Animal Core

动物核心

基本信息

批准号：
8974804
负责人：
MICHAEL J. FORSTER
金额：
$ 30.95万
依托单位：
UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8974804
关键词：
Activities of Daily Living Address Adult Affective Animal Husbandry Animals Brain Calcium Channel Cognitive Data Databases Diet Environment Equilibrium Equipment and supply inventories Estrogen Receptors Estrogens Experimental Models Female Generations Hormonal Hormone Receptor Hormones Human Implant Individual Injury Instruction Intervention Laboratories Learning Measures Memory Middle Cerebral Artery Occlusion Modeling Molecular Target Monitor Motor Activity Mus Neurological status Neurons Operative Surgical Procedures Outcome Assessment Ovariectomy Performance Phase Preparation Procedures Progesterone Progesterone Receptors Psychomotor Performance Rattus Regimen Research Research Personnel Resources Rodent Silastic Sprague-Dawley Rats Stroke Testing Therapeutic Time Tissues Transgenic Mice Treatment Protocols base behavior test brain dysfunction brain tissue cognitive function design diarylpropionitrile functional outcomes functional status hormone therapy implantation instrumentation mimetics morris water maze neonatal brain neonate novel pregnant programs quality assurance reproductive research study senescence steroid hormone young adult

项目摘要

PROJECT SUMMARY (See instructions): The over-arching aim of Core B is to provide to each Project, access to tissue and results relevant to the functional status of ovariectomized rats maintained in two experiments conducted in a standardized setting. In phases I and 11, five treatment regimens [vehicle, estrogen, diarylpropionitrile (DPN), progesterone, or estrogen + progesterone], implemented after 3 different post-ovariectomy delays, will be evaluated for their ability to reverse the functional deficits associated with OVX in young adult (4-month old) and after one delay in reproductive senescent (10 month old) rats. In a subsequent experiment (phase 111), the neuroprotective efficacy of the same treatment regimens will be assessed in the transient middle cerebral artery occlusion (tMCAO) model of stroke. The assessment of functional outcomes at the different post-ovariectomy delays will provide the critical data that allows the individual projects to relate changes in the functions of estrogen receptors, progesterone receptors and intracellular calcium channels, to specific periods of sensitivity or refractoriness to hormone receptor-targeted interventions. Core B will provide standardized implementation of the experimental variables; outcome assessments, environment, and animal husbandry needed for the project experiments, and provide for highly efficient leveraging of resources used by all projects, including staff and animals. To this end. Core B will procure, identify and monitor rats used in the experiments, perform all surgical interventions (ovariectomy, implantation of Silastic pellets and tMCAO), and perform comprehensive analyses of the functional status of the rats in the context of the different treatment regimens. Core B will in addition provide non-behaviorally characterized rats that will have been ovariectomized and exposed to the 5 treatment to Project 2 for synaptoneurosomal fraction preparation, and will maintain availability of neonatal brain tissue for the generation of primary neuronal cultures.

项目摘要（请参阅说明）：核心B的架构目标是为每个项目提供，访问组织以及与在标准化环境中进行的两个实验中维持的卵巢切除大鼠功能状态相关的结果。在第I和第11阶段，将评估3种不同的卵巢切除术后延迟后实施的五种治疗方案（雌激素，雌激素，DPN），孕激素或雌激素 +孕酮]，以逆转年轻人（4个月的年轻人（4个月）延迟（10个月后）的功能赤字，将评估其能力扭转与OVX相关的功能赤字。在随后的实验（第111阶段）中，将在中风的瞬时脑动脉闭塞（TMCAO）模型中评估相同治疗方案的神经保护功效。评估不同卵巢后切除术延迟的功能结果将提供关键数据，使各个项目可以将雌激素受体，孕激素受体和细胞内钙通道功能的变化与特定的敏感性或折磨度与激素受体受体启发性的干预措施相关联。核心B将提供实验变量的标准化实施；项目实验所需的结果评估，环境和畜牧业，并提供了包括员工和动物在内的所有项目所使用的资源的高效利用。为此。 Core B将采购，识别和监测实验中使用的大鼠，执行所有手术干预措施（卵巢切除术，植入silastic Pellets和TMCAO），并在不同治疗方案的背景下对大鼠的功能状态进行全面分析。核心B还将提供非行为表征的大鼠，这些大鼠将被卵巢切除并暴露于5个治疗项目2的治疗方法，以进行突触剂分数的准备，并将维持新生儿脑组织的可用性，以生成原发性神经元培养物。