UMass Mouse Metabolic Phenotyping Center - Metabolism Core

麻省大学小鼠代谢表型中心 - 代谢核心

• 批准号: 9174713
• 负责人: JASON K KIM
• 金额: $ 32.15万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9174713
• 关键词: Acute; Affect; Biochemical; Biological Assay; Biopsy; Blinded; Body Composition; Cardiovascular system; Cell physiology; Chronic; Communities; Conscious; Consumption; Data; Data Analyses; Diabetes Mellitus; Diet; Drug Delivery Systems; Energy Metabolism; Euglycemic Clamping; Exercise; Fatty acid glycerol esters; Food; Genes; Glucose; Glucose Clamp; Glycogen; Glycolysis; Goals; Gold; High Fat Diet; Histology; Hormones; Housing; Human Resources; Hyperglycemia; In Vitro; Individual; Inflammation; Infusion procedures; Injection of therapeutic agent; Insulin; Insulin Resistance; Intravenous; Label; Lipids; Measurement; Measures; Mediating; Metabolic; Metabolism; Methods; Mission; Mus; Obesity; Operative Surgical Procedures; Organ; Pharmaceutical Preparations; Phenotype; Physical activity; Physiological; Plasma; Procedures; Production; Protocols documentation; Pump; Radioactive; Research; Research Personnel; Role; Sampling; Schedule; Serum; Services; Streptozocin; Structure; Structure of beta Cell of islet; Supervision; Techniques; Testing; Tissue Sample; Tissues; Training; Transgenic Mice; Water; Water consumption; abstracting; base; blood glucose regulation; chemokine; cytokine; design; energy balance; feeding; glucose metabolism; glucose tolerance; glucose uptake; human disease; implantation; in vivo; insight; instrument; insulin secretion; insulin tolerance; intraperitoneal; islet; metabolic phenotype; mouse model; operation; programs; research and development; research study; respiratory; treadmill

Project Summary - Abstract The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the purpose of detailed metabolic phenotyping of transgenic mouse models useful in understanding obesity, diabetes and its complications. The Core operates under the supervision of Dr. Jason Kim (Director) and Drs. Roger Davis and Hye-Lim Noh as co-Directors who oversee day-to-day operation of the Core and interact with the users of the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1) hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious mice, 2) hyperglycemic clamp to assess in vivo pancreatic β-cell function in conscious mice, 3) glucose/insulin tolerance tests, 4) survival surgery for in vivo experiments, 5) implementation of osmotic pumps for chronic delivery of drugs or other agents, 6) application of special diet (e.g., high-fat diet) to alter energy balance and induce obesity in mice, 7) acute lipid infusion, acute/chronic delivery of cytokines, and STZ injection to alter glucose homeostasis, 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical activity, and food/water intake in conscious mice using metabolic cages, 9) non-invasive assessment of whole body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1H-MRS, 10) implementation of treadmill and cage wheels for exercise study, 12) biochemical analysis for intracellular metabolic flux measurement (glycolysis, glycogen synthesis), and 13) blinded drug trial studies. The Metabolism Core regularly interacts with the Analytical and Functional Core for complementary, high-throughput analytical assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic experiments. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the role of selected gene on obesity, insulin resistance, and diabetes.

项目摘要-摘要 代谢核心设计用于在清醒小鼠中进行体内和生理实验， 转基因小鼠模型的详细代谢表型分析的目的， 及其并发症。核心在Jason Kim博士（主任）和Roger Davis博士的监督下运作 和Hye-Lim Noh担任共同董事，负责监督Core的日常运营，并与 麻省大学MMPC核心的主要功能是进行以下实验：1） 高胰岛素-正葡萄糖钳夹技术评价清醒大鼠器官特异性胰岛素作用和葡萄糖代谢 小鼠，2）高血糖钳夹以评估清醒小鼠体内胰腺β细胞功能，3）葡萄糖/胰岛素 耐受性测试，4）用于体内实验的存活手术，5）用于慢性炎症的渗透泵的实施， 药物或其它试剂的递送，6）特殊饮食的应用（例如，高脂肪饮食）来改变能量平衡， 诱导小鼠肥胖，7）急性脂质输注，细胞因子急性/慢性递送，和STZ注射以改变 葡萄糖稳态，8）能量消耗的非侵入性测量，呼吸交换率，物理 活动和食物/水摄入，9）非侵入性评估整体 使用1H-MRS的清醒小鼠中脂肪/瘦/水质量的身体、器官和组织活检组成，10） 用于运动研究跑步机和笼轮的实施，12）细胞内的生化分析 代谢通量测量（糖酵解、糖原合成），和13）盲法药物试验研究。代谢 核心定期与分析和功能核心互动，以实现互补的高通量分析 使用代谢试验结束时获得的血清样品测定激素、细胞因子和代谢物的测定法 实验代谢核心的目标是使用一系列优雅，非侵入性和体内实验 为了从个体小鼠获得一组稳健的代谢数据，以便提供对代谢的机制性见解， 选定基因在肥胖、胰岛素抵抗和糖尿病中的作用。