Rhesus Monkey Oocyte Resources

描述(申请人提供)：由于人类和非人类灵长类动物具有许多独特的生殖特征，特别是在卵泡选择和功能、卵母细胞成熟和早期胚胎生长方面，恒河猴模型对于可以直接应用于人类的生殖研究尤其关键，特别是在确定成人疾病的胎儿起源的研究中。这一资源将是卵母细胞和卵泡细胞的来源，以及方案和培训的来源，供目前利用或希望将其研究扩展到灵长类模型的研究人员使用，以促进向人类应用的转换。此外，缺乏可靠、充足的非人类灵长类卵母细胞是阻碍早期胚胎发育、受精、转基因、胚胎干细胞系和产生遗传相同后代的非人类灵长类动物模型的主要问题。改进的非人类灵长类卵母细胞体外成熟(IVM)和冷冻保存技术将有助于缓解这一不足，并提高这一人类健康重要模型的研究效率。开发这一资源的目的是向灵长类中心以外的调查人员提供服务和样本，以促进翻译研究。资源的使用可能包括由NICHD、NCRR、NIEHS、NIA、NIAAA等一系列分类研究所资助的调查人员。对于其他动物模型和人类之间的翻译研究桥梁来说，提供获取方案、培训，并最终建立非人类灵长类卵母细胞、卵泡细胞、组织和裂解物库是必不可少的。因此，该项目的总体目标是为继续开发具有发育能力的体外成熟的非人类灵长类卵母细胞的技术和长期保存非人类灵长类卵母细胞的冷冻保存方法提供方案和培训。长期目标将是提供NIH资助的研究人员可以访问的卵母细胞和辅助细胞库，以及卵巢组织和细胞裂解物。该项目的具体目标将是：1)为恒河猴卵母细胞的体外成熟和冷冻保存提供当前的方案和培训；2)建立卵母细胞和体细胞辅助细胞库(卵丘和颗粒)，作为冷冻材料、固定材料和裂解物分布，用于分子分析。新鲜的卵母细胞和体细胞附属细胞也将可供运输；3)满足人类和非人类生殖生物学方面加强卵母细胞冷冻保存和体外培养方法的迫切需求。 公共卫生相关性(由申请者提供)：恒河猴是与人类健康高度相关的灵长类动物模型。灵长类卵母细胞特有资源的开发将是推动小鼠以外许多领域的进步并应用于人类的关键。开发新的转基因疾病模型以及干细胞治疗或遗传编程的新技术将需要大量的恒河猴卵母细胞，这样翻译研究工作才会成功。

DESCRIPTION (provided by applicant): Because human and nonhuman primates share many unique characteristics of reproduction, especially in regard to follicle selection and function, oocyte maturation and early embryo growth, the rhesus model is especially critical for research in reproduction that can have direct applications to humans, especially in research to determine fetal origins of adult disease. This resource would be a source of oocytes and follicle cells, as well as protocols and training, for investigators that currently utilize or that want to expand their studies into a primate model to facilitate translation to human applications. Furthermore, the lack of a reliable, adequate supply of non-human primate oocytes is a major problem impeding progress in non- human primate models of early embryo development, fertilization, transgenics, embryonic stem cell lines, and production of genetically identical offspring. Improved technologies for in vitro maturation (IVM) and cryopreservation of non-human primate oocytes would help alleviate this shortage and increase the efficiency of research in this important model for human health. This resource will be developed for the purpose of providing services and samples to investigators that are outside of the Primate Centers to facilitate translational research. Use of the resource will likely encompass investigators funded by a range of categorical institutes including NICHD, NCRR, NIEHS, NIA, NIAAA. Providing access to protocols, training and, ultimately, a "bank" of nonhuman primate oocytes, follicles cells, tissues and lysates is essential for the translational research bridge between other animal models and humans. Therefore, the general objectives of this project are to provide protocols and training for continually developing techniques for production of developmentally competent in vitro matured nonhuman primate oocytes and cryopreservation methods for the long-term storage of nonhuman primate oocytes. The long-term goal will be to provide a bank of oocytes and accessory cells as well as ovarian tissue and cell lysates that can be accessed by NIH funded investigators. The specific aims of the project will be 1) Provide current protocols and training for in vitro maturation and cryopreservation of rhesus monkey oocytes; 2) Develop a bank of oocytes and somatic accessory cells (cumulus and granulosa) for distribution as cryopreserved material, fixed material, and lysates for molecular analyses. Fresh oocytes and somatic accessory cells will also be available for shipment; 3) Address the urgent need in both human and nonhuman reproductive biology to enhance oocyte cryopreservation and IVM methods. PUBLIC HEALTH RELEVANCE (provided by applicant): The rhesus macaque is a primate model that is highly relevant to human health. The development of resources specific for primate oocytes will be essential for moving advances in many fields beyond the mouse and toward application in humans. The development of new transgenic models of disease as well as new techniques for stem cell therapy or genetic programming will require large numbers of rhesus oocytes so that translational research efforts will be successful.