Novel Spectinamide Antibiotics for the Treatment of MDR/XDR Tuberculosis

用于治疗 MDR/XDR 结核病的新型 Spectinamide 抗生素

• 批准号: 8436177
• 负责人: Michelle M. Butler
• 金额: $ 29.11万
• 依托单位: MICROBIOTIX, INC
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2014-05-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8436177
• 关键词: AIDS/HIV problem; Adverse effects; Advocate; Aerosols; Amikacin; Aminoglycosides; Amoxicillin; Anti-Bacterial Agents; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimycobacterial Agents; Antitubercular Agents; Back; Bacteria; Bacterial Proteins; Binding; Biological Assay; Biological Factors; Capromycin; Cause of Death; Cell Line; Cells; Cessation of life; Characteristics; Chemicals; Chemistry; Clarithromycin; Clavulanate; Clinical; Collaborations; Communicable Diseases; Death Rate; Developing Countries; Development; Disease; Dose; Drug Kinetics; Drug Resistant Tuberculosis; Ethambutol; Extreme drug resistant tuberculosis; FDA approved; Fluoroquinolones; Goals; HIV; Human; Imipenem; Immunocompromised Host; In Vitro; Incidence; Individual; Infection; Injectable; Kanamycin; Laboratories; Lead; Linezolid; Location; Mammalian Cell; Mammals; Medical; Metabolic; Modification; Molecular Weight; Moxifloxacin; Multi-Drug Resistance; Multidrug-Resistant Tuberculosis; Mus; Mycobacterium tuberculosis; Neisseria gonorrhoeae; Patients; Pharmaceutical Preparations; Pharmacology; Phase; Population; Process; Production; Property; Protein Biosynthesis; Protein Synthesis Inhibition; Pyrazinamide; Research; Research Project Grants; Resistance; Ribosomes; Rifampin; Route; Safety; Saint Jude Children' s Research Hospital; Series; Site; Solubility; Spectinomycin; Streptomycin; Synthesis Chemistry; Testing; Therapeutic; Therapeutic Agents; Therapeutic Index; Time; Toxic effect; Toxicology; Treatment Protocols; Tuberculosis; Virulent; World Health Organization; alternative treatment; analog; bactericide; clinical material; compliance behavior; cytotoxicity; drug discovery; improved; in vitro activity; in vivo; isoniazid; macrophage; novel; pathogen; pre-clinical; preclinical study; research study; resistant strain; scaffold; scale up; treatment duration; tuberculosis treatment

DESCRIPTION (provided by applicant): The overall objective of this project is to further develop a novel chemical class of tuberculosis (TB) therapeutic agents, the spectinamide series, for use against MDR and XDR strains of Mycobacterium tuberculosis (Mtb) and identify, through a series of in vitro and in vivo assays, a preclinical candidate. TB is a lethal infectious disease, second only to HIV/AIDS as a cause of death. Estimates by the World Health Organization show one-third of the world population is infected with Mtb, the bacterium that causes TB; approximately 10% of infected individuals will develop active TB at some time in their lives. Of recent concern is the rising number of TB cases involving strains that are multidrug-resistant (MDR), which is defined as being resistant to treatment with isoniazid and rifampicin, the two first-line antibiotics for TB therapy and those that are extensively drug-resistant tuberculosis [XDR TB; defined as being resistant to isoniazid and rifampicin (as for MDR TB) and also to any fluoroquinolone and at least one of the three injectable second-line drugs (i.e., amikacin, kanamycin, or capreomycin)]. Treatment options for MDR/XDR TB include the two remaining first- line agents, ethambutol and pyrazinamide, second-line agents to which the strain is not resistant, such as streptomycin, as well as unproven agents such as linezolid, amoxicillin/clavulanate, clarithromycin, clofazimine and imipenem. Research suggests that at least four drugs and 18-24 month duration of treatment may be required for successful therapy of XDR TB; however, toxicity of the agents may preclude patients from being effectively treated. There is a clear unmet medical need for efficacious and safe drugs to be used as treatment for MDR/XDR TB. The novel spectinomycin analogs outlined in this proposal possess potent in vitro activity against MDR/XDR TB (MIC 0.4-1.6 5g/mL), act via a bactericidal mechanism of action, have demonstrated ability to enter macrophages, the host location of TB infections, and are safe compounds, displaying low in vitro cytotoxicity and no observed in vivo toxicity. These overall characteristics advocate for the rapid development of these compounds as a safe alternative treatment for drug resistant TB. We will, in conjunction with our collaborators, Dr. Richard Lee and Helena Boshoff, characterize the in vitro properties of the spectinamide class, including potency against MDR/XDR TB isolates, minimum toxicity against mammalian cell lines and metabolic stability. We will, then scale-up the synthesis of the most favorable analogs for in vivo studies and optimize the synthetic chemistry route in the process. Finally, in collaboration with Dr. Anne Lenaerts, we will evaluate in vivo toxicology, pharmacokinetics and efficacy in mice. These experiments will allow us to identify a final lead and backup compound suitable for advancement to Phase II. In Phase II, we will conduct long term in vivo efficacy studies of the preclinical candidate, both as monotherapy and in combination with anti-TB agents for MDR-TB. We will also conduct pharmacokinetic studies, GLP toxicology and safety pharmacology studies to select a final anti-MDR/XDR clinical candidate suitable for IND submission.

描述（由申请人提供）：本项目的总体目标是进一步开发一种新型化学类别的结核病（TB）治疗药物，壮观酰胺系列，用于抗结核分枝杆菌（Mtb）的MDR和XDR菌株，并通过一系列体外和体内试验鉴定临床前候选药物。结核病是一种致命的传染病，是仅次于艾滋病毒/艾滋病的第二大死因。世界卫生组织的估计显示，世界人口的三分之一感染了结核杆菌，这是一种导致结核病的细菌;大约10%的感染者会在一生中的某个时候患上活动性结核病。最近令人担忧的是，涉及多药耐药（MDR）菌株的结核病例数量不断增加，MDR菌株被定义为对异烟肼和利福平（结核治疗的两种一线抗生素）治疗具有耐药性，以及广泛耐药结核[XDR TB;定义为对异烟肼和利福平（如MDR TB）以及对任何氟喹诺酮和三种可注射二线药物中的至少一种（即，阿米卡星、卡那霉素或卷曲霉素）]。MDR/XDR TB的治疗选择包括剩余的两种一线药物乙胺丁醇和吡嗪酰胺，菌株不耐的二线药物如链霉素，以及未经证实的药物如利奈唑胺、阿莫西林/克拉维酸、克拉霉素、氯法齐明和亚胺培南。研究表明，成功治疗XDR TB可能需要至少4种药物和18-24个月的治疗时间;然而，药物的毒性可能会使患者无法得到有效治疗。对于用于治疗MDR/XDR TB的有效且安全的药物，存在明显未满足的医疗需求。本提案中概述的新型大观霉素类似物具有针对MDR/XDR TB的有效体外活性（MIC 0.4-1.6 5g/mL），通过杀菌作用机制起作用，已经证明能够进入巨噬细胞（TB感染的宿主位置），并且是安全的化合物，显示出低体外细胞毒性并且没有观察到体内毒性。这些总体特征支持快速开发这些化合物作为耐药结核病的安全替代治疗。我们将与我们的合作者Richard Lee博士和Helena Boshoff一起，表征壮观酰胺类的体外特性，包括对MDR/XDR TB分离株的效力，对哺乳动物细胞系的最小毒性和代谢稳定性。然后，我们将扩大用于体内研究的最有利类似物的合成，并优化该过程中的合成化学路线。最后，与Anne Lenaerts博士合作，我们将评估小鼠体内毒理学、药代动力学和疗效。这些实验将使我们能够确定最终的铅和备份化合物适合推进到第二阶段。在第二阶段，我们将对临床前候选药物进行长期体内疗效研究，包括单药治疗和与抗结核药物联合治疗耐多药结核病。我们还将进行药代动力学研究、GLP毒理学和安全药理学研究，以选择适合IND提交的最终抗MDR/XDR临床候选药物。