Epidemiology and Ecology of Vibrio cholerae in Bangladesh

孟加拉国霍乱弧菌的流行病学和生态学

• 批准号: 8662678
• 负责人: DAVID A SACK
• 金额: $ 182.47万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8662678
• 关键词: Area; Bacteria; Bacteriophages; Bangladesh; Bayesian Analysis; Biological; Biological Assay; Biomass; Biometry; Cells; Characteristics; Chemicals; Chitin; Cholera; Cholera Toxin; Cholera Vaccine; Clinical; Clinical Microbiology; Communicable Diseases; Communities; Competence; Complex; Copepoda; Country; Data; Development; Disease; Disease Outbreaks; Early Mobilizations; Ecology; Elements; Environment; Environmental Risk Factor; Epidemic; Epidemiology; Evolution; Feces; Food; Funding; Genetic; Genome; Genomics; Genotype; Habitats; Horizontal Gene Transfer; Human; India; Individual; Infection; Intervention; Investigation; Laboratories; Licensing; Life; Lytic; Marines; Measurable; Measurement; Measures; Metagenomics; Methods; Microbial Biofilms; Modeling; Molecular; Monitor; Morbidity - disease rate; Morphology; Oral; Patients; Pattern; Personal Satisfaction; Phytoplankton; Plankton; Play; Population; Population Dynamics; Protocols documentation; Public Health; Publishing; Reporting; Research; Research Personnel; Risk; Role; Rural; Salvelinus; Sampling; Sea; Seasons; Serological; Serotyping; Severities; Site; Source; Staging; Surface; Surveys; Technology; Temperature; Time; United States National Institutes of Health; Vaccination; Vibrio cholerae; Vibrio cholerae O1; Water; Water Pollution; Work; Zooplankton; base; chlorophyll a; climate change; cohort; comparative genomics; falls; genetic association; geographically distant; improved; indexing; microbial; microbial community; mortality; pandemic disease; pathogen; predictive modeling; public health emergency; rural area; success; transmission process; trend

DESCRIPTION (provided by investigator): Cholera, caused by Vibrio cholerae serogroups O1 and O139, is the most severe of diarrheal diseases and continues to cause significant morbidity and mortality throughout the developing world. Presently, it is reported in approximately 52 countries worldwide. During our first study, we carried out biweekly clinical and environmental surveillance in four rural areas of Bangladesh. Analysis of our accumulated clinical and environmental data identified factors that are significantly predictive for occurrence of cholera outbreaks, including water temperature, pH, and conductivity of surface water, along with concentrations of plankton and cholera toxin- producing bacteria in surface waters. A significant finding was that zooplankton, namely copepods, serve as an effective host for V. cholerae. By tracking their phytoplankton food source, using satellite monitoring of chlorophyll a concentration, it is possible to estimate zooplankton populations. With our successes to date, we will conduct epidemiological and ecological surveillance in Chattak and Mathbaria, two widely separated geographical sites, where we have documented predictable seasonal cholera outbreaks: one in the spring and the other in the fall. Using improved assays, we will enumerate multiple morphologies and life stages of V. cholerae in environmental samples, including the viable but nonculturable form (VBNC). Characterization of genetic associations between clinical and environmental isolates and GIS surveillance will be conducted to establish key epidemiological relationships that have been difficult to document at the genomic level. To elucidate local variability in cholera outbreaks, we will investigate factors recognized from our previous study, as well as those identified by other investigators, but not yet included in our predictive model, namely microbial community dynamics, increased competence of V. cholerae attached to chitinous surfaces, and plankton community dynamics. Using environmental variables identified from our previous studies and incorporating the new variables that prove to be correlated with cholera cases, we will refine our model of cholera transmission with the intent to render it useful for predicting cholera outbreaks, thereby allowing early mobilization of preventative and treatment measures. The very recent availability of inexpensive oral cholera vaccines makes this objective even more relevant.

描述（由研究人员提供）：霍乱是由弧菌霍乱血清群O1和O139引起的，是最严重的腹泻疾病，并继续在整个发展中国家引起明显的发病率和死亡率。目前，在全球大约52个国家 /地区据报道。在我们的第一个研究中，我们在孟加拉国四个农村地区进行了两周的临床和环境监测。对我们累积的临床和环境数据的分析确定了对霍乱暴发的发生可显着预测的因素，包括水温，pH和地表水的电导率，以及浮游生物和霍乱毒素在地表水中产生细菌的浓度。一个重要的发现是，浮游动物（即copepods）是V.霍乱的有效宿主。通过使用叶绿素浓度的卫星监测来跟踪其浮游植物食品来源，可以估计浮游动物种群。 迄今为止，我们的成功将在Chattak和Mathbaria中进行流行病学和生态监测，这是两个广泛分开的地理地点，我们记录了可预测的季节性霍乱疫情：一个在春季，另一个在秋天。使用改进的测定法，我们将列举在环境样本中的多种形态和霍乱弧菌的生命阶段，包括可行但不可培养的形式（VBNC）。将进行临床和环境分离株和GIS监测之间的遗传关联的表征，以建立在基因组水平上很难记录的关键流行病学关系。 为了阐明霍乱暴发的局部变异性，我们将研究从我们先前的研究中认可的因素，以及其他研究人员确定的因素，但尚未包括在我们的预测模型中，即微生物群落动态，增强了霍乱的霍乱能力，与Chitounity Surfaces相关的V. cholerae的能力，以及Plankton社区动力学。使用从我们以前的研究中鉴定出的环境变量，并结合了被证明与霍乱病例相关的新变量，我们将完善我们的霍乱传播模型，目的是使其对预测霍乱疫情有用，从而允许早期动员预防和治疗措施。廉价的口服霍乱疫苗的最新可用性使该目标更加相关。

项目成果

Genomic diversity among Vibrio cholerae O139 strains isolated in Bangladesh and India between 1992 and 1998.

1992 年至 1998 年间在孟加拉国和印度分离的霍乱弧菌 O139 菌株的基因组多样性。

• DOI: 10.1111/j.1574-6968.2000.tb09027.x
• 发表时间: 2000
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Faruque,SM;Saha,MN;Asadulghani;Bag,PK;Bhadra,RK;Bhattacharya,SK;Sack,RB;Takeda,Y;Nair,GB
• 通讯作者: Nair,GB

Genetic characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes.

霍乱弧菌 O1 的 Matlab 变种的遗传特征，它是经典生物型和埃尔托生物型之间的杂交种。

• 发表时间: 2006
• 期刊: J Med Microbiol 55
• 作者: Liu;Y.;Nakahara;T.;Miyakoshi;J.;Hu;D.-L.;Nakane;A.;Abe;Y.;Zhao et al.;Safa A
• 通讯作者: Safa A

Adaptation of a simple dipstick test for detection of Vibrio cholerae O1 and O139 in environmental water.

• DOI: 10.3389/fmicb.2013.00320
• 发表时间: 2013
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Chakraborty S;Alam M;Scobie HM;Sack DA
• 通讯作者: Sack DA

Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons.

• DOI: 10.1016/j.resmic.2013.06.005
• 发表时间: 2013-10
• 期刊: RESEARCH IN MICROBIOLOGY
• 影响因子: 2.6
• 作者: Cantet, Franck;Hervio-Heath, Dominique;Caro, Audrey;Le Mennec, Cecile;Monteil, Caroline;Quemere, Catherine;Jolivet-Gougeon, Anne;Colwell, Rita R.;Monfort, Patrick
• 通讯作者: Monfort, Patrick

Emergence of multidrug-resistant strain of Vibrio cholerae O1 in Bangladesh and reversal of their susceptibility to tetracycline after two years.

孟加拉国出现多重耐药霍乱弧菌 O1 菌株，两年后其对四环素的敏感性逆转。

• 发表时间: 2007
• 期刊: Journal of health, population, and nutrition
• 作者: Faruque,AbuSG;Alam,Khorshed;Malek,MohammadA;Khan,MohammedGY;Ahmed,Sabeena;Saha,Debasish;Khan,WasifA;Nair,GopinathB;Salam,MohammedA;Luby,StephenP;Sack,DavidA
• 通讯作者: Sack,DavidA