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Skin Proteomics Core

皮肤蛋白质组学核心

基本信息

批准号：
7677162
负责人：
STEPHEN BARNES
金额：
$ 12.17万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7677162
关键词：
Animal Model Arts Biological Blood Body Fluids Bulla Cancer Center Cells Chemicals Computer software Consultations Data Databases Dermatology Detection Development Digestion Disease Educational Activities Educational workshop Epigallocatechin Gallate Event Experimental Designs Faculty Fingerprint Fluorescent Dyes Funding Gel Hand Human Resources Image Image Analysis Immunoprecipitation Individual Ions Isoelectric Focusing Isotope Labeling Laboratories Lead Liquid substance Management Information Systems Mass Spectrum Analysis Membrane Proteins Methods Modification Monitor Operative Surgical Procedures Peptide Hydrolases Peptides Pharmaceutical Preparations Plasma Post-Translational Protein Processing Preparation Prevention Principal Investigator Process Protein Analysis Proteins Proteome Proteomics Protocols documentation Quality Control Reaction Reagent Research Research Personnel Research Training Resources Robotics Role Sampling Science Services Skin Skin Tissue Specific qualifier value Spottings Staining method Stains Subcellular Fractions Sweat Sweating Techniques Technology Time Tissues Training University of Alabama at Birmingham Cancer Center Update Urine base cyanine dye 5 experience gel electrophoresis grape seed extract improved instrumentation interstitial member programs protein complex research study skin disorder small molecule symposium tandem mass spectrometry treatment effect

项目摘要

The proteomics Core D of the UAB Skin Disease Research Center provides support to pilot/feasibility awardees, SDRC-associated investigators and other skin disease researchers. This support consists of consultations imvolving two experienced senior faculty with expertise in 2D-gel proteomics and protein mass spectrometry, hands-on support by a junior faculty member skilled in several proteomics/mass spectrometry methods used and applied to skin disease research, training in the techniques offered by the Core, access to the instrumentation, and seminars by outside experts in proteomic and mass spectrometry. The specific services available in the Core include (1) development of experimental design and statistical considerations for experiments that include proteomics and mass spectrometry components; (2) 2D-gel separation of proteins from tissues, cells, subcellular fractions and specific body fluids (plasma/serum, urine, sweat, tears, tissue interstitial and blister fluids) - this involves isoelectric focusing/SDS-PAGE or clear native gel electrophoresis and makes use of Cy2, Cy3 and Cy5 fluorescent dyes and Sypro Ruby red post-staining, imaging and image analysis using DeCyder software, robotic gel spot picking, chemical processing and protease digestion, and peptide mass fingerprinting; (3) 1D-SDS-PAGE gel separation of membrane-associated proteins combined with nanoLC-ESI-tandem mass spectrometry; (4) isotope-labeling methods (iTRAQ reagents) to carry out quantitative analysis of the effects of treatments and time on members of the tissue, cell and fluid proteomes; and (5) development of specific multiple reaction ion monitoring (MRM) mass spectrometry methods to quantify peptides that allow quantitative analysis of individual, specified proteins. In addition, the LC-MRM-MS methods can be applied to the analysis of the reagents used in skin disease research, for example, green tea polyphenols and grape seed extract, as well as their and drug metabolites in the blood and other fluids. A special emphasis is made in maintaining quality control of the methods. A Laboratory Information Management System will be used to oversee the operations of the Core. Investigators will be trained individually in the use of the Core techniques as well as at workshops and a Symposium held in conjunction with the other NIH-funded Centers

UAB皮肤病研究中心的蛋白质组学核心D为试点/可行性获奖者提供支持。