Animal Core

项目摘要(见说明)：核心B的总体目标是为每个项目提供与在标准化环境下进行的两个实验中保持的去卵巢大鼠的功能状态相关的组织和结果。在第一阶段和第十一阶段，将评估五种治疗方案[赋形剂、雌激素、二芳基丙腈(DPN)、孕酮或雌激素孕酮]在卵巢切除后3次不同延迟后实施的能力，以评估它们逆转年轻成年(4个月大)和生殖衰老(10个月大)大鼠与OVX相关的功能缺陷的能力。在随后的实验(111期)中，将在中风的短暂性大脑中动脉闭塞(TMCAO)模型中评估相同治疗方案的神经保护效果。对卵巢切除后不同延迟的功能结果的评估将提供关键数据，使各个项目能够将雌激素受体、孕激素受体和细胞内钙通道功能的变化与激素受体靶向干预的特定敏感期或不敏感期联系起来。核心B将提供项目实验所需的实验变量、结果评估、环境和畜牧业的标准化实施，并高效地利用所有项目使用的资源，包括工作人员和动物。为了这个目的。核心B将获取、识别和监测实验中使用的大鼠，执行所有手术干预(卵巢摘除、硅胶颗粒植入和tMCAO)，并在不同治疗方案的背景下对大鼠的功能状态进行全面分析。此外，CORE B还将提供没有行为特征的大鼠，这些大鼠将被摘除卵巢并暴露在项目2的突触神经体部分准备的5治疗中，并将保持新生脑组织的可用性，以产生原代神经元培养。

PROJECT SUMMARY (See instructions): The over-arching aim of Core B is to provide to each Project, access to tissue and results relevant to the functional status of ovariectomized rats maintained in two experiments conducted in a standardized setting. In phases I and 11, five treatment regimens [vehicle, estrogen, diarylpropionitrile (DPN), progesterone, or estrogen + progesterone], implemented after 3 different post-ovariectomy delays, will be evaluated for their ability to reverse the functional deficits associated with OVX in young adult (4-month old) and after one delay in reproductive senescent (10 month old) rats. In a subsequent experiment (phase 111), the neuroprotective efficacy of the same treatment regimens will be assessed in the transient middle cerebral artery occlusion (tMCAO) model of stroke. The assessment of functional outcomes at the different post-ovariectomy delays will provide the critical data that allows the individual projects to relate changes in the functions of estrogen receptors, progesterone receptors and intracellular calcium channels, to specific periods of sensitivity or refractoriness to hormone receptor-targeted interventions. Core B will provide standardized implementation of the experimental variables; outcome assessments, environment, and animal husbandry needed for the project experiments, and provide for highly efficient leveraging of resources used by all projects, including staff and animals. To this end. Core B will procure, identify and monitor rats used in the experiments, perform all surgical interventions (ovariectomy, implantation of Silastic pellets and tMCAO), and perform comprehensive analyses of the functional status of the rats in the context of the different treatment regimens. Core B will in addition provide non-behaviorally characterized rats that will have been ovariectomized and exposed to the 5 treatment to Project 2 for synaptoneurosomal fraction preparation, and will maintain availability of neonatal brain tissue for the generation of primary neuronal cultures.