RHESUS MONKEY OOCYTE RESOURCES

恒河猴卵母细胞资源

• 批准号: 8357359
• 负责人: CATHERINE A VANDEVOORT
• 金额: $ 7.56万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357359
• 关键词: Address; Antigen-Presenting Cells; Barker Hypothesis; California; Cells; Characteristics; Cryopreservation; Embryo; Funding; Goals; Grant; Growth; Human; In Vitro; Macaca mulatta; Methods; Modeling; Molecular; National Center for Research Resources; Oocytes; Ovarian Tissue; Primates; Principal Investigator; Protocols documentation; Reproduction; Reproductive Biology; Research; Research Infrastructure; Research Personnel; Resources; Source; Tern; Training; Translations; United States National Institutes of Health; cost; direct application; investigator training; nonhuman primate; oocyte maturation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Because human and nonhuman primates share many unique characteristics of reproduction, especially in regard to follicle selection and function, oocyte maturation and early embryo growth, the rhesus model is especially critical for research in reproduction that can have direct applications to humans, especially in research to determine fetal origins of adult disease. This resource would be a source of oocytes and follicle cells, as well as protocols and training, for investigators that currently utilize or that want to expand their studies into a primate model to facilitate translation to human applications. The long-tern goal will be to provide a bank of oocytes and accessory cells as well as ovarian tissue and cell lysates that can be accessed by NIH funded investigators. The specific aims of the project will be 1) Provide current protocols and training for in vitro maturation and cryopreservation of rhesus monkey oocytes; 2) Develop a bank of oocytes and somatic accessory cells (cumulus and granulosa) for distribution as cryopreserved material, fixed material, and lysates for molecular analyses. Fresh oocytes and somatic accessory cells will also be available for shipment; 3) Address the urgent need in both human and nonhuman reproductive biology to enhance oocyte cryopreservation and IVM methods.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 由于人类和非人类灵长类动物具有许多独特的生殖特征，特别是在卵泡选择和功能、卵母细胞成熟和早期胚胎生长方面，恒河猴模型对于可以直接应用于人类的生殖研究尤其关键，特别是在确定成人疾病的胎儿起源的研究中。这一资源将是卵母细胞和卵泡细胞的来源，以及方案和培训的来源，供目前利用或希望将其研究扩展到灵长类模型的研究人员使用，以促进向人类应用的转换。长期目标将是提供NIH资助的研究人员可以访问的卵母细胞和辅助细胞以及卵巢组织和细胞裂解物的库。该项目的具体目标将是：1)为恒河猴卵母细胞的体外成熟和冷冻保存提供当前的方案和培训；2)建立卵母细胞和体细胞辅助细胞库(卵丘和颗粒)，作为冷冻材料、固定材料和裂解物分布，用于分子分析。新鲜的卵母细胞和体细胞附属细胞也将可供运输；3)满足人类和非人类生殖生物学方面加强卵母细胞冷冻保存和体外培养方法的迫切需求。