CORE--EXPERIMENTAL ANIMAL FACILITY

核心——实验动物设施

• 批准号: 6563871
• 负责人: DAVID G BESSELSEN
• 金额: $ 13.05万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563871
• 关键词: biomedical facility; gene targeting; genetically modified animals; laboratory mouse

APPLICANT'S DESCRIPTION: The goal of the experimental animal core is to provide relevant mouse models to study colon tumor development and to study apoptosis in colon carcinogenesis. The mouse models will be used to evaluate genetic and extracellular factors (dietary polyamines, bile acids) that affect the apoptotic pathway and to evaluate the effects of potential chemopreventive agents. Included in these models are mice that have defective APC, p53, and NOS-2 genes, genes that have been shown or are hypothesized to be involved in the oncogenesis of colon tumors in both humans and mice. These models will allow further clarification of the biochemical mechanisms involved in colon carcinogenesis and will identify additional molecular targets for chemoprevention. The specific aims of the core are: 1) to provide relevant murine models to study colon oncogenesis and the apoptotic pathway; 2) to provide treatments, carcinogens, and dietary supplements that promote tumor development in each appropriate model; 3) to administer chemopreventive agents; 4) to provide fresh tissues from the animal study protocols to each project; and 5) to perform gross and histologic analyses of intestinal tumors that develop in the murine models. The experimental animal core provides a means by which animals needed for studies in three different projects can be shared and the results of the studies are combinative. The advantages to having a experimental animal core are: less animals are needed than if each project performed the animal studies by themselves; there is uniformity and quality control in carrying out the study protocols across each project; data generated from each study is comparable given that the information is generated from the same sources with common controls; and the core allows examination of multiple rodent models of colon cancer that would otherwise be unaffordable or unavailable.

申请人描述：实验动物核心的目标是 提供相关的小鼠模型来研究结肠肿瘤的发展和研究 结肠癌发生过程中的细胞凋亡。小鼠模型将用于评估 影响的遗传和细胞外因素（膳食多胺、胆汁酸） 细胞凋亡途径并评估潜在化学预防的效果 代理。这些模型中包括具有 APC、p53 缺陷和 NOS-2 基因，已显示或假设参与的基因 人类和小鼠结肠肿瘤的发生。这些模型将 可以进一步阐明结肠中涉及的生化机制 致癌作用并将确定其他分子靶标 化学预防。核心的具体目标是：1）提供相关的 研究结肠肿瘤发生和细胞凋亡途径的小鼠模型； 2）到 提供促进肿瘤生长的治疗方法、致癌物质和膳食补充剂 开发每个适当的模型； 3) 化学预防 代理； 4) 向每个动物提供来自动物研究方案的新鲜组织 项目; 5) 对肠道肿瘤进行大体和组织学分析 在小鼠模型中发育。实验动物核心提供了 三个不同项目研究所需的动物可以通过这些方式 共享并且研究结果是组合的。优点 拥有实验动物核心是：比每个实验动物所需的动物更少 项目自行进行动物研究；存在统一性并且 在每个项目中执行研究方案的质量控制；数据 鉴于信息是每项研究生成的，因此具有可比性 由具有共同控制的相同来源生成；并且核心允许 检查多种结肠癌啮齿动物模型，否则这些模型将被 负担不起或无法获得。