Long-term In Vivo Monitoring of Neuromuscular Performance in Mice

小鼠神经肌肉性能的长期体内监测

• 批准号: 8719823
• 负责人: Pedro Irazoqui
• 金额: $ 19.89万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8719823
• 关键词: Action Potentials; Amyotrophic Lateral Sclerosis; Anesthesia procedures; Animal Model; Animals; Appearance; Biocompatible; Chronic; Clinic; Collaborations; Collection; Communities; Computers; Conscious; Data; Defect; Denervation; Development; Devices; Diagnosis; Diagnostic; Disease; Disease Progression; Electromyography; Evaluation; Evaluation Studies; Event; Face; Funding; Gait; Gene Mutation; Genes; Genetic; Goals; Human; Implant; Individual; Measurement; Measures; Methods; Modeling; Molecular Genetics; Monitor; Morphologic artifacts; Motor; Motor Neurons; Movement; Mus; Muscle; Mutation; Nerve; Neuromuscular Diseases; Output; Pathology; Patients; Performance; Phenotype; Prosthesis; Protocols documentation; Reflex action; Relative (related person); Research; Research Personnel; Resolution; Superoxide Dismutase; Technology; Technology Transfer; Testing; Time; Transgenes; Universities; Validation; Wireless Technology; Work; awake; base; clinically relevant; congenic; design; electrical measurement; implantable device; improved; in vivo; meetings; mouse model; mutant; neuromuscular; novel; public health relevance; reinnervation; research and development; response; tool

DESCRIPTION (provided by applicant): Our ability to investigate the genetic basis of neuromuscular disease has entered a new era as advances in sequencing allow more rapid identification of underlying mutations. Mutations identified in patients can be moved quickly to model organisms, such as mice, for the detailed mechanistic studies not possible in patients. The number of available mouse models of neuromuscular disease is increasing constantly, as is the repertoire of available molecular genetics tools. By comparison, there is a relative lack of sophisticated tools for in vivo phenotypic characterization in mice with neuromuscular disease. In the clinic, electromyography (EMG) and nerve action potentials (NAP) data are routinely collected from patients for diagnosis and research. These measures of neuromuscular performance are particularly useful because they can be repeated and used to monitor progression or response to treatment over time. We propose to develop similar capabilities in mice by refining existing implantable technology that will allow longitudinal recording of EMG and NAP in untethered, freely moving mice.

描述（由申请人提供）：我们研究神经肌肉疾病遗传基础的能力已经进入了一个新的时代，因为测序的进步允许更快速地识别潜在的突变。 在患者中鉴定的突变可以快速转移到模型生物体，如小鼠，用于在患者中不可能进行的详细机制研究。 可用的神经肌肉疾病小鼠模型的数量不断增加，可用的分子遗传学工具库也在不断增加。 相比之下，相对缺乏用于神经肌肉疾病小鼠体内表型表征的复杂工具。 在临床上，常规地从患者收集肌电图（EMG）和神经动作电位（NAP）数据用于诊断和研究。 这些神经肌肉性能的测量特别有用，因为它们可以重复并用于监测随着时间的推移对治疗的进展或反应。 我们建议通过改进现有的可植入技术，在小鼠中开发类似的能力，该技术将允许纵向记录未拴系的自由移动小鼠的EMG和NAP。

项目成果

Enhancing the versatility of wireless biopotential acquisition for myoelectric prosthetic control.

增强肌电假肢控制无线生物电势采集的多功能性。

• DOI: 10.1088/1741-2560/13/4/046012
• 发表时间: 2016
• 期刊: Journal of neural engineering
• 影响因子: 4
• 作者: Bercich,RebeccaA;Wang,Zhi;Mei,Henry;Hammer,LaurenH;Seburn,KevinL;Hargrove,LeviJ;Irazoqui,PedroP
• 通讯作者: Irazoqui,PedroP