LRBGE Optical Microscopy Core

核心有两个目标。首先，它的使命是为受体生物学和基因表达实验室提供最先进的光学显微镜仪器。第二，核心为CCR中任何需要荧光成像协助的人员提供支持。Core目前的工作围绕着四种不同的一般技术：结构研究，如不同荧光标记的共定位；时间推移分析，以跟踪细胞成分如何随时间变化；光漂白后的荧光恢复，以测量细胞分子的迁移率和结合；荧光共振能量转移，以测量活体或固定标本中的分子相互作用。以下技术目前正在适应核心用户的需求：细胞分子扩散和结合率的数量和亮度定量和超分辨率成像（PALM/SIM/dSTORM）。该中心正在建造单分子/PALM /dSTORM显微镜，以进一步发展单分子跟踪和超分辨率分子计数。

The Core has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the Core provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the Core revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching to measure mobility and binding of cell molecules and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens. The following techniques are currently being adapted for the needs of the Core users: Number and Brightness quantification of the diffusion and binding rates of cell molecules and Super resolution imaging (PALM/SIM/dSTORM). The Core is building a Single Molecule /PALM /dSTORM microscope for the further development of single molecule tracking and molecule counting by superresolution.