Animal Core

动物核心

• 批准号: 8589550
• 负责人: MICHAEL J. FORSTER
• 金额: $ 24.97万
• 依托单位: UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8589550
• 关键词: Activities of Daily Living; Address; Adult; Affective; Animal Husbandry; Animals; Brain; Calcium Channel; Cognitive; Data; Databases; Diet; Environment; Equilibrium; Equipment and supply inventories; Estrogen Receptors; Estrogens; Experimental Models; Female; Functional disorder; Generations; Hormonal; Hormone Receptor; Hormones; Human; Implant; Individual; Injury; Instruction; Intervention; Laboratories; Learning; Measures; Memory; Middle Cerebral Artery Occlusion; Modeling; Molecular Target; Monitor; Motor Activity; Mus; Neonatal; Neurological status; Neurons; Operative Surgical Procedures; Outcome Assessment; Ovariectomy; Performance; Phase; Preparation; Procedures; Progesterone; Progesterone Receptors; Psychomotor Performance; Rattus; Regimen; Research; Research Personnel; Resources; Rodent; Silastic; Simulate; Sprague-Dawley Rats; Stroke; Testing; Therapeutic; Time; Tissues; Transgenic Mice; Treatment Protocols; base; behavior test; brain tissue; cognitive function; design; diarylpropionitrile; functional outcomes; functional status; hormone therapy; implantation; instrumentation; mimetics; morris water maze; neonate; novel; pregnant; programs; quality assurance; reproductive; research study; senescence; steroid hormone; young adult

PROJECT SUMMARY (See instructions): The over-arching aim of Core B is to provide to each Project, access to tissue and results relevant to the functional status of ovariectomized rats maintained in two experiments conducted in a standardized setting. In phases I and 11, five treatment regimens [vehicle, estrogen, diarylpropionitrile (DPN), progesterone, or estrogen + progesterone], implemented after 3 different post-ovariectomy delays, will be evaluated for their ability to reverse the functional deficits associated with OVX in young adult (4-month old) and after one delay in reproductive senescent (10 month old) rats. In a subsequent experiment (phase 111), the neuroprotective efficacy of the same treatment regimens will be assessed in the transient middle cerebral artery occlusion (tMCAO) model of stroke. The assessment of functional outcomes at the different post-ovariectomy delays will provide the critical data that allows the individual projects to relate changes in the functions of estrogen receptors, progesterone receptors and intracellular calcium channels, to specific periods of sensitivity or refractoriness to hormone receptor-targeted interventions. Core B will provide standardized implementation of the experimental variables; outcome assessments, environment, and animal husbandry needed for the project experiments, and provide for highly efficient leveraging of resources used by all projects, including staff and animals. To this end. Core B will procure, identify and monitor rats used in the experiments, perform all surgical interventions (ovariectomy, implantation of Silastic pellets and tMCAO), and perform comprehensive analyses of the functional status of the rats in the context of the different treatment regimens. Core B will in addition provide non-behaviorally characterized rats that will have been ovariectomized and exposed to the 5 treatment to Project 2 for synaptoneurosomal fraction preparation, and will maintain availability of neonatal brain tissue for the generation of primary neuronal cultures.

项目总结（见说明）：核心B的总体目标是为每个项目提供与在标准化环境中进行的两项实验中保持的卵巢切除大鼠功能状态相关的组织和结果。在I期和11期，将评价在3次不同的卵巢切除术后延迟后实施的5种治疗方案[溶剂、雌激素、二芳基丙腈（DPN）、孕酮或雌激素+孕酮]逆转年轻成年大鼠（4月龄）和生殖衰老大鼠（10月龄）中与OVX相关的功能缺陷的能力。在随后的实验（111期）中，将在短暂性大脑中动脉闭塞（tMCAO）中风模型中评估相同治疗方案的神经保护功效。在不同的卵巢切除术后延迟的功能结果的评估将提供关键的数据，使个别项目的雌激素受体，孕激素受体和细胞内钙通道的功能变化，以特定时期的敏感性或难治性激素受体靶向干预。核心B将提供实验变量的标准化实施;项目实验所需的结果评估、环境和畜牧业，并提供对所有项目所用资源（包括工作人员和动物）的高效利用。为此目的。核心B将获取、识别和监测实验中使用的大鼠，进行所有手术干预（卵巢切除术、硅橡胶颗粒植入和tMCAO），并在不同治疗方案的背景下对大鼠的功能状态进行综合分析。此外，核心B还将向项目2提供已切除卵巢并暴露于5处理的非行为学表征大鼠，用于制备突触神经体组分，并将维持新生脑组织的可用性，用于生成原代神经元培养物。