Architectures for the macromolecule-mediated assembly of palindromic rotaxanes

回文轮烷大分子介导的组装结构

• 批准号: 8643492
• 负责人: Leah S Witus
• 金额: $ 5.15万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-05 至 2016-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8643492
• 关键词: Architecture; Area; Biological; Biomimetics; DNA; Development; Devices; Fiber; Gel; Hydrogels; Individual; Lateral; Mediating; Methods; Molecular; Molecular Machines; Molecular Motors; Motion; Motor; Muscle; Muscle Contraction; Nature; Operative Surgical Procedures; Peptides; Polymers; Property; Prosthesis; Proteins; Research; Rice; Rotaxanes; Scheme; Stimulus; Structure; Training; Translating; Universities; crosslink; design; experience; graduate student; macromolecule; minimally invasive; molecular scale; nanorobotics; oxidation; public health relevance; robotic device; self assembly; tool

DESCRIPTION (provided by applicant): This proposal is on the macromolecule-mediated assembly of rotaxanes to access higher order organizations of individual molecular motors. Doubly bistable palindromic rotaxanes, a type of mechanically interlocked molecule developed in the Stoddart lab, are capable of switchable and reversible molecular motions that mimic muscular contraction. To achieve macroscale effects from these molecular motions for applications such as artificial muscles and biomedical devices, organized assemblies are required. Three types of macromolecules, DNA, peptides and polymers, will be used to assemble the rotaxanes. It will be possible to access these three distinct architectures by functionalizing the rotaxane structures with the respective macromolecules post-synthesis. The DNA- and peptide-rotaxane conjugates will utilize the known properties of biomolecule self-assembly to form linear rotaxane oligomers. The rotaxane-polymer conjugate will be used to create a rotaxane-crosslinked hydrogel in order to translate the molecular scale motions of the bistable rotaxane to macroscopic effects via a size change of the gel. These assemblies will coordinate the individual molecular motions of the rotaxanes and enable their use in the creation of artificial muscle materials.

描述（由申请人提供）：该提案是关于轮烷的大分子介导的组装，以获得单个分子马达的更高级组织。双重回文轮烷是Stoddart实验室开发的一种机械联锁分子，能够模拟肌肉收缩的可切换和可逆的分子运动。为了从这些分子运动中获得宏观尺度效应，例如人造肌肉和生物医学设备，需要有组织的组装。三种类型的大分子，DNA，肽和聚合物，将用于组装轮烷。通过在合成后用相应的大分子官能化轮烷结构，将有可能获得这三种不同的结构。DNA-和肽-轮烷缀合物将利用生物分子自组装的已知性质形成线性轮烷低聚物。轮烷-聚合物缀合物将用于产生轮烷交联的水凝胶，以便通过凝胶的尺寸变化将轮烷的分子尺度运动转化为宏观效应。这些组件将协调轮烷的单个分子运动，并使其能够用于制造人工肌肉材料。