The MEME suite of motif-based sequence analysis tools

项目总结/摘要 DNA和蛋白质序列以复杂的代码数字化地存储有关生物功能的信息， 还没有被完全理解。这个代码的基本单位是序列基序，它被定义为 小的重复的DNA或蛋白质序列模式。例如，可能涉及DNA基序， 在开启或关闭一个基因的转录反应环境线索。A蛋白质基序 可能编码了结合位点的特性，使蛋白质能够发挥其功能。的 基于模体的序列分析软件MEME Suite构建DNA和蛋白质的统计模型 基序，使生物学家能够发现新的基序，寻找已知基序的新实例， to comparise比较motifs图案to each另一个.该提案继续开发和维护MEME套件， 这是世界各地的生物学家经常使用的。这项工作的目标有五个方面：（1） 提高MEME套件的可访问性、可用性和互操作性，（2）扩展MEME 处理组蛋白修饰、甲基化、核小体定位等表观遗传数据的套件 和DNaseI超敏感位点，（3）将各种现有的基于基序的软件工具整合到 MEME套件，（4）通过经验证的增强功能来增强MEME套件所使用的算法，以及 (5)继续改善我们的用户支持服务。

Project Summary/Abstract DNA and protein sequences digitally store information about biological function in a complex code that is not yet fully understood. The fundamental unit of this code is the sequence motif, which is defined as a small, recurring DNA or protein sequence pattern. A DNA motif might be involved, for example, in turning on or off the transcription of a gene in response to environmental cues. A protein motif might encode the properties of the binding site that allows the protein to carry out its function. The MEME Suite of motif-based sequence analysis software builds statistical models of DNA and protein motifs, allowing biologists to discover novel motifs, to search for new instances of known motifs, and to compare motifs to one another. This proposal continues to develop and maintain the MEME Suite, which is in regular use by biologists around the world. The aims of this work are five-fold: (1) to increase the accessiblity, usability and interoperability of the MEME Suite, (2) to expand the MEME Suite to handle epigenetic data regarding histone modifications, methylation, nucleosome positioning and DNaseI hypersensitive sites, (3) to integrate a variety of existing motif-based software tools into the MEME Suite, (4) to augment the algorithms used by the MEME Suite with proven enhancements, and (5) to continue to improve our user support services.