Ultrasound-aided gene transfer to direct cortical neurogenesis after brain injury
超声辅助基因转移至脑损伤后直接皮质神经发生
基本信息
- 批准号:8722168
- 负责人:
- 金额:$ 40.49万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-02-15 至 2019-03-31
- 项目状态:已结题
- 来源:
- 关键词:AdultAdverse effectsAnimal ModelBehavioralBiological AssayBioluminescenceBlood PressureBrainBrain EdemaBrain InjuriesCause of DeathCell ProliferationCellsChargeChildClinical TrialsCognitiveDevelopmentDrug FormulationsElderlyEmotionalEnzyme-Linked Immunosorbent AssayEpendymal CellEvaluationFibroblast Growth FactorFibroblast Growth Factor 2Focused Ultrasound TherapyFrequenciesGene DeliveryGene TransferGenesGoalsHealedHemolysisHistologyImageImmunofluorescence ImmunologicImmunohistochemistryInjuryInterventionIntracranial PressureIntraventricular InjectionsKineticsLabelLeadLesionLipidsMagnetic Resonance ImagingMammalsMediatingMedicalMembraneMethodsMicrobubblesModelingMonitorMotorNeurogliaNeuronal DifferentiationNeuronsNucleic AcidsOutcomePathway interactionsPatientsPeptidesPolymersProductionProteinsRattusRecoveryReporter GenesRiskRodent ModelSiteSpecificityStem cellsStreamStructure of choroid plexusSurfaceSystemTechnologyTestingTherapeuticTransfectionTraumatic Brain InjuryUltrasonographyUnited StatesWorkcell typecognitive recoverydisabilityfallsfunctional improvementhealinghigh riskimprovedimproved functioningin vitro Modelin vitro testingin vivoinnovationlight scatteringmigrationmotor vehicle injurynerve stem cellneurogenesispolymerizationpublic health relevancerelating to nervous systemrepairedresponsestemsubventricular zonetherapeutic genetranscription factortransgene expressionvectoryoung adult
项目摘要
DESCRIPTION: Traumatic brain injury (TBI), commonly caused by motor vehicle injury and falls in the young and elderly, afflicts nearly 1.7 million people in the United States each year. n addition to being a leading cause of death in children and young adults, TBI is also a major cause of permanent disability in the United States. There are currently no curative treatments for TBI, and the main course of action is to minimize secondary damage that results from changes in blood pressure, brain swelling, or intracranial pressure that is triggered by the initia injury. The overall goal of this project is to first develop a well-tolerated means of transferring
genes to neural progenitor cells in the brain and then to use this technology to direct cortical neurogenesis after injury. We propose to use focused ultrasound to enhance non-viral gene transfer to neural progenitor cells in the brain mediated by a targeted polymer delivery vector, thus enabling the delivery of genes encoding fibroblast growth factor-2 and neurogenin2, proteins shown to enhance neurogenesis and direct neuron differentiation, respectively. We will evaluate in an animal model of TBI whether induced migration and increased neuronal integration can lead to functional improvement through a combination of histology, cognitive evaluations, and motor assessments. .
产品说明:创伤性脑损伤(TBI),通常由机动车损伤和年轻人和老年人的福尔斯跌倒引起,每年折磨着美国近170万人。除了是儿童和年轻人死亡的主要原因外,TBI也是美国永久性残疾的主要原因。目前还没有针对TBI的治愈性治疗,并且主要的行动过程是最小化由初始损伤触发的血压、脑肿胀或颅内压变化引起的继发性损伤。该项目的总体目标是首先开发一种耐受性良好的转移手段,
基因到大脑中的神经祖细胞,然后使用这种技术来指导受伤后的皮层神经发生。我们建议使用聚焦超声,以提高非病毒基因转移到神经祖细胞在大脑中介导的有针对性的聚合物传递载体,从而使编码成纤维细胞生长因子-2和neurogenin 2,蛋白质,以增强神经发生和直接神经元分化,分别交付的基因。我们将通过组织学、认知评估和运动评估的组合,在TBI动物模型中评估诱导的迁移和增加的神经元整合是否可以导致功能改善。.
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Philip J Horner其他文献
Philip J Horner的其他文献
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{{ truncateString('Philip J Horner', 18)}}的其他基金
Spinal Neuromodulation to Promote Physiologic and Molecular Plasticity in theInjured Spinal Cord
脊髓神经调节促进受损脊髓的生理和分子可塑性
- 批准号:
10805726 - 财政年份:2023
- 资助金额:
$ 40.49万 - 项目类别:
Training in Neural Control of organ Degeneration and Regeneration (NeuralCODR)
器官退化和再生的神经控制培训(NeuralCODR)
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10620833 - 财政年份:2022
- 资助金额:
$ 40.49万 - 项目类别:
Patricia Levy Zusman International Workshop on Neuroregeneration (Zusman Workshop)
Patricia Levy Zusman 神经再生国际研讨会(Zusman 研讨会)
- 批准号:
10607404 - 财政年份:2022
- 资助金额:
$ 40.49万 - 项目类别:
Training in Neural Control of organ Degeneration and Regeneration (NeuralCODR)
器官退化和再生的神经控制培训(NeuralCODR)
- 批准号:
10410250 - 财政年份:2022
- 资助金额:
$ 40.49万 - 项目类别:
A versatile reporter for visualization of myelin plasticity in the genetically modified rat
一种多功能报告基因,用于可视化转基因大鼠的髓磷脂可塑性
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10303241 - 财政年份:2021
- 资助金额:
$ 40.49万 - 项目类别:
CNS Neuroregeneration strategies: Discovery and Implementation
中枢神经系统神经再生策略:发现和实施
- 批准号:
9332048 - 财政年份:2017
- 资助金额:
$ 40.49万 - 项目类别:
Astrocyte-specific ligand discovery by phage display
通过噬菌体展示发现星形胶质细胞特异性配体
- 批准号:
8995701 - 财政年份:2015
- 资助金额:
$ 40.49万 - 项目类别:
Metabolic requirements of adult neural stem cells
成体神经干细胞的代谢需求
- 批准号:
8068094 - 财政年份:2011
- 资助金额:
$ 40.49万 - 项目类别:
Metabolic requirements of adult neural stem cells
成体神经干细胞的代谢需求
- 批准号:
8321500 - 财政年份:2011
- 资助金额:
$ 40.49万 - 项目类别:
Combined stem cell transplantation and targeted microstimulation to direct the fo
联合干细胞移植和定向微刺激来指导
- 批准号:
8288742 - 财政年份:2009
- 资助金额:
$ 40.49万 - 项目类别:
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