Thermogenetic Tools for Manipulating Neuronal Activity in Mammals

操纵哺乳动物神经元活动的热遗传学工具

• 批准号: 8461996
• 负责人: Paul Garrity
• 金额: $ 30.34万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8461996
• 关键词: Adoption; Agonist; Animals; Behavior; Brain; Brain region; Cations; Characteristics; Chemicals; Complement; Deep Brain Stimulation; Disease; Drosophila genus; Effectiveness; Epilepsy; Exhibits; Focused Ultrasound Therapy; Goals; Heating; Human; Implant; Insecta; Investigation; Ion Channel; Light; Link; Major Depressive Disorder; Mammals; Maps; Mental Depression; Mental disorders; Mus; Mutagenesis; Neurons; Obsessive-Compulsive Disorder; Parkinson Disease; Physiological; Physiology; Procedures; Property; Proteins; Research Personnel; Slice; Source; Stimulus; Structure; System; TRPA1 Channel; Techniques; Temperature; Testing; Therapeutic; Toxic effect; Variant; Work; base; desensitization; excitatory neuron; fly; inhibitory neuron; nervous system disorder; optogenetics; postsynaptic; receptor; response; tool

DESCRIPTION (provided by applicant): Manipulating the activity of identified neurons in brain circuits is essential for studying how they are organized and how they produce behavior. Manipulating circuit activity can also be used to treat neurological and psychiatric diseases including Parkinson's Disease, depression and epilepsy. Optogenetic tools have revolutionized our ability to manipulate neuronal activity. We propose to create a set of complementary thermogenetic tools. These have the potential to reliably provide stronger levels of activation and may be activated with less invasive stimulus delivery systems. At present, such tools are used exclusively in flies. We propose to modify these tools to optimize them for use in mammalian systems.

描述（由申请人提供）：操纵脑回路中已识别神经元的活动对于研究它们如何组织以及它们如何产生行为至关重要。操纵回路活动也可用于治疗神经和精神疾病，包括帕金森病、抑郁症和癫痫。光遗传学工具彻底改变了我们操纵神经元活动的能力。我们建议创建一套互补的热成因工具。这些具有可靠地提供更强水平的激活的潜力，并且可以用侵入性较小的刺激递送系统激活。目前，这种工具仅用于苍蝇。我们建议修改这些工具，以优化它们用于哺乳动物系统。