LRBGE Optical Microscopy Core

核心任务是为CCR和NIH中任何需要荧光成像协助的人提供最先进的光学显微镜仪器。CORE目前的工作围绕几项既定技术展开：基于高分辨率和超分辨率技术共定位不同荧光标记物的结构和形态计量学研究、追踪细胞成分随时间变化以及细胞结构、纳米机器和蛋白质如何相互作用的4D和5D时移分析、光漂白后的荧光恢复以测量细胞分子的迁移率和结合，以及荧光共振能量转移以测量活标本或固定样品中的分子相互作用。为此，核心是维护和升级高端商业显微镜。目前，Core拥有广域光学切片显微镜Delta Vision Elite、卡尔·蔡司LSM780和880共焦显微镜和晶格光片3I显微镜，以及卡尔·蔡司SIM/dSTORM Elyra超分辨率显微镜、MINFLUX显微镜。Core利用定制的HELO显微镜和MINFLUX开发单分子跟踪技术。该系统采用MINFLUX纳米分辨率技术来绘制细胞中蛋白质的分子图谱。核心不断调查对最先进技术的需求，为OMC用户测试和安排新仪器的演示，并宣传可能对当前和潜在客户的特定需求有用的新显微镜方法。

The Core mission is to provide state-of-the-art light microscopy instrumentation to anyone in the CCR and NIH who requires assistance with fluorescence imaging. Present work in the Core revolves around several established techniques: structural and morphometrical studies based on colocalization of different fluorescent markers by high-resolution and super-resolution techniques, 4D and 5D time-lapse analysis to follow how cellular components change over time and how cellular structures, nanomachines and proteins interact, fluorescence recovery after photobleaching to measure mobility and binding of cell molecules and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens. For that the Core is maintaining and upgrading high-end commercial microscopes. Currently the Core maintains wide-field optical sectioning microscope Delta Vision Elite, Carl Zeiss LSM780 and 880 confocal microscopes and Lattice Light Sheet 3I microscope, and Carl Zeiss SIM/dSTORM Elyra Super-resolution microscope, MINFLUX microscope. The Core develops Single Molecule Tracking technique, utilizing custom-built HILO microscopes and MINFLUX. The system adapts MINFLUX nano-resolution technique for molecular maps of proteins in cells. The Core constantly surveys the demands for the state-of-the art technology, tests and arranges demonstrations of the new instruments for OMC users and advertises new microscopy methods that may be useful for specific needs of the current and prospective customers.