"UAB Metabolomics Workshop: from design to decision"

描述(申请人提供)：这是一个申请发展和介绍一个为期4天的代谢组学研讨会。虽然将向与会者介绍代谢组学所有方面的框架，但研讨会的最终目标是使研究人员做好准备，以促进代谢组学在翻译研究中的使用，并指导代谢组学研究中高度跨学科的团队或合作。为此，讲习班将特别注重制定实验设计，以便有意义地开展代谢组学研究。结合这一重点，研讨会将提供代谢组学方面的有组织的实践经验。目标是确保在这一轮组学发展中，与会者将从过去15年的组学研究中学到经验。在第一年，在强有力的机构支持下，讲习班将处于发展阶段，最多只能有16人参加。这是为了仔细测试将占研讨会活动近50%的动手体验，然后将其扩展到将在2-5年内参加完整研讨会的40名与会者。我们将使用第一门课程的演讲者和参与者提供的反馈/建议来改进课程的所有方面-并在每年继续使用反馈意见和对以前的参与者如何使用代谢组学的评估来指导我们确定后续研讨会的内容和形式。研讨会将由五个主要教学部分组成：(1)了解什么是代谢组学/代谢组学以及如何设计代谢组学实验；(2)样本收集和存储中的关键问题；(3)选择代谢组学、质谱学和核磁共振分析工具的理由；(4)收集的代谢组学数据的处理和高维数据的统计分析；以及(5)代谢组学的生物信息学、软件和计算机资源需求。与会者将通过亲身体验了解其中的每一个-他们将处理MS和核磁共振分析的样本，与会者将获得从理想/优化到特别损坏的数据的分析方案。这些协议将被设计为显示糟糕设计的后果。所有样本都将经过处理，以便与会者可以应用他们在教学课程中教授的分析、统计和生物信息学方法。在最后一次会议中，与会者和研讨会教职员工将讨论已获得的数据。将有最多10个学生/博士后奖学金通过竞争性程序在2-5年级的研讨会上颁发。这些费用包括注册费、住宿费/伙食费和去讲习班的旅费。其他学生/博士后、教职员工和非大学参与者将收取分级注册费。 将把重点放在确保两性平等和招聘服务不足的少数群体上。UAB是这个研讨会的绝佳环境，因为它在代谢组学所需的技术上进行了出色的投资，其生物统计学研究人员的实力以及对生物信息学的投资。

DESCRIPTION (provided by applicant): This is an application for the development and presentation of a 4-day Workshop on metabolomics. While the framework of all aspects of metabolomics will be presented to the attendees, the ultimate goal of the Workshop is to prepare investigators to advance the use of metabolomics in translational research and to direct highly interdisciplinary teams or collaborations in metabolomic studies. To that end, the Workshop will have a specific focus on development of experimental designs that will allow for the meaningful execution of metabolomics research. In combination with this emphasis, the Workshop will provide an organized, hands-on experience in metabolomics. The goal is to ensure in this round of -omics development, that attendees will learn from experiences gained from the last 15 years of -omics research. In the first year, with strong institutional support, th Workshop will be in a developmental phase and will be restricted to up to 16 participants. This is to carefully test the hands-on experience that will constitute nearly 50% of the Workshop activities before it is expanded to the 40 attendees who will attend the full Workshop in years 2-5. We will use feedback/advice given by the speakers and attendees of the first course to refine all aspects of the course - and continue throughout each year to use both feedback comments and assessment of how former attendees are using metabolomics to guide us in determining the content and shape of subsequent Workshops. The Workshop will consist of five main didactic sections: (1) understanding what metabolomics/metabonomics is and how to design a metabolomics experiment; (2) critical issues in collection and storage of samples; (3) rationales for choosing the analytical tools of metabolomics, mass spectrometry (MS) and nuclear magnetic resonance (NMR); (4) processing of collected metabolomics data and statistical analysis of high dimensional data; and (5) bioinformatics, software and computer resource needs for metabolomics. Attendees will be introduced to each of these through a hands-on experience - they will process samples for both MS and NMR analysis where attendees will be given analysis protocols that range from ideal/optimized to specifically corrupted data. These protocols will be designed to show the consequence of poor design. All samples will be processed so that the attendees can apply the analytical, statistical and bioinformatic methods they've been taught in the didactic sessions. In the last session, attendees and the Workshop faculty will discuss the data that has been obtained. There will be up to 10 student/postdoc fellowships that will be awarded through a competitive process for Workshops in year 2-5. These will consist of the registration, hotel/food costs and travel to the Workshop. Graded registration fees will apply to the other students/postdocs, faculty and non- university attendees. Emphasis will be placed ensuring gender equality and underserved minority recruitment. UAB is a superb environment for this Workshop because of its excellent investment in the technologies needed for metabolomics, the strength of its biostatistic research faculty and its investment in bioinformatics.