Epigenetic Biomarker Discovery in HPV related HNSCC

描述（由申请人提供）：在美国，高危人乳头瘤病毒相关的头颈部鳞状细胞癌（HPVHNSCC）的发病率正在迅速上升，并将很快超过宫颈癌的发病率。目前还没有有效的方法来检测临床前或早期HPVHNSCC，筛查HPVHNSCC发展风险增加的人群，甚至在治疗后检测HPVHNSCC的显微镜持久性。然而，在恶性前病变和早期癌症患者的体液中检测到表观遗传改变，多重检测显示出识别口腔恶性前病变患者的希望。我们的主要假设是，基于HPVHNSCC特异性表观遗传改变和高风险HPV DNA存在的血浆和唾液冲洗液的多重检测有可能作为HPVHNSCC存在的有用检测。该项目将作为HPVHNSCC检测的发现和诊断验证生物标志物开发试验，潜在应用于评估疾病反应和开发基于人群的筛查试验。

DESCRIPTION (provided by applicant): High risk human papilloma virus related head and neck squamous cell carcinoma (HPVHNSCC) incidence is rapidly increasing, and will soon surpass the incidence of cervical carcinoma in the US. There are no effective ways to detect preclinical or early HPVHNSCC, screen populations at increased risk for HPVHNSCC development, or even assay for microscopic persistence of HPVHNSCC after therapy. However, epigenetic alterations have been detected in body fluids from premalignant and early stage cancers and multiplex tests have shown promise for identification of patients with oral premalignancy. Our primary hypothesis is that multiplex testing of plasma and salivary rinses based on HPVHNSCC specific epigenetic alterations combined with high risk HPV DNA presence has potential as useful test for presence of HPVHNSCC. This project will serve as a discovery and diagnostic validation biomarker development trial for HPVHNSCC detection with potential application for assessment of disease response and development of a population based screening test.