Bioanalytical CoreBioanalytical Core

项目摘要(见说明)：生物分析核心(核心C)通过为急性肾损伤(AKI)研究提供最先进的生物能量学、氧化应激分析和代谢物分析支持，为加州大学伯克利分校和加州大学伯克利分校的奥布莱恩中心社区以及扩展的研究基地提供服务。核心C提供全面的资源，包括氧化应激和细胞生物能量学的生物分析分析、AKI的生物标记物、翻译后修饰和小分子生化变化的优化方案和技术。这些服务包括咨询、实验设计培训、回收适合分析和化验性能的样品。提供的新服务包括确定线粒体生物能量学的独特技术、基于LC-MS的肌酐、F2-异前列腺素和柠檬酸环中间体的分析。正在开发最先进的NanoLC-MS方法，在临床样本和AKI动物模型中进行靶向和非靶向代谢组学和外切体分析。Core C已经成功地支持了肾脏研究界。自核心C项目成立以来，已经为涉及56个项目的48名首席调查人员进行了8,500多次化验。在48名调查人员中，44人是非核心人员。每年使用Core C的调查人员数量都在增加，每年的发表率也在增加。核心C还支持了7名试点和可行性补助金获得者的研究工作。这些综合努力目前已在23份经同行审查的出版物中得到认可。中心将参与对调查人员的教育和培训，提供实践经验和科学交流。2013-2017年的一个经常性特征将是为期4天的代谢组学培训年度研讨会(由NIGMS资助，作为NIH代谢组学共同基金计划的一部分)。最后，生物分析核心将与奥布莱恩中心领导层合作，确定研究人员的新生物分析需求，并为该中心实施这些需求。

PROJECT SUMMARY (See instructions): The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.