Molecular Biology/Gene Expression Core

分子生物学/基因表达核心

• 批准号: 8527497
• 负责人: GARY D. WU
• 金额: $ 10.88万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8527497
• 关键词: Antibodies; Bioinformatics; Biostatistics Core; Cell Line; Clinical; Core Facility; Databases; Emerging Technologies; Equipment; Esophageal; Esophageal Tissue; Future; Gel; Gene Expression; Genes; Genetic Engineering; Goals; Health Insurance Portability and Accountability Act; Human; Image; Image Analysis; Informed Consent; Institutional Review Boards; Lentivirus Vector; Molecular Biology; Morphology; Mus; Online Systems; Patients; Plasmids; Proteins; Quality Control; RNA; Reagent; Real-Time Systems; Reporter; Sampling; Services; Tissue Banking; Tissue Banks; Tissues; Work; carcinogenesis; cost effectiveness; human tissue; instrument; programs; quality assurance; repository

Seeinstructions): The Molecular Biology/Gene Expression Core Facility will provide critical services to support the scientific goals of this Program Project: 1) Reagent Bank- contains specific plasmids, expression constructs, reporter constructs, antibodies, RNA samples, and retroviral/adenoviral/lentiviral vectors of relevance to the study of esophageal carcinogenesis. As a result, these reagents have been organized and centralized. In addition, we will continue to store newly available reagents; 2) Image Analysis and Gene Expression Quantification- multiple instruments are available, such as (but not limited to) Phosphorimaging, Gel and Chemidoc, Agilent Bioanalyzer and Odyssey Infrared Protein Imaging System, real-time quantitative PCR, and transcriptional analysis for Dynex and Orion Luminometers; 3) Gene Profiling Services (subsidized) with gene microarrays (Affymetrix platform) through the Penn microarray facility (Dr. Baldwin) and the Penn bioinformatics facility (Dr. Tobias integrated within the Biostatistics Core); 4) Human Tissue Bank with an esophageal tissue repository and annotated clinical tissue component; 5) Esophageal Cell Line Bank (murine and human) with primary, immortalized, transformed and newly genetically engineered esophageal cell lines for use by the Projects; 6) Esophageal database repository (web-based) for esophageal tissues, cell lines (nontransformed and transformed, organotypic cultures, microarrays, and common, shared reagents/equipment (from Cores C and D). The Molecular Biology/Gene Expression Core provides quality assurance, quality control, cost- effectiveness, timeliness and efficiency in its services. All components that pertain to human tissues are IRB approved with patient informed consent and HIPAA compliance, and carry patient de-identifiers. This Core works closely with all the other Cores, especially the Morphology Core C to avoid duplication and furnish coordinated approaches for the Projects. The Projects benefit tremendously from this Core in advancing their interrelated hypotheses and Specific Aims. In addition, this Core is dynamic in responding to evolving P01 needs, anticipating future P01 directions, and integrating emerging technologies. RELEVANCE (Seeinstructions):

参见说明）： 分子生物学/基因表达核心设施将提供关键服务来支持科学 该计划项目的目标：1) 试剂库 - 包含特定质粒、表达构建体、报告基因 与研究相关的构建体、抗体、RNA 样本和逆转录病毒/腺病毒/慢病毒载体 食管癌发生。这样一来，这些试剂就被组织起来并集中起来。此外， 我们将继续储存新可用的试剂； 2) 图像分析和基因表达定量- 多种仪器可供选择，例如（但不限于）Phosphorimaging、Gel 和 Chemidoc、Agilent 生物分析仪和 Odyssey 红外蛋白质成像系统、实时定量 PCR 和转录 Dynex 和 Orion 发光计的分析； 3) 使用基因微阵列的基因分析服务（有补贴） （Affymetrix 平台）通过宾夕法尼亚大学微阵列设施（鲍德温博士）和宾夕法尼亚大学生物信息学设施 （托比亚斯博士纳入生物统计学核心）； 4) 含有食管组织的人体组织库 存储库和注释的临床组织成分； 5) 食管细胞系库（小鼠和人类） 原代、永生化、转化和新基因工程食管细胞系，供 项目； 6) 食管组织、细胞系（未转化）的食管数据库存储库（基于网络） 和转化的器官型培养物、微阵列以及常见的共享试剂/设备（来自 Cores C 和 D）。分子生物学/基因表达核心提供质量保证、质量控制、成本- 其服务的有效性、及时性和效率。所有与人体组织有关的成分都是IRB 经患者知情同意和 HIPAA 合规性批准，并携带患者去识别信息。这个核心 与所有其他核心密切合作，尤其是形态核心 C，以避免重复和提供 项目的协调方法。这些项目在推进其项目方面从该核心中受益匪浅 相互关联的假设和具体目标。此外，该核心能够动态响应不断发展的 P01 需求，预测未来 P01 方向，并整合新兴技术。 相关性（参见说明）：