VALIDATION OF CHOLERA IN VOLUNTEERS USING FROZEN BACTERIA FOR CHALLENGE

使用冷冻细菌进行挑战的志愿者中霍乱的验证

• 批准号: 6245486
• 负责人: DAVID A SACK
• 金额: $ 2.23万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-05 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6245486
• 关键词: Vibrio cholerae; antibacterial agents; body fluid balance therapy; cholera; cholera vaccine; clinical research; communicable disease transmission; cryopreservation; human subject; microorganism disease chemotherapy; tetracyclines; virulence; volunteer

Five healthy adult community volunteers participated in a virulent cholera challenge study at the Johns Hopkins University Vaccine Testing Unit with challenge occurring on September 10. This is the fourth group tested with this challenge at the VTU and the third receiving a dose of 5 logs. The inoculum of 9.9 X 10 4 was prepared and administered. Four of five volunteers developed diarrhea during the study, and all were managed with rehydration fluids. Three of the volunteers developed a fever. Four of 5 volunteers had stools repeatedly positive for V cholerae during days 1 to 4, but the stool cultures became negative for v. cholerae within one - two days of starting tetracycline. One did not have a stool during the study so only rectal swabs were cultured and these were negative. This volunteer also developed pruritis shortly after starting tetracycline and was switched to ciprofloxacin.

五名健康​​成人社区志愿者参与了一场致命的霍乱 约翰·霍普金斯大学疫苗测试中心的挑战研究 挑战发生在 9 月 10 日。这是第四组接受测试的 这次挑战是在 VTU 进行的，第三次挑战接受了 5 log 的剂量。 这 制备并施用9.9×10 4 的接种物。 五之四 志愿者在研究期间出现腹泻，所有志愿者都接受了治疗 补液。三名志愿者出现发烧症状。 5 个中的 4 个 志愿者在第 1 天至第 1 天期间粪便中反复出现霍乱弧菌阳性 4，但一到两秒内粪便培养物霍乱弧菌呈阴性 开始使用四环素的天数。 研究期间没有大便 因此只进行了直肠拭子培养，结果呈阴性。 这 志愿者在开始四环素治疗后不久也出现瘙痒症 改用环丙沙星。