RHESUS MONKEY OOCYTE RESOURCES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 由于人类和非人类灵长类动物具有许多独特的生殖特征，特别是在卵泡选择和功能，卵母细胞成熟和早期胚胎生长方面，恒河猴模型对于可以直接应用于人类的生殖研究特别重要，特别是在确定成人疾病的胎儿起源的研究中。该资源将成为卵母细胞和卵泡细胞的来源，以及方案和培训，用于目前利用或希望将其研究扩展到灵长类动物模型的研究人员，以促进转化为人类应用。长期目标是提供一个卵母细胞和辅助细胞库，以及卵巢组织和细胞裂解物，供NIH资助的研究人员使用。该项目的具体目标是：1）提供恒河猴卵母细胞体外成熟和冷冻保存的现行方案和培训; 2）开发卵母细胞和体细胞辅助细胞（卵丘和颗粒细胞）库，作为冷冻保存材料、固定材料和裂解物进行分子分析。新鲜的卵母细胞和体细胞辅助细胞也将可用于运输; 3）解决人类和非人类生殖生物学对增强卵母细胞冷冻保存和IVM方法的迫切需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Because human and nonhuman primates share many unique characteristics of reproduction, especially in regard to follicle selection and function, oocyte maturation and early embryo growth, the rhesus model is especially critical for research in reproduction that can have direct applications to humans, especially in research to determine fetal origins of adult disease. This resource would be a source of oocytes and follicle cells, as well as protocols and training, for investigators that currently utilize or that want to expand their studies into a primate model to facilitate translation to human applications. The long-tern goal will be to provide a bank of oocytes and accessory cells as well as ovarian tissue and cell lysates that can be accessed by NIH funded investigators. The specific aims of the project will be 1) Provide current protocols and training for in vitro maturation and cryopreservation of rhesus monkey oocytes; 2) Develop a bank of oocytes and somatic accessory cells (cumulus and granulosa) for distribution as cryopreserved material, fixed material, and lysates for molecular analyses. Fresh oocytes and somatic accessory cells will also be available for shipment; 3) Address the urgent need in both human and nonhuman reproductive biology to enhance oocyte cryopreservation and IVM methods.