PROTEOMICS

蛋白质组学

• 批准号: 8436447
• 负责人: William L. Carroll
• 金额: $ 6.02万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8436447
• 关键词: Acylation; Biological; Biological Markers; Cancer Center; Cancer Center Support Grant; Cancer Etiology; Cell physiology; Cells; Client; Clinical; Collaborations; Commit; Consultations; Data; Databases; Detection; Development; Disease; Dissociation; Electron Transport; Ensure; Equipment; Fee-for-Service Plans; Feedback; Fees; Funding; Gel; Goals; Individual; Institutes; Instruction; Kaplan Cancer Center; Label; Laboratories; Liquid Chromatography; MALDI-TOF Mass Spectrometry; Malignant Neoplasms; Mass Spectrum Analysis; Medical; Methods; Peptides; Phosphorylation; Phosphorylation Site; Post-Translational Protein Processing; Primary Cell Cultures; Process; Protein Analysis; Proteins; Proteolysis; Proteomics; Proteomics Shared Resource; Recruitment Activity; Relative (related person); Reliance; Research; Research Personnel; Resource Sharing; Resources; Scientist; Screening for cancer; Sequence Determination; Services; Signal Transduction; Site; Structure; Technical Expertise; Techniques; Technology; Technology Assessment; Tissues; Ubiquitination; United States National Institutes of Health; base; cancer therapy; cost effective; design; glycosylation; innovative technologies; instrument; interest; new technology; prevent; programs; protein aminoacid sequence; protein function; protein structure; research study; success; tandem mass spectrometry

PROJECT SUMMARY (See instructions): The Proteomics Shared Resource provides state-of the-art biological mass spectrometry analyses to cancer researchers at NYU to help them identify and characterize proteins of medical importance. In many cases, it is only by studying proteins directly that one can achieve a useful understanding of the processes that underly the functioning of cells and tissues in normal and diseased states such as cancer. The shared resource has the capability to identify single, hundreds or even thousands of proteins in a single analysis, often at subfemtomole levels, by state of the art liquid chromatography-tandem mass spectrometry followed by database searching or de novo sequence determination. In most cases the facility strives to obtain accurate information about the absolute and/or relative quantities of these proteins from cells under various conditions to obtain important information about the functioning of the proteins. The shared resource also has special expertise in characterizing posttranslational modifications of proteins such as phosphorylation, acylation, glycosylation and ubiquitination. In addition to its technical expertise, one of the strengths of the facility is its ability to advise clients in the design and interpretation of experiments so that useful data can be obtained and meaningful information can be had from these data. Reliance on cutting edge technology and its expert and dedicated staff allow these services to be performed in a cost effective manner. Indeed, many experiments by investigators at NYU and the NYU Cancer Institute (NYUCI) could not have been done without the assistance of the Shared Resource. The Resource also has established a Clinical Proteomics Core for the detection of protein and peptide biomarkers for the early detection of cancer.

项目摘要（请参阅说明）：蛋白质组学共享资源为纽约大学的癌症研究人员提供了最新的生物学质谱分析，以帮助他们识别和表征具有医学重要性的蛋白质。在许多情况下，只有直接研究蛋白质才能对正常状态和患病状态（例如癌症）的细胞和组织功能的过程实现有用的理解。共享资源在单个分析中，通常在亚tomole水平下，通过最先进的液相色谱量表质谱法识别单个，数百甚至数千种蛋白质，然后进行数据库搜索或从头序列确定。在大多数情况下，该设施致力于在各种条件下从细胞中获得这些蛋白质的绝对和/或相对量的准确信息，以获取有关蛋白质功能的重要信息。共享资源还具有特殊的专业知识，可以表征蛋白质的翻译后修饰，例如磷酸化，酰化，糖基化和泛素化。除了其技术专长外，该设施的优势之一是它在实验的设计和解释中为客户提供建议，以便可以获得有用的数据，并且可以从这些数据中获得有意义的信息。依靠尖端技术及其专家和敬业的员工允许以成本效益的方式执行这些服务。确实，没有共享资源的帮助，纽约大学和纽约大学癌症研究所（NYUCI）的许多研究者都无法进行。该资源还建立了一种临床蛋白质组学核心，用于检测蛋白质和肽生物标志物以早期检测到癌症。