DNA SEQUENCING (SHARED RESOURCE)

DNA 测序（共享资源）

• 批准号: 7944546
• 负责人: ROBERT K Moyzis
• 金额: $ 1.15万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-06 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944546
• 关键词: Accounting; Adopted; Area; Bacterial Genome; Blood capillaries; Cancer Center; Cancer Center Support Grant; Computer Analysis; Core Facility; DNA; DNA Resequencing; DNA Sequence; DNA Sequencing Facility; Data; Data Collection; Development; Equipment; Future; Gel; Gene Targeting; Genes; Genomics; Genotype; Goals; Image; Injection product; Internet; Length; Microarray Shared Resource; Preparation; Price; Protein Microchips; Protocols documentation; Reaction; Reading; Recycling; Research Personnel; Resources; Retrieval; Running; Sampling; Scanning; Sequence Analysis; Services; Solid; Technology; Training; Universities; University of California Irvine Cancer Center; capillary; checkup examination; cost; flexibility; gel electrophoresis; genome sequencing; instrument; instrumentation; meetings; member; peer

The Cancer Center DMA Sequencing Core operates as a recharge facility to provide access to leading edge automated DMA sequence analysis. Investigators conduct their own sequencing reactions which are then run on ABI3100 sequencers for $2.50/sample. All data entry and retrieval is conducted by direct internet access for Member convenience. This user/core "co-op" format was adopted to 1) maximize throughput for the majority of Cancer Center users, 2) supply a reduced cost service not commercially available, and 3) provide training for future researchers, a primary function of a University. Approximately 15,000-20,000 reactions a year, from over 190 different users/Pis are analyzed. The majority of users are Cancer Center Members.

癌症中心DMA测序核心作为充电设施运行， 自动DMA序列分析。研究人员进行自己的测序反应，然后 在ABI 3100测序仪上运行，2.50美元/样品。所有数据输入和检索都是通过直接互联网进行的 方便会员使用。采用这种用户/核心“合作”格式是为了：1）最大化吞吐量， 大多数癌症中心用户，2）提供商业上无法获得的低成本服务，以及3） 为未来的研究人员提供培训，这是大学的主要功能。约15，000 - 20，000 反应一年，从超过190个不同的用户/PI进行了分析。大多数用户是癌症中心 成员