CAP:Cationic DNA Liposome^Microbial Complexes as Broad Spectrum Antimicrobials

CAP：阳离子 DNA 脂质体^微生物复合物作为广谱抗菌剂

• 批准号: 8556063
• 负责人: Catharine Bosio
• 金额: $ 10.41万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8556063
• 关键词: Antibiotic Resistance; Antibiotics; Antigens; Bacteria; Breathing; Cells; Complex; DNA; Development; Disease; Dose; Effectiveness; Ethanol; Francisella; Francisella tularensis; Goals; Heating; Human; Immune response; Individual; Infection; Laboratories; Lipids; Liposomes; Mediating; Membrane; Nature; Peptide Hydrolases; Preparation; Proteins; Resistance; Route; Symptoms; Techniques; Testing; Therapeutic; Time; Tularemia; Virulent; antimicrobial; flexibility; microbial; mortality; prophylactic; protective efficacy; sugar; transmission process; vector

We first determined that the active component of MPF was heat stable and proteinase resistant. This strongly suggested that the component was either lipid or sugar in nature. We next optimized techniques to isolate lipids and sugars from F. tularensis for testing in our CLDC preparations. We confirmed, similarly to MPF, that in the absence of CLDC neither the lipid preparations nor the sugars were able to provoke protective immune responses directed against F. tularensis in host cells. We next combined the lipid preparations with CLDC to determine their efficacy against F. tularensis infection. However, as inorganic molecules the lipids are resuspended in ethanol. We found that ethanol had a profoundly negative effect on the retention of CLDC liposomes.

我们首先确定MPF的活性成分具有热稳定性和抗蛋白酶性。 这强烈表明该组分本质上是脂质或糖。 我们接下来优化了从F.在我们的CLDC制剂中进行测试。 我们证实，与MPF类似，在不存在CLDC的情况下，脂质制剂和糖都不能激发针对F的保护性免疫应答。宿主细胞中的土拉菌。 我们接下来将脂质制剂与CLDC组合以确定它们对F.土拉菌感染 然而，作为无机分子，脂质重悬于乙醇中。 我们发现乙醇对CLDC脂质体的保留具有深刻的负面影响。