Validating Multiple Stable Isotope Measures of Diet in the NPAAS Feeding Study

在 NPAAS 喂养研究中验证饮食的多种稳定同位素测量

• 批准号: 8623066
• 负责人: Diane Marie O'Brien
• 金额: $ 24.64万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-17 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8623066
• 关键词: Address; Adoption; Age; Alanine; Alaska Native; Amino Acids; Animals; Biological Markers; Canes; Canned Foods; Carbon; Carbon Isotopes; Chronic Disease; Development; Diet; Dietary Assessment; Dietary Intervention; Dietary Practices; Dimensions; Disease Association; Epidemiologic Studies; Fishes; Food; Health; Individual; Intake; Isotopes; Linear Models; Link; Marines; Mass Spectrum Analysis; Measures; Meat; Methodology; Methods; Modeling; Nitrogen; Nitrogen Isotopes; Nutrient; Parents; Participant; Patient Self-Report; Phenylalanine; Physical activity; Plants; Population; Postmenopause; Relative (related person); Reporting; Research; Sampling; Serum; Source; Specificity; Specimen; Sulfur; Sweetening Agents; Testing; Validation; Variant; Woman; Work; Yup' ik; base; candidate validation; cost effective; design; feeding; improved; instrument; interest; metabolic abnormality assessment; minimally invasive; novel strategies; nutrition; nutritional epidemiology; prevent; public health relevance; research study; stable isotope; sugar; tool

Project Summary This proposal addresses a key barrier to research in nutritional epidemiology: the small number of objective biomarkers available for assessing relationships between diet and health. Because diet-disease associations may be subtle and are difficult to establish using self-reported methods, there is a pressing need to develop and validate more objective dietary biomarkers for use in research. The proposed study will evaluate a set of dietary biomarkers for sweetener, meat and fish intake based on the stable isotope ratios of carbon, nitrogen and sulfur. The carbon isotope ratio is a candidate biomarker of sweetener intake because it is high in corn and sugar cane, the major sources of dietary sweeteners in the US. The nitrogen isotope ratio is a candidate biomarker of meat intake, because it is elevated in animals relative to plants. The sulfur isotope ratio is a candidate biomarker of fish intake, because marine foods are elevated relative to terrestrial foods. However, these measures have not yet been validated in an appropriate framework, which has prevented their adoption as tools for nutritional epidemiology. Here we test two approaches to validating isotopic measures for foods of interest: first, by using multi- isotopic models of diet to increase specificity, and second, by measuring the stable isotope ratios of specific amino acids that are closely linked to those foods. We test these approaches using serum specimens from the NPAAS Feeding Study, a 2-week controlled feeding study of 150 women ages 60-80 in Seattle, WA. The NPAAS Feeding Study individually matches each participant's controlled diet to her usual intake, in order to maintain intake distributions that are similar to those of the study population and achieve the level of control needed for biomarker validation. Specific Aim 1 will test whether single or multi-isotopic models based on serum carbon, nitrogen, and sulfur stable isotope ratios can provide valid and specific measures of sweetener, fish and meat intake in the NPAAS Feeding Study. Specific Aim 2 will test whether the carbon isotope ratio of specific serum amino acids can provide alternatives to multi-isotopic models of similar or improved validity and specificity. All stable isotope ratios will be measured using continuous-flow isotope ratio mass spectrometry. This proposal is a first step toward validating multiple stable isotope biomarkers of diet as new tools for objective dietary assessment. Using samples from the NPAAS Feeding Study provides an efficient and cost-effective opportunity to critically assess and refine these markers for more general use in epidemiologic research.

项目摘要 这项建议解决了营养流行病学研究的一个关键障碍： 目的研究评价饮食与健康关系的生物标志物。因为饮食疾病 关联可能是微妙的，很难使用自我报告的方法建立，但有迫切的需求 开发和验证更客观的饮食生物标记物，用于研究。拟议的研究将 根据甜味剂、肉类和鱼类摄入量的稳定同位素比率，评估一组饮食生物标志物 碳、氮和硫。碳同位素比率是甜味剂摄入量的候选生物标志物，因为 它富含玉米和甘蔗，这是美国饮食甜味剂的主要来源。氮同位素 Ratio是肉类摄入量的一个候选生物标志物，因为相对于植物，动物的Ratio更高。硫磺 同位素比率是鱼类摄入量的一个候选生物标志物，因为相对于陆生食物，海洋食品的摄入量更高。 食物。然而，这些措施尚未在适当的框架中得到验证，这已经 防止将其作为营养流行病学的工具。 在这里，我们测试了两种验证感兴趣食品的同位素测量的方法：第一，通过使用多个 增加饮食的同位素模型的特异性，第二，通过测量稳定的同位素比率的特定 与这些食物密切相关的氨基酸。我们使用来自中国的血清样本测试这些方法 NPAAS喂养研究，对华盛顿州西雅图的150名年龄在60-80岁的女性进行了为期两周的受控喂养研究。这个 NPAAS喂养研究分别将每个参与者的受控饮食与她平时的摄入量进行匹配，以便 保持与研究人群相似的摄入量分布，并达到控制水平 生物标志物验证所需的。特定目标1将测试单同位素模型或多同位素模型是否基于 血清碳、氮和硫稳定同位素比率可以提供有效和具体的测量方法。 NPAAS喂养研究中的甜味剂、鱼和肉的摄入量。《特定目标2》将测试碳排放是否 特定血清氨基酸的同位素比率可以替代相似或相似的多同位素模型 提高了有效性和特异性。所有稳定的同位素比率将使用连续流动的同位素进行测量。 比值质谱学。 这项提议是向验证饮食的多个稳定同位素生物标志物作为新工具迈出的第一步 进行客观的饮食评估。使用来自NPAAS饲料研究的样本提供了一个有效和 具有成本效益的机会对这些标志物进行批判性评估和提炼，以便在流行病学中更普遍地使用 研究。