Animal Core

项目总结（见说明）：核心B的首要目标是为每个项目提供在标准化环境中进行的两个实验中与去卵巢大鼠功能状态相关的组织和结果。在I期和11期，在3个不同的卵巢切除术后延迟后实施的5种治疗方案（对照物、雌激素、二芳基丙腈（DPN）、孕酮或雌激素+孕酮），将评估其在年轻成年大鼠（4个月大）和生殖衰老大鼠（10个月大）中逆转与OVX相关的功能缺陷的能力。在随后的实验（111期）中，将在卒中的短暂性大脑中动脉闭塞（tMCAO）模型中评估相同治疗方案的神经保护效果。对卵巢切除术后不同延迟期的功能结果的评估将提供关键数据，使个体项目能够将雌激素受体、孕激素受体和细胞内钙通道的功能变化与激素受体靶向干预的特定敏感性或难治性联系起来。核心B将提供实验变量的标准化实施；项目实验所需的结果评估、环境和畜牧业，并为所有项目（包括人员和动物）使用的资源提供高效的杠杆作用。为此目的。核心B将获取、识别和监测实验用大鼠，执行所有手术干预（卵巢切除术、硅橡胶颗粒植入和tMCAO），并对不同治疗方案下大鼠的功能状态进行综合分析。Core B还将提供非行为特征的大鼠，这些大鼠将被切除卵巢并暴露于项目2的5处理中，用于突触eurosomal fraction制备，并将保持新生儿脑组织的可用性，以产生初级神经元培养物。

PROJECT SUMMARY (See instructions): The over-arching aim of Core B is to provide to each Project, access to tissue and results relevant to the functional status of ovariectomized rats maintained in two experiments conducted in a standardized setting. In phases I and 11, five treatment regimens [vehicle, estrogen, diarylpropionitrile (DPN), progesterone, or estrogen + progesterone], implemented after 3 different post-ovariectomy delays, will be evaluated for their ability to reverse the functional deficits associated with OVX in young adult (4-month old) and after one delay in reproductive senescent (10 month old) rats. In a subsequent experiment (phase 111), the neuroprotective efficacy of the same treatment regimens will be assessed in the transient middle cerebral artery occlusion (tMCAO) model of stroke. The assessment of functional outcomes at the different post-ovariectomy delays will provide the critical data that allows the individual projects to relate changes in the functions of estrogen receptors, progesterone receptors and intracellular calcium channels, to specific periods of sensitivity or refractoriness to hormone receptor-targeted interventions. Core B will provide standardized implementation of the experimental variables; outcome assessments, environment, and animal husbandry needed for the project experiments, and provide for highly efficient leveraging of resources used by all projects, including staff and animals. To this end. Core B will procure, identify and monitor rats used in the experiments, perform all surgical interventions (ovariectomy, implantation of Silastic pellets and tMCAO), and perform comprehensive analyses of the functional status of the rats in the context of the different treatment regimens. Core B will in addition provide non-behaviorally characterized rats that will have been ovariectomized and exposed to the 5 treatment to Project 2 for synaptoneurosomal fraction preparation, and will maintain availability of neonatal brain tissue for the generation of primary neuronal cultures.