Autoclave Improvements for UA Animal Facilities

UA 动物设施的高压灭菌器改进

• 批准号: 8712089
• 负责人: DAVID G BESSELSEN
• 金额: $ 47.89万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712089
• 关键词: Accounting; Address; Affect; Aging; American; Animal Experimentation; Animals; Area; Arizona; Arthritis; Award; Behavior; Biomedical Engineering; Cessation of life; Development; Diabetes Mellitus; Disease; Engineering; Equipment and supply inventories; Faculty; Frequencies; Funding; Future; Genetically Engineered Mouse; Genomics; Goals; Grant; Health; Health Sciences; Healthy People 2020; Heart Diseases; Immune; Individual; Infectious Disease Immunology; Injury; Life; Lung diseases; Maintenance; Malignant Neoplasms; Memory; Mus; Natural Resources; Pain; Personal Satisfaction; Physical environment; Population; Preventive; Principal Investigator; Quality of life; Quarantine; Research; Rodent; Services; Social Environment; Source; Staging; Sterility; Sterilization; Universities; Water; animal facility; disability; graduate student; improved; operation; premature; programs; repaired

SPECIFIC AIMS Research programs at the University of Arizona (UA) focus on the goals of the DHHS Healthy People 2020 initiative: attain high-quality, longer lives free of preventable disease, disability, injury, and premature death; eliminate disparities and improve the health of all groups; create social and physical environments that promote good health for all; and promote quality of life, healthy development, and healthy behaviors across all life stages. Specific areas of UA research excellence include infectious disease, immunology, cancer, heart disease, arthritis, diabetes, respiratory disease, memory and aging, pain alleviation, genomics, and biomedical engineering. Animal research is vital to these and other research programs that are growing at the UA. Animal usage has increased 30% over the past three years, and research funding and animal use is projected to at least double between now and 2020. Genetically engineered mice (GEM) and rodents used in research conducted at Animal Biosafety Level 2 (ABSL-2) are responsible for recent expansion, and will account for the vast majority of future expansion. Autoclaves are heavily relied upon to sterilize caging for GEM and to decontaminate ABSL-2 rodent caging. Autoclave reliability is a major programmatic concern, and increased autoclave capacity is needed to accommodate the rapidly expanding animal inventory at the UA. This project directly addresses these needs through replacement, addition, or upgrades of autoclaves in the three largest centralized animal facilities at the UA, affecting a total of 66,237 nasf space and impacting 106 PHS-funded grants awarded to 78 Principal Investigators with $46.8 million total annual funding. The project will vastly improve reliability, increase capacity, and provide redundancy of autoclaves in the facilities that maintain large rodent populations in sterile and ABSL-2 caging. The project will also significantly improve energy efficiency and preserve natural resources, particularly water. Improved animal facilities will aid in recruitment of research faculty, graduate students, and staff. Resulting research discoveries will enhance the health and well-being of all Americans. Specific Aim 1: Replace a 48 year old autoclave at the Arizona Health Sciences Center Animal Facility (AHSC) (47,000 gsf; 28,325 nasf). This is one of two large autoclaves located in the cage wash area that serves the largest UA animal facility and is at the end of its useful life. It is the primary autoclave used to sterilize rodent caging for GEM and immune deficient rodents for this facility. The repair frequency for this autoclave is high and parts have become extremely difficult to obtain. Specific Aim 2: Install a second autoclave at the Central Animal Facility (CAF) (40,254 gsf; 21,139 nasf). The only autoclave located in the CAF cage wash area is 25 years old and nearing the end of its useful life. It is used to sterilize rodent caging for GEM and to decontaminate non-approved source rodent quarantine and ABSL-2 rodent caging. The repair frequency for this autoclave is high, parts have become extremely difficult to obtain, one door is no longer functional, and prolonged downtime requires caging be transported one mile to and from the AHSC animal facility for sterilization. Installing a second autoclave provides redundancy in the near term and a replacement when the existing autoclave is no longer repairable. Specific Aim 3: Upgrade control units and door gaskets on two autoclaves in the BIO5 Animal Facility (BIO5) (23,040 gsf; 16,773 nasf). These two autoclaves serve the entire GEM barrier facility where only sterile mouse caging is used. Use of these autoclaves is frequently disrupted due to poorly engineered, proprietary control units that are not easily repaired, and door gaskets that must be removed and serviced at least once every 3 weeks. Together, these improvements will greatly enhance autoclave reliability, increase autoclave capacity needed for expansion, and provide redundancy to allow continued operations during preventive maintenance or repair of individual autoclaves at each of the three largest UA animal facilities.

具体目标 亚利桑那大学（UA）的研究项目专注于DHHS健康人2020的目标 倡议：实现高质量、更长的寿命，避免可预防的疾病、残疾、伤害和过早死亡; 消除差距，改善所有群体的健康;创造社会和物质环境， 促进所有人的健康;促进所有人的生活质量、健康发展和健康行为 生命阶段UA研究卓越的具体领域包括传染病，免疫学，癌症，心脏病， 疾病，关节炎，糖尿病，呼吸系统疾病，记忆和衰老，缓解疼痛，基因组学和生物医学 工程.动物研究对这些和其他在美国发展的研究项目至关重要。 在过去的三年里，动物的使用增加了30%，研究资金和动物的使用预计 到2020年至少翻一番用于研究的基因工程小鼠和啮齿动物 在动物生物安全2级（ABSL-2）进行，负责最近的扩展，并将占 绝大多数未来的扩张。严重依赖高压灭菌器对GEM的笼舍进行灭菌， 净化ABSL-2啮齿动物笼。高压灭菌器的可靠性是一个主要的计划问题， 需要高压灭菌器的容量来适应UA快速扩大的动物库存。这个项目 通过更换、增加或升级三个最大的国家和地区的高压灭菌器， 美国的集中动物设施，影响了66,237个nasf空间，影响了106个公共卫生系统资助的 向78名首席调查员提供赠款，年度资金总额为4 680万美元。该项目将大大 提高可靠性，增加容量，并在保持大容量的设施中提供高压灭菌器的冗余 无菌和ABSL-2笼中的啮齿动物种群。该项目还将显著提高能源效率 保护自然资源，特别是水资源。改善动物设施将有助于招募研究人员 教师，研究生和工作人员。由此产生的研究发现将提高健康和福祉， 都是美国人 具体目标1：更换亚利桑那州健康科学中心动物设施的48岁高压灭菌器 （AHSC）（47，000 gsf; 28，325 nasf）。这是位于笼清洗区的两个大型高压灭菌器之一， 服务于最大的UA动物设施，并处于其使用寿命的终点。它是一级高压灭菌器， 用于GEM的啮齿动物笼和用于该设施的免疫缺陷啮齿动物的灭菌啮齿动物笼。维修频率 高压釜的压力很高，部件变得非常难以获得。 具体目标2：在中央动物机构（CAF）安装第二台高压灭菌器（40，254 gsf; 21，139 nasf）。的 只有位于CAF笼清洗区的高压灭菌器使用了25年，接近其使用寿命。是 用于对GEM的啮齿动物笼进行灭菌，并对未经批准的来源啮齿动物检疫进行去污， ABSL-2啮齿动物笼舍。这种高压灭菌器的维修频率很高，零件变得非常困难， 获得，一个门不再起作用，长时间的停机时间需要将笼子运输一英里， 和AHSC动物设施进行灭菌。安装第二个高压灭菌器可提供冗余， 短期内和更换时，现有的高压灭菌器不再是可修复的。 具体目标3：升级BIO 5动物设施（BIO 5）中两台高压灭菌器的控制单元和门垫圈 (23 040 gsf; 16，773 nasf）。这两个高压灭菌器用于整个GEM屏障设施，其中仅无菌小鼠 使用了屏蔽。这些高压灭菌器的使用经常中断，由于工程不良，专有控制 不容易修理的单元，以及必须至少每3个小时拆卸和维修一次的门垫圈 周 总之，这些改进将大大提高高压灭菌器的可靠性，增加高压灭菌器所需的容量， 用于扩展，并提供冗余，以便在预防性维护或维修期间继续运行 美国三个最大的动物设施中的每一个的单独高压灭菌器。