing Facility; DNA Sequencing Shared Resource; Data; Diagnostic; Drops; Equipment; Funding; Future; Generations; Gold; Grant; Half-Life; Human Genome Project; Human Resources; Internet; Laboratories; Learning; Malignant Neoplasms; Nucleotides; Policies; Process; Protocols documentation; Reaction; Reagent; Recombinant DNA; Research Personnel; Retrieval; Running; Sampling; Sequence Analysis; Sequencing Biochemistry; Services; Shipping; Ships; Solutions; Technology; Time; Training; Universities; University of California Irvine Cancer Center; anticancer research; base; capillary; cost; cost effective; experience; gene therapy; genetic variant; member; news; programs; tool; vector; web site

The Cancer Center DMA Sequencing Core operates as a recharge facility to provide access to leading edge automated DMA sequence analysis. Investigators conduct their own sequencing reactions which are then run on ABI3100 sequencers for $2.50/sample. All data entry and retrieval is conducted by direct internet access for Member convenience. This user/core "co-op" format was adopted to 1) maximize throughput for the majority of Cancer Center users, 2) supply a reduced cost service not commercially available, and 3) provide training for future researchers, a primary function of a University. Approximately 15,000-20,000 reactions a year, from over 190 different users/Pis are analyzed. The majority of users are Cancer Center Members.

癌症中心DMA测序核心作为一个充电设施，提供先进的自动DMA序列分析。研究人员进行自己的测序反应，然后在ABI 3100测序仪上运行，每个样品2.50美元。所有数据输入和检索均通过直接互联网访问进行，以方便会员。采用这种用户/核心“合作社”形式是为了1）最大化大多数癌症中心用户的吞吐量，2）提供商业上不可用的降低成本的服务，以及3）为未来的研究人员提供培训，这是大学的主要功能。每年分析来自190多个不同用户/PI的大约15，000 - 20，000个反应。大多数用户是癌症中心的成员。