Non-invasive Imaging of the In Situ Restoration of Brain Tissue

脑组织原位修复的无创成像

• 批准号: 8756142
• 负责人: Michel M. Modo
• 金额: $ 29.89万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8756142
• 关键词: Address; Affect; American; Biocompatible Materials; Biological; Blood flow; Brain; Brain Injuries; Cell Fraction; Cell Therapy; Cell Transplantation; Cells; Characteristics; Chemicals; Clinical Trials; Contrast Media; Detection; Development; Diffusion; Diffusion Magnetic Resonance Imaging; Disease; Endothelial Cells; Ensure; Environment; Evolution; Extracellular Matrix; Goals; Head; Human; Image; Imagery; Imaging Techniques; Implant; In Situ; Injection of therapeutic agent; Investigation; Label; Lesion; Magnetic Resonance; Magnetic Resonance Imaging; Molecular; Monitor; Needles; Neurobiology; Neurology; Outcome; Pathology; Patients; Perfusion Weighted MRI; Phase I Clinical Trials; Population; Positioning Attribute; Procedures; Process; Recovery; Regenerative Medicine; Reporting; Research; Research Personnel; Scanning; Site; Specificity; Spectrum Analysis; Stem cells; Stroke; Techniques; Therapeutic; Time; Tissue Engineering; Tissues; Translations; Transplantation; Weight; abstracting; base; bench to bedside; brain cell; brain tissue; cell type; clinical efficacy; cranium; design; effective therapy; experience; in vivo; nerve stem cell; non-invasive imaging; non-invasive monitor; pre-clinical; public health relevance; quantum; repaired; restoration; success; transplantation medicine

DESCRIPTION (provided by applicant): Non-invasive Imaging of the In Situ Restoration of Brain Tissue Abstract Regenerative medicine is gradually merging the use of stem cells and biomaterials into tissue engineering to repair damaged tissues. Developments in tissue engineering of the brain have been slow, mostly due to accessibility of the brain and being able to monitor the ongoing process non-invasively. However, non-invasive imaging, such as MRI, provides information as to the site and extent of damage in the brain, affording image- guided injection of material for tissue engineering. However, little progress has been achieved in monitoring implanted cells, as well as biomaterial non-invasively. One major challenge is to visualize these different components non-invasively without the detection of one affecting the detection of the other, or potentially the visualization of brain damage. A significant development of non-invasive imaging is therefore needed to facilitate our ability to monitor the evolution of i situ tissue engineering inside the brain. Specifically, we here aim to: 1) develop magnetic resonance imaging-based paramagnetic chemical exchange saturation transfer (PARA-CEST) to distinguish human neural stem cells (NSCs) and human endothelial cells (ECs) and 2) establish a CEST based imaging of a de-cellularized extracellular matrix (ECM) bioscaffold without interfering with our ability to detect a stroke-induced lesion cavity. These studies will provide the framework to monitor in situ tissue engineering for stroke.

描述（由申请人提供）：脑组织原位修复的非侵入性成像 摘要再生医学正在逐渐将干细胞和生物材料的使用融入组织工程中以修复受损组织。大脑组织工程的发展一直很缓慢，主要是由于大脑的可访问性以及能够非侵入性地监测正在进行的过程。然而，非侵入性成像（例如 MRI）可提供有关大脑损伤部位和程度的信息，为组织工程提供图像引导的材料注射。然而，在非侵入性监测植入细胞以及生物材料方面取得的进展甚微。一个主要挑战是非侵入性地可视化这些不同的组件，而不会检测到其中一个组件影响另一个组件的检测，或者潜在地显示脑损伤的可视化。重大发展 因此，需要非侵入性成像来促进我们监测大脑内原位组织工程的演变的能力。具体来说，我们的目标是：1）开发基于磁共振成像的顺磁化学交换饱和转移（PARA-CEST）来区分人类神经干细胞（NSC）和人类内皮细胞（EC），2）建立基于CEST的脱细胞细胞外基质（ECM）生物支架成像，而不干扰我们检测中风诱发病变腔的能力。这些研究将为监测中风的原位组织工程提供框架。