Bioinformatics

生物信息学共享资源（BISR）的使命是提供生物信息学和生物统计学 合作、咨询和定量研究资源，以临床、实验室和预防 从事规划、实施、分析和解释研究的科学家， 分子生物学的高通量技术。此外，BISR的成员还与 肿瘤学研究科学家开发生物信息学和生物统计学方法，以提高效率， 当前和未来的基础科学、治疗、诊断、预防和干预研究。成员 BISR具有使用来自Affyware、Agilent和Illumina mRNA表达微阵列的经验， 蛋白质组学，使用质谱或二维聚丙烯酰胺凝胶电泳;系列分析 基因表达;组织微阵列;阵列比较基因组杂交;单核苷酸 多态性芯片;甲基化芯片; microRNA芯片;外显子拼接芯片;实时聚合酶链 反应;和反相蛋白裂解物阵列。BISR提供选择技术的建议 平台，实验设计，样本量和功效计算，数据预处理，统计 分析和生物信息学解释。资源的服务是针对三个目标：（1） 以确保所有在医学博士的高通量实验安德森的正确分析和解释，（2） 在使用这些技术的实验的规划和设计中发挥直接作用，以及（3）提供 生物信息学方法的教育计划。 在上一个资助期间，BISR协助了来自20个不同项目的244名调查人员。使用 在过去的五年中，BISR翻了一番，BISR用户的出版物数量也在增长 从第一年的33人增加到最近一年的65人。今后，我们将继续致力于 为使用尖端技术的实验设计和分析提供支持， 与医学博士安德森的研究人员了解新技术产生的数据的性质。 在可能的情况下，我们正在通过在 Swave，一种增强统计分析重现性的工具。我们还计划提高我们的能力 提供基于序列的生物信息学服务，如ChIP芯片和基序发现。最后，我们 探索整合数据、算法和报告的创新方法。

The mission of the Bioinformatics Shared Resource (BISR) is to provide bioinformatic and biostatistical collaboration, consultation, and quantitative research resources to clinical, laboratory, and prevention scientists engaged in the planning, conduct, analysis, and interpretation of research studies that use modern high-throughput technologies from molecular biology. Additionally, members of the BISR collaborate with oncology research scientists to develop bioinformatic and biostatistical methods to improve the efficiency of current and future basic science, therapeutic, diagnostic, prevention, and intervention studies. Members of the BISR have experience with mRNA expression microarrays from Affymetrix, Agilent, and Illumina, proteomics using mass spectrometry or two-dimensional polyacrylamide gel electrophoresis; serial analysis of gene expression; tissue microarrays; array comparative genomic hybridization; single nucleotide polymorphism chips; methylation arrays; microRNA arrays; exon tiling arrays; real-time polymerase chain reaction; and reverse-phase protein lysate arrays. The BISR provides advice on choosing technology platforms, experimental design, sample size and power computations, data preprocessing, statistical analysis, and bioinformatic interpretation. The services of the resource are directed toward three goals: (1) to ensure that all high-throughput experiments at M.D. Anderson are properly analyzed and interpreted, (2) to play a direct role in the planning and design of experiments that use these technologies, and (3) to provide educational programs in bioinformatics methods. During the previous funding period, the BISR assisted 244 investigators from 20 different programs. Use of the BISR doubled during the previous five years, and the number of publications by users of the BISR grew from 33 during the first year to 65 during the most recent year. In the future, we remain committed to providing support for the design and analysis of experiments using cutting-edge technologies and will work with M.D. Anderson researchers to understand the nature of the data generated from novel technologies. Where possible, we are standardizing our analyses and report generation by developing templates in Sweave, a tool that enhances the reproducibility of statistical analyses. We also plan to enhance our ability to offer sequence-based bioinformatics services such as ChlP-on-chip and motif finding. Finally, we are exploring innovative ways to integrate data, algorithms, and reports.