National Biomedical EPR Center

国家生物医学EPR中心

• 批准号: 8475982
• 负责人: JAMES S HYDE
• 金额: $ 163.94万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8475982
• 关键词: Advisory Committees; Biological; Biological Process; Biomedical Research; Chairperson; Characteristics; Classification; Collaborations; Communities; Databases; Detection; Development; Electron Spin Resonance Spectroscopy; Electrons; Electrostatics; Enhancement Technology; Fourier Transform; Frequencies; Funding; Grant; Institutes; Ions; Lead; Letters; Link; Mails; Measurement; Membrane Proteins; Metals; Methods; Modality; Molecular Structure; Motion; National Institute of Biomedical Imaging and Bioengineering; National Institute of General Medical Sciences; Paper; Phase; Physiologic pulse; Physiological; Property; Protein Dynamics; Research; Research Personnel; Research Project Grants; Resources; Services; Signal Transduction; Site; Spectrum Analysis; Spin Labels; Techniques; Technology; Temperature; Training; United States National Institutes of Health; base; design; falls; frontier; improved; innovation; instrument; instrumentation; interest; magnetic field; microwave electromagnetic radiation; millimeter; millisecond; molecular dynamics; new technology; nitroxyl; programs; public health relevance; research and development; research study; software development; technology development; time use

DESCRIPTION (provided by applicant): The dominant usage in NIH-funded grants is in the field of site-directed spin-labeling (SDSL) using nitroxide radical spin labels to probe molecular structure. The Technology Research and Development (TR&D) component of this application for continued support of the National Biomedical EPR Center, a P41 Research Resource, focuses on technology enhancement for SDSL applications of EPR. The TR&D component is tightly linked to the Collaborative component, with most collaborations falling in the SDSL classification. Applications involving metal ions are next among NlH-supported grants using EPR, and in this submission, special emphasis is placed in the Service component on making EPR capabilities available to this community. Training and Dissemination components are broadly based and vigorous. We introduced the technology of non-adiabatic rapid sweep (NARS) spectroscopy in the current funding period as a general purpose replacement for phase-sensitive detection (PSD) in EPR spectroscopy. NARS capabilities will be built at L-, X-, Q-, W-, and D-band. Extension to adiabatic rapid sweep (ARS) was demonstrated at W-band during the current funding period, establishing feasibility for Fourier transform EPR using ARS technology with arbitrary waveform generator (AWG) sweep, which is proposed. The intermediate "passage" range of magnetic field sweep will be used to probe very slow motions. Microwave frequency sweep will be developed at Q-, W-, and D-band. TR&D Project 1 focuses on development of the new technology at L,- X-, and Q-band; Project 3 carries the technology to W- and D-band; and Project 2 focuses on two L-band applications: distance determination at room temperature to 40 A and L-band electron-electron double resonance (ELDOR) for measurement of protein and membrane electrostatic potentials. The M = 0 line of perdeutero 14N spin labels is narrow and very nearly isotropic, making it an ideal probe for these two experiments. New resonators tailored for NARS detection are proposed. The ten collaborations are very tightly linked to the three TR&D projects. The strong support letter for this project from Prof. Wayne L. Hubbell, UCLA, who is also chairman of the External Advisory Committee for the Center, is notable. Collaborations demonstrate widespread interest in the SDSL community. The TR&D theme in this submission is non-adiabatic, adiabatic, and rapid passage technology development across a wide range of microwave frequencies for SDSL research. The NIH RePORTER database reveals 124 R0I research grants that use EPR. We serve the holders of these grants to enhance the nation's biomedical research. Progress in the current funding period includes 220 papers.

描述（由申请人提供）：NIH资助的赠款的主要用途是使用氮氧自由基自旋标记来探测分子结构的定点自旋标记（SDSL）领域。本申请的技术研究和开发（TR&D）部分用于继续支持国家生物医学EPR中心（P41研究资源），重点是EPR SDSL应用的技术增强。TR&D组件与协作组件紧密相连，大多数协作都属于SDSL分类。涉及金属离子的应用是下一个使用EPR的NIH支持的赠款，在这次提交中，特别强调的是在服务组件上使EPR能力提供给这个社区。培训和传播部分的基础广泛，充满活力。我们介绍了 在本供资期内，将继续使用非绝热快速扫描（NARS）光谱技术，作为EPR光谱技术中相敏探测（PSD）的通用替代品。NARS能力将在L、X、Q、W和D波段建立。在本供资期内，在W波段演示了绝热快速扫描的扩展，确定了拟议的利用绝热快速扫描技术和任意波形发生器扫描进行傅立叶变换EPR的可行性。磁场扫描的中间“通道”范围将用于探测非常缓慢的运动。将在Q、W和D波段开发微波频率扫描。TR&D项目1侧重于L、X和Q波段的新技术开发;项目3将该技术应用于W和D波段;项目2侧重于两个L波段应用：室温下40 A的距离测定和用于测量蛋白质和膜静电势的L波段电子-电子双共振（ELDOR）。全氘14 N自旋标记的M = 0线很窄，并且非常接近各向同性，使其成为这两个实验的理想探针。提出了一种适合于NARS探测的新型谐振腔。这十项合作与三个研发项目紧密相关。来自韦恩教授的支持信。哈贝尔，加州大学洛杉矶分校，谁也是该中心的外部咨询委员会主席，是值得注意的。合作表明了SDSL社区的广泛兴趣。本次提交的TR&D主题是SDSL研究中广泛微波频率范围内的非绝热、绝热和快速通道技术开发。NIH Reporter数据库揭示了124个使用EPR的R 0 I研究赠款。我们为这些赠款的持有人提供服务，以加强国家的生物医学研究。在本资助期内的进展包括220篇论文。