Validation of carbamate pesticide screen for VetLIRN network

CAHFS摘要 加州动物健康和食品安全实验室系统的毒理学部分是 为FDA兽医中心的资助机会提交拨款申请 公告编号PA-13-244，标题：“扩大兽医-LIRN合作协议计划 《动物诊断样本中食物污染物的测试方法》（U18）。 CAHFS毒理学是Vet-LIRN网络的成员。我们申请了整整5年的 经费我们建议，在资助的头三年内，我们将验证 饲料、瘤胃（或瘤胃）中氨基甲酸酯农药的定性筛选方法的性能 胃）内容物和肝脏。氨基甲酸酯是剧毒杀虫剂， 注册。它们在农业和家庭环境中都有发现。这些矩阵 我们在诊断实验室中经常遇到的，代表常见的基质 可能是从动物接触环境中收集的。在第四年，我们建议 验证肝脏中蓖麻碱的分析方法。蓖麻碱是一种 生化恐怖分子蓖麻毒素在第五年，我们建议验证一种方法， 胃内容物中的霉菌毒素罗克福汀和青霉烯-A。 对于每种方法，我们建议遵循AOAC指南第三级：多实验室 验证。虽然这些指南要求至少有两个实验室验证一种方法，但我们 建议至少有三个实验室在此验证工作中进行合作。方法都是 定性，并且全部在LC/MS平台上进行。这些的性能特点 由于不同的离子源之间的离子源设计的差异， 厂商因此，在各种品牌的仪器上验证方法 将增强方法的稳健性，并确保它们将广泛适用于 其他Vet-LIRN成员实验室。

CAHFS ABSTRACT The toxicology section of the California Animal Health and Food Safety Laboratory System is submitting a grant proposal for the FDA Center for Veterinary Medicine's Funding Opportunity Announcement number PA-13-244, titled:" Vet-LIRN Cooperative Agreement Program to Expand and Validate Testing Methods for Food Contaminants in Animal Diagnostic Specimens (U18)". CAHFS Toxicology is a member of the Vet-LIRN network. We are applying for a full 5 years of funding. We propose that within the first three years of funding we will validate the performance of a qualitative screening method for carbamate pesticides in feed, rumen (or stomach) content and liver. Carbamates are highly toxic insecticides, with current pesticide use registrations. They are found both in agricultural and home environments. These matrices are ones we commonly encounter in our diagnostic laboratory, and represent the common matrices that may be collected from an animal exposure situation. During the fourth year we propose validating a method for the analysis of Ricinine in liver. Ricinine is a biomarker of the bioterrorism agent Ricin. During the fifth year we propose validating a method for the mycotoxins Roquefortine and Penitrem-A in stomach content. For each method we propose following the AOAC guidelines Level Three: Multi-Laboratory Validation. While these guidelines require that at minimum two labs validate a method, we are proposing at least three labs collaborate in this validation effort. The methods are all qualitative, and are all performed on an LC/MS platform. Performance characteristics of these instruments can vary widely due to differences in ion source design between different manufacturers. Therefore, having the methods validated on a variety of brands of instruments will enhance the robustness of the methods and ensure that they will be widely applicable in other Vet-LIRN member laboratories.