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Biochemisty, Pathology, and Imaging Core

生物化学、病理学和影像核心

基本信息

批准号：
8378285
负责人：
Ravichandran Ramasamy
金额：
$ 22.77万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2017-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8378285
关键词：
3-deoxyglucosone Acids Adopted Albumins Animals Antibodies Aorta Apoptosis Atherosclerosis Biochemical Biochemistry Biological Assay Biology Blood Vessels Buffers Cardiovascular Diseases Cells Critiques Cultured Cells Detection Diabetes Mellitus Diacetyl Disease Enzyme-Linked Immunosorbent Assay Epitopes Equipment Fibronectins Fluorescence Functional disorder Generations Glucose Glucose-6-Phosphate Glucosephosphates Glutathione Glutathione Disulfide Heart High Pressure Liquid Chromatography Homidium Bromide Hour Hydrogen Peroxide Image Imaging Techniques Immunoglobulin G Immunohistochemistry Incidence Incubated Individual Instruction Letters Link Lysine Manufacturer Name Measurement Measures Mediator of activation protein Messenger RNA Methods Mitochondria Modification Molecular Probes Monitor Mus Myocardial Infarction NADPH Oxidase Oryctolagus cuniculus Oxidative Stress Oxis Pathology Perchloric Acids Peripheral arterial disease Plant Roots Plasma Post-Translational Protein Processing Procedures Process Proteins Publications Publishing Pyruvaldehyde Reaction Reader Reading Recommendation Research Sampling Severities Signal Transduction Standardization Sterility Superoxides System Techniques Time Tissues Weight Western Blotting angiogenesis catalase diacetyldichlorofluorescein ethyl lysine experience fluorophore fructosyl-lysine hydroethidine in vivo methionine sulfoxide molecular imaging myocardial infarct sizing novel pentosidine programs receptor for advanced glycation endproducts response

项目摘要

PROJECT SUMMARY (See instructions): Core B, the Biochemistry, Pathology and Imaging Core will continue to serve all three Projects through all five years of the Program. The core is composed of three units: (1) The Biochemistry Unit of Core B will perform assays on tissue/plasma of mice and cultured cells in the individual projects for assessment of oxidative stress, AGEs and other biochemical mediators linked to RAGE and vascular dysfunction. (2) The Pathology Unit of Core B will perform pathological analysis of mouse tissues. This Unit will serve all 3 projects for standardization of immunohistochemistry and semiquantitative analyses. The Unit will perform analysis of atherosclerosis (aortas (atherosclerosis at the aortic root and en face assessment of aorta), angiogenesis (Project 2-3) and myocardial infarction size (Project 3). (3) The newly-formed Imaging Unit will perform novel imaging techniques using state-of-the-art new equipment for molecular imaging studies in atherosclerosis and apoptosis (Project 1) and angiogenesis (Project 2).

项目总结（见说明）：核心B，生物化学，病理学和成像核心将继续服务于所有三个项目通过所有五年的计划。核心由三个单元组成：（1）核心B的生物化学单位将对个别项目中的小鼠组织/血浆和培养细胞进行分析，以评估氧化应激、AGEs和其他与血管功能障碍和血管功能障碍有关的生化介质。(2)Core B的病理学部门将对小鼠组织进行病理学分析。该单位将为所有3个项目提供免疫组织化学和半定量标准化服务。分析。该单位将对动脉粥样硬化（动脉粥样硬化（主动脉根部动脉粥样硬化和主动脉正面评估）、血管生成（项目2-3）和心肌梗死面积（项目3）进行分析。 (3)新成立的影像组将采用先进的新设备，进行新的影像技术，用于动脉粥样硬化和细胞凋亡（项目1）和血管生成（项目2）的分子影像研究。