STRUCTURAL AND BIOCHEMICAL STUDIES OF PROTEIN TYROSINE PHOSPHATASE FUNCTION

蛋白质酪氨酸磷酸酶功能的结构和生化研究

• 批准号: 8732670
• 负责人: Samuel Bouyain
• 金额: $ 23.76万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8732670
• 关键词: Accounting; Adhesives; Adult; Binding; Biochemical; Biological Neural Networks; Cell Adhesion; Cell Adhesion Molecules; Cell Surface Receptors; Cell surface; Cell-Cell Adhesion; Cells; Complex; Cues; Data; Development; Dimerization; Embryonic Development; Epitopes; Equilibrium; Event; Extracellular Domain; Extracellular Matrix; Extracellular Protein; Family; Foundations; Goals; Growth Factor; Ligand Binding; Ligands; Link; Maintenance; Mediating; Mission; Molecular; Nervous system structure; Neurites; Neuroglia; Neurons; Orphan; PTPRG gene; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphotyrosine; Play; Process; Protein Dephosphorylation; Protein Tyrosine Kinase; Protein Tyrosine Phosphatase; Proteins; Public Health; Research; Role; Signal Transduction; Specificity; Sterile coverings; Structure; Tenascin; Tissues; Tweens; Tyrosine; Tyrosine Phosphorylation; United States National Institutes of Health; War; Work; base; contactin; design; extracellular; grasp; human PTPRT protein; insight; member; nervous system development; neurodevelopment; pleiotrophin; receptor; relating to nervous system

DESCRIPTION (provided by applicant): It has long been appreciated that receptor protein tyrosine phosphatases (RPTPs) play key roles in cell adhesion and cell signaling. However, the lack of information on their extracellular binding partners and on the functional link between cell adhesion and intracellular phosphatase activity is currently a substantial gap in our understanding of how these receptors mediate the extracellular binding and tyrosine dephosphorylation events necessary for the development of neural tissues. Among RPTPs, PTPRZ (RPTP2/PTP6) is expressed predominantly on glial cells and has been associated with multiple binding partners such as tenascin-C in the extracellular matrix and contactin1 on neurons. Interactions between PTPRZ and contactin1 induce neurite outgrowth and reduce phosphotyrosine levels in cells expressing PTPRZ. Interestingly, the interactions between PTPRZ and contactin1 are impaired in the presence of tenascin-C, revealing the presence of an intricate interplay between these proteins and the interactions between neurons and glial cells. However, the structural basis for these interactions remains unclear. Our long-term goal of is to dissect the mechanisms of cell adhesion and cell signaling that underlie the construction of neural networks. The objective of this proposal is to provide a structural basis for the binding of PTPRZ to its binding partners, which is a prerequisite first step towards defining the function of the cell adhesion complexes involving PTPRZ. The rationale for the research proposed here is that structural characterizations of receptor-ligand pairs involving PTPRZ would represent a significant progress towards understanding RPTP-mediated cell adhesion. Our preliminary data demonstrate the feasibility of our structural approach and we propose the following specific aims to fulfill the need for a detailed understanding of the structural aspects of RPTP-mediated cell adhesion: (1) to determine the crystal structure of the complex between PTPRZ and contactin1 to provide a structural basis for the adhesive interactions mediated by these two proteins, (2) to analyze the effect of contactin1 binding on the oligomeric state of PTPRZ and its intracellular phosphatase activity and (3) to establish the mechanism by which tenascin-C interferes with the binding between PTPRZ and CNTN1 by determining the crystal structures of PTPRZ-tenascin-C and contactin1-tenascin-C complexes. Our contribution is significant because we will be able to visualize protein interfaces in cell adhesion complexes involving RPTPs, yielding precious information about ligand-receptor specificity as well as potential conformational changes and/or changes in oligomeric states that occur upon ligand binding. These structural insights will lay the foundations upon which biochemical studies of RPTP function will be built. Overall, these studies will be relevant to the mission of NIH because they will provide a clearer molecular picture of the cell adhesion events that underpin the development and maintenance of the nervous system and ultimately illuminate the relationship between cell adhesion and cell signaling mediated by RPTPs.

描述（由申请人提供）：

项目成果

Receptor-type tyrosine phosphatase ligands: looking for the needle in the haystack.

• DOI: 10.1111/j.1742-4658.2012.08653.x
• 发表时间: 2013-01
• 期刊: The FEBS journal
• 作者: Mohebiany AN;Nikolaienko RM;Bouyain S;Harroch S
• 通讯作者: Harroch S

The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation.

蛋白酪氨酸磷酸酶 LAR 的免疫球蛋白样结构域 1 和 2 采用不寻常的马蹄形构象。

• DOI: 10.1016/j.jmb.2011.03.013
• 发表时间: 2011-05-13
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Biersmith BH;Hammel M;Geisbrecht ER;Bouyain S
• 通讯作者: Bouyain S

Noncanonical FK506-binding protein BDBT binds DBT to enhance its circadian function and forms foci at night.

• DOI: 10.1016/j.neuron.2013.08.004
• 发表时间: 2013-11-20
• 期刊: Neuron
• 影响因子: 16.2
• 作者: Fan JY;Agyekum B;Venkatesan A;Hall DR;Keightley A;Bjes ES;Bouyain S;Price JL
• 通讯作者: Price JL

Contactins: structural aspects in relation to developmental functions in brain disease.

Contactins：与脑部疾病发育功能相关的结构方面。

• DOI: 10.1016/b978-0-12-386483-3.00001-x
• 发表时间: 2011
• 期刊: Advances in protein chemistry and structural biology
• 作者: Zuko,Amila;Bouyain,Samuel;vanderZwaag,Bert;Burbach,JPeterH
• 通讯作者: Burbach,JPeterH

Structural Basis for Interactions Between Contactin Family Members and Protein-tyrosine Phosphatase Receptor Type G in Neural Tissues.

Contactin 家族成员与神经组织中 G 型蛋白酪氨酸磷酸酶受体之间相互作用的结构基础。

• DOI: 10.1074/jbc.m116.742163
• 发表时间: 2016
• 期刊: The Journal of biological chemistry
• 作者: Nikolaienko,RomanM;Hammel,Michal;Dubreuil,Véronique;Zalmai,Rana;Hall,DavidR;Mehzabeen,Nurjahan;Karuppan,SebastianJ;Harroch,Sheila;Stella,SalvatoreL;Bouyain,Samuel
• 通讯作者: Bouyain,Samuel