MR Imaging of Bioscaffold-Induced Neural Progenitor Migration

摘要 脑组织的再生一直被认为是 再生医学。植入细胞外基质(ECM)水凝胶可以 通过吸引内源性神经前体细胞进入组织来产生新生脑组织 空洞。这些神经前体起源于脑室下区(SVZ)，这是为数不多的 成人大脑中的神经性部位。最初，神经前体细胞在脑梗塞周围迁移- 在入侵生物支架之前破坏了组织。然而，人们对这条路线知之甚少 或这些细胞迁移的时间进程。这个应用程序的目标是开发一个 非侵入性磁共振成像范式，提供跟踪神经前体细胞从 SVZ到生物支架。具体地说，我们的目标是：1)开发和优化造影剂 标记内源性神经前体细胞，2)无创跟踪血管内皮细胞的迁移 神经前体细胞和3)确定神经前体细胞的时间动力学 对水凝胶植入物有反应。实现神经前体细胞的活体可视化 迁移将帮助我们了解神经发生对脑组织的贡献 不仅是为了再生，也是为了设计能够操纵这一过程的新战略。

Abstract The regeneration of brain tissue has been considered one of the greatest challenges for regenerative medicine. Implantation of an extracellular matrix (ECM) hydrogel can produce de novo brain tissue by attracting endogenous neural progenitors into a tissue cavity. These neural progenitors originate in the subventricular zone (SVZ), one of the few neurogenic sites in the adult brain. Initially, neural progenitors migrate in the peri-infarct- damaged tissue before invading the bioscaffold. However, little is known about the route or time-course of migration of these cells. The objective of this application is to develop a non-invasive MR imaging paradigm that affords the tracking of neural progenitors from the SVZ to the bioscaffold. Specifically, we here aim to: 1) develop and optimize contrast agent to label endogenous neural progenitor cells, 2) to non-invasively track the migration of neural progenitors and 3) to determine the temporal dynamics of neural progenitors responding to a hydrogel implant. Achieving an in vivo visualization of neural progenitor migrations will aid us to understand the contribution of neurogenesis to brain tissue regeneration, but also to devise novel strategies that can manipulate this process.