Analytical Core

包括脂肪组织在内的内分泌器官产生的激素和 免疫细胞和脂肪细胞在调节能量平衡和葡萄糖代谢中起主要作用。 组织脂质和细胞内脂质衍生代谢物也调节代谢信号通路并影响 胰岛素抵抗了解转基因小鼠代谢表型的基础， 改变代谢，分析核心措施血清和组织因子已知影响能量平衡 和葡萄糖稳态。Analytical Core的目标是提供标准化和高通量的 使用最先进的仪器对从以下组织获得的血清和组织样本进行分析测量： 转基因小鼠核心在大卫哈兰博士的监督下运作， Drs. Randall Friedline、Klaus Pechhold和Scott Shaffer担任联合董事。分析核心提供了 以下服务：1）血清/组织/细胞培养水平的高通量和多重测量 激素、细胞因子和趋化因子，使用Luminex和Meso Scale Discovery多重技术， 2)使用Cobas高通量测量血液/组织/尿液代谢物和电解质水平 临床化学分析仪，3）胰岛素相关细胞内信号通路的分子分析 作用、葡萄糖代谢和炎症，4）测量细胞内脂质水平， 使用三重四极杆质谱法测定脂质衍生的代谢物，和5）胰岛组织学的评估 和胰岛细胞亚群的基因转录分析。分析核心执行质量和 可重复测量，以支持代谢和心血管并发症核心， 转基因小鼠的综合表型。

Hormones produced by endocrine organs including adipose tissue and inflammatory cytokines produced by immune cells and adipocytes play a major role in regulating energy balance and glucose metabolism. Tissue lipid and intracellular lipid-defived metabolites also modulate metabolic signaling pathways and affect insulin resistance. To understand the underlying basis of metabolic phenotypes of transgenic mice with altered metabolism, the Analytical Core measures serum and tissue factors known to affect energy balance and glucose homeostasis. The goal ofthe Analytical Core is to provide standardized and high-throughput analytical measurements using state-of-the-art instruments in serum and tissue samples obtained from transgenic mice. The Core operates under the supen/ision of Dr. David Harlan as the Core Director and Drs. Randall Friedline, Klaus Pechhold, and Scott Shaffer as co-Directors. The Analytical Core provides the following services: 1) High-throughput and multiplexed measurement of serum/tissue/cell culture levels of hormones, cytokines, and chemokines using Luminex and Meso Scale Discovery multiplexing technology, 2) High-throughput measurement of blood/tissue/urine levels of metabolites and electrolytes using a Cobas Clinical Chemistry Analyzer, 3) Molecular analysis of intracellular signaling pathways associated with insulin action, glucose metabolism, and inflammation, 4) Measurement of intracellular levels of lipids and lipid-derived metabolites using triple quadrupole mass spectrometry, and 5) Assessment of islet histology and gene transcriptional analysis of pancreatic islet cell subsets. The Analytical Core performs quality and reproducible measurements to support the Metabolism and Cardiovascular Complications Core for comprehensive phenotyping of transgenic mice.