Non-invasive Imaging of the In Situ Restoration of Brain Tissue

描述（由申请人提供）：脑组织原位修复的非侵入性成像摘要再生医学正在逐渐将干细胞和生物材料的使用融合到组织工程中以修复受损组织。大脑组织工程的发展一直很缓慢，主要是由于大脑的可访问性和能够非侵入性地监测正在进行的过程。然而，非侵入性成像，例如MRI，提供关于脑中损伤的部位和程度的信息，从而提供用于组织工程的材料的图像引导注射。然而，在非侵入性地监测植入的细胞以及生物材料方面几乎没有取得进展。一个主要的挑战是非侵入性地可视化这些不同的组件，而不影响另一个组件的检测，或者潜在地影响脑损伤的可视化。一项重大发展 因此，需要非侵入性成像来促进我们监测脑内原位组织工程的发展的能力。具体而言，我们在这里的目标是：1）开发基于磁共振成像的顺磁化学交换饱和转移（PARA-CEST），以区分人类神经干细胞（NSC）和人类内皮细胞（EC）和2）建立基于CEST的去细胞化细胞外基质（ECM）生物支架成像，而不干扰我们检测中风诱导的病变腔的能力。这些研究将提供框架，以监测原位组织工程中风。

DESCRIPTION (provided by applicant): Non-invasive Imaging of the In Situ Restoration of Brain Tissue Abstract Regenerative medicine is gradually merging the use of stem cells and biomaterials into tissue engineering to repair damaged tissues. Developments in tissue engineering of the brain have been slow, mostly due to accessibility of the brain and being able to monitor the ongoing process non-invasively. However, non-invasive imaging, such as MRI, provides information as to the site and extent of damage in the brain, affording image- guided injection of material for tissue engineering. However, little progress has been achieved in monitoring implanted cells, as well as biomaterial non-invasively. One major challenge is to visualize these different components non-invasively without the detection of one affecting the detection of the other, or potentially the visualization of brain damage. A significant development of non-invasive imaging is therefore needed to facilitate our ability to monitor the evolution of i situ tissue engineering inside the brain. Specifically, we here aim to: 1) develop magnetic resonance imaging-based paramagnetic chemical exchange saturation transfer (PARA-CEST) to distinguish human neural stem cells (NSCs) and human endothelial cells (ECs) and 2) establish a CEST based imaging of a de-cellularized extracellular matrix (ECM) bioscaffold without interfering with our ability to detect a stroke-induced lesion cavity. These studies will provide the framework to monitor in situ tissue engineering for stroke.