VALIDATION OF CHOLERA IN VOLUNTEERS USING FROZEN BACTERIA FOR CHALLENGE

五名健康的成年社区志愿者参加了一次致命的霍乱 约翰·霍普金斯大学疫苗测试中心的挑战性研究 挑战发生在9月10日这是第四组测试， 在VTU的这一挑战和第三次接受5个log的剂量。 的 制备并施用9.9 × 10 - 4的接种物。 五分之四 志愿者在研究期间出现腹泻，所有人都用 补液其中三名志愿者发烧了。 五个中的四个 志愿者在第1天至第2天期间粪便中反复呈V型胆固醇阳性， 4，但粪便培养在1 - 2分钟内变为霍乱弧菌阴性， 开始四环素治疗的天数 一个在研究期间没有大便 所以只对直肠拭子进行了培养，结果是阴性的。 这 一名志愿者在开始使用四环素后不久也发生了尿道炎， 换成了环丙沙星

Five healthy adult community volunteers participated in a virulent cholera challenge study at the Johns Hopkins University Vaccine Testing Unit with challenge occurring on September 10. This is the fourth group tested with this challenge at the VTU and the third receiving a dose of 5 logs. The inoculum of 9.9 X 10 4 was prepared and administered. Four of five volunteers developed diarrhea during the study, and all were managed with rehydration fluids. Three of the volunteers developed a fever. Four of 5 volunteers had stools repeatedly positive for V cholerae during days 1 to 4, but the stool cultures became negative for v. cholerae within one - two days of starting tetracycline. One did not have a stool during the study so only rectal swabs were cultured and these were negative. This volunteer also developed pruritis shortly after starting tetracycline and was switched to ciprofloxacin.