FLOW CYTOMETRY AND CELL SORTING

项目总结（见说明）： 流式细胞术和细胞分选共享资源是纽约大学癌症研究所（NYUCI）成员的关键技术。流式细胞术可以分析单个细胞的光散射和荧光特性，并对10，000个细胞进行快速、统计学详细分析。保留单个细胞的数据，可以在多个维度上鉴定亚群，显示不同的表型细胞类型。还可以纯化并收集具有特定荧光谱的细胞用于生长或使用细胞分选进行进一步分析。流式细胞仪和细胞分选共享资源为那些无法负担和维护自己的分选仪和分析仪的用户提供了这项技术，以及设计实验和评估实验结果所需的技术专业知识。该机构拥有一台Beckman Coulter MoFlo分选仪（配备4个激光器和多达9种颜色的荧光）、一台Becton Dickinson 5激光14色ARIA II细胞分选仪（位于BSL-2生物安全外壳中，用于原代人类细胞分选）、一台iCyt/SONY Reflection平行细胞分选仪以及一台5激光17色Becton Dickinson LSRII分析仪。共享资源还具有Becton Dickinson FACScalibur和FACScan分析仪。所有细胞分选实验都由训练有素的共享资源工作人员进行，分析仪由经过共享资源工作人员培训的研究人员运行。科学主任还参加培训活动，包括个人咨询、公共研讨会和用户实践培训。该设施在最近12个月期间进行了3，887小时的分析和3，111小时的分类。

PROJECT SUMMARY (See instructions): The Flow Cytometry & Cell Sorting Shared Resource is a critical technology for members of the NYU Cancer Institute (NYUCI). Flow Cytometry allows analysis of the light scattering and fluorescence properties of individual cells and the rapid, statistically detailed analysis of 10,000s of cells. The data on individual cells is retained, and subpopulations can be identified in multiple dimensions, showing distinct phenotypical cell types. Cells with particular fluorescence profiles can also be purified and collected for growth or further analysis using the cell sorting. The Flow Cytometry & Cell Sorting Shared Resource offers users who cannot afford and maintain their own Sorters and Analyzers access to this technology, as well as the technical expertise required to design experiments and evaluate experimental results. The facility has a Beckman Coulter MoFlo sorter with 4 lasers and up to 9 colors of Fluorescence, a Becton Dickinson 5-laser 14-color ARIA II cell sorter in a BSL-2 biosafety enclose for primary human cell sorting, an iCyt/SONY Reflection parallel cell sorter, as well as a 5-laser 17-color Becton Dickinson LSRII analyser. The shared resource also has a Becton Dickinson FACScalibur and FACScan analyzer. All cell sorting experiments are performed by highly-trained shared resource staff, and analyzers are run by investigators who have been trained by the shared resource staff. The scientific director also participate in training activities including individual consultations, public seminars and hands on training of users. The facility performed 3,887 hours of analysis and 3,111 hours of sorting during the most recent 12 month period.