Non-invasive Imaging of the In Situ Restoration of Brain Tissue

脑组织原位修复的无创成像

基本信息

批准号：
8756142
负责人：
Michel M. Modo
金额：
$ 29.89万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8756142
关键词：
Address Affect American Biocompatible Materials Biological Blood flow Brain Brain Injuries Cell Fraction Cell Therapy Cell Transplantation Cells Characteristics Chemicals Clinical Trials Contrast Media Detection Development Diffusion Diffusion Magnetic Resonance Imaging Disease Endothelial Cells Ensure Environment Evolution Extracellular Matrix Goals Head Human Image Imagery Imaging Techniques Implant In Situ Injection of therapeutic agent Investigation Label Lesion Magnetic Resonance Magnetic Resonance Imaging Molecular Monitor Needles Neurobiology Neurology Outcome Pathology Patients Perfusion Weighted MRI Phase I Clinical Trials Population Positioning Attribute Procedures Process Recovery Regenerative Medicine Reporting Research Research Personnel Scanning Site Specificity Spectrum Analysis Stem cells Stroke Techniques Therapeutic Time Tissue Engineering Tissues Translations Transplantation Weight abstracting base bench to bedside brain cell brain tissue cell type clinical efficacy cranium design effective therapy experience in vivo nerve stem cell non-invasive imaging non-invasive monitor pre-clinical public health relevance quantum repaired restoration success transplantation medicine

项目摘要

DESCRIPTION (provided by applicant): Non-invasive Imaging of the In Situ Restoration of Brain Tissue Abstract Regenerative medicine is gradually merging the use of stem cells and biomaterials into tissue engineering to repair damaged tissues. Developments in tissue engineering of the brain have been slow, mostly due to accessibility of the brain and being able to monitor the ongoing process non-invasively. However, non-invasive imaging, such as MRI, provides information as to the site and extent of damage in the brain, affording image- guided injection of material for tissue engineering. However, little progress has been achieved in monitoring implanted cells, as well as biomaterial non-invasively. One major challenge is to visualize these different components non-invasively without the detection of one affecting the detection of the other, or potentially the visualization of brain damage. A significant development of non-invasive imaging is therefore needed to facilitate our ability to monitor the evolution of i situ tissue engineering inside the brain. Specifically, we here aim to: 1) develop magnetic resonance imaging-based paramagnetic chemical exchange saturation transfer (PARA-CEST) to distinguish human neural stem cells (NSCs) and human endothelial cells (ECs) and 2) establish a CEST based imaging of a de-cellularized extracellular matrix (ECM) bioscaffold without interfering with our ability to detect a stroke-induced lesion cavity. These studies will provide the framework to monitor in situ tissue engineering for stroke.

描述（由申请人提供）：对脑组织的原位恢复的非侵入性成像逐渐将干细胞和生物材料的使用合并到组织工程中以修复受损的组织。大脑组织工程的发展速度很慢，主要是由于大脑的可及性并能够非侵入性地监测正在进行的过程。但是，非侵入性成像（例如MRI）提供了有关大脑损伤部位和程度的信息，可提供图像引导的组织工程材料。但是，在监测植入细胞以及生物材料的非侵入性方面几乎没有取得进展。一个主要的挑战是在不检测另一个的检测或可能可视化脑损伤的情况下，可视化这些不同的成分。一个重大的发展因此，需要非侵入性成像来促进我们监测大脑内部组织工程的演变的能力。具体而言，我们在这里的目的是：1）开发基于磁共振成像的磁共振成像化学交换饱和转移转移（PARA-CEST），以区分人类神经干细胞（NSC）和人内皮细胞（ECS）和2）建立了基于DE-Ellulardular ellarularizal化细胞外矩阵（ECM）Bioscaff的基于CEST的成像，而无需干扰我们的能力。这些研究将为监测中风的原位组织工程提供一个框架。