LRBGE Optical Microscopy Core

核心有两个目标。首先，它的任务是为受体生物学和基因表达实验室提供最先进的光学显微镜仪器。其次，核心为CCR中任何需要荧光成像协助的人提供支持。CORE目前的工作围绕几项既定技术展开：基于高分辨率和超分辨率技术共定位不同荧光标记物的结构和形态计量学研究、追踪细胞成分随时间变化以及细胞结构、纳米机器和蛋白质如何相互作用的4D和5D时移分析、光漂白后的荧光恢复以测量细胞分子的迁移率和结合，以及荧光共振能量转移以测量活标本或固定样品中的分子相互作用。为此，核心是维护和升级高端商业显微镜。目前，Core维护或正在购买广域光学切片工作站Delta Vision Core和Delta Vision Elite、Carl Zeiss LSM780共焦显微镜、Carl Zeiss SIM/dSTORM Elyra超分辨率显微镜。核心正在采用单分子跟踪技术。正在建造一台单分子跟踪显微镜，用于同时进行多通道分子跟踪。Core正在评估卡尔·蔡司、VisiTech International和应用精密(GE Healthcare)的商用4D超分辨率仪器。CORE提交了一份定制最先进的多焦点显微镜用于4D/5D单分子跟踪的提案，这是商业上无法获得的。

The Core has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the Core provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the Core revolves around several established techniques: structural and morphometrical studies based on colocalization of different fluorescent markers by high-resolution and super-resolution techniques, 4D and 5D time-lapse analysis to follow how cellular components change over time and how cellular structures, nanomachines and proteins interact, fluorescence recovery after photobleaching to measure mobility and binding of cell molecules and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens. For that the Core is maintaining and upgrading high-end commercial microscopes. Currently the Core maintains or is in process of acquiring wide-field optical sectioning workstations Delta Vision Core and Delta Vision Elite, Carl Zeiss LSM780 confocal microscope, Carl Zeiss SIM/dSTORM Elyra Super-resolution microscope. The Core is adapting Single Molecule Tracking techniques. A Single Molecule Tracking microscope is being built for the simultaneous multichannel molecule tracking. The Core is evaluating commercial 4D super-resolution instruments from Carl Zeiss, from VisiTech International and Applied Precision (GE Healthcare). The Core submitted a proposal for custom-building of the state-of-the art multifocus microscope for 4D/5D single molecule tracking, not available commercially.