Nonhuman Primate IVIS Spectrum Imaging System

描述(由申请人提供)：加州国家灵长类动物研究中心活体成像翻译计划是计算成像核心的一个组成部分，为美国国立卫生研究院资助的全国研究人员提供最先进的活体成像服务和专业知识。这一既定的计划包括为研究人员提供独特的机会，将非侵入性成像纳入他们的研究计划，并研究人类健康和疾病的猴子模型。这项提议的目标是升级和更换制造商/供应商将不再支持或维修的光学成像系统，使许多正在进行和计划中的NIH研究面临风险。替换的IVIS光谱成像系统将在这一已建立的核心服务结构内维护，该结构在满足美国国立卫生研究院支持的全国研究人员的体内成像需求方面一直非常有效。该成像系统将安装在安全环境中的专用成像套件中，并且具备用于研究非人类灵长类动物的职业健康和安全所需的条件。IVIS光谱成像系统既可用于反射模式，也可用于传输模式，它包括一个高灵敏度的背面薄、背面照明的CCD相机、一个带有荧光测量过滤器的宽带激发源、用于多光谱检测的可选过滤器，以及用于控制数据采集、光谱解混、3D信号定位和量化以及图像显示和分析的软件。除了目前在猴子身上进行的专注于基因转移/基因治疗、干细胞移植、再生医学、组织工程、传染病和寿命健康的纵向研究外，该系统还将成为这些新成像技术在猴子身上进一步开发和应用的平台，并跨越研究领域。这一替代系统将确保加州国家灵长类研究中心正在进行的和新的研究机会能够继续满足NIH资助的研究人员的需求，这些研究人员专注于更好地了解人类健康和疾病的方法。

DESCRIPTION (provided by applicant): The California National Primate Research Center translational in vivo imaging program, which is a component of the Computational Imaging Core, provides state-of-the-art in vivo imaging services and expertise to NIH- funded investigators nationally. This established program includes unique opportunities for investigators to incorporate noninvasive imaging in their research programs, and to study monkey models of human health and disease. The goal of this proposal is to upgrade and replace an optical imaging system that will no longer be supported or serviced by the manufacturer/supplier placing many NIH studies in progress and planned at risk. The replacement IVIS Spectrum Imaging System will be maintained within this established core service structure, which has been highly effective in meeting the in vivo imaging needs of NIH-supported investigators nationwide. This imaging system will be located within a dedicated imaging suite in a secure environment, and with the required conditions for occupational health and safety for research with nonhuman primates. The IVIS Spectrum Imaging System can be used in both reflectance and transmission modes and includes a highly sensitive back-thinned, back-illuminated CCD camera, a broad band excitation source with filters for fluorescence measurements, selectable filters for multispectral detection, and software to control data acquisition, for spectral unmixin, 3D signal localization and quantification, and image display and analysis. In addition to ongoing longitudinal studies in monkeys focused on gene transfer/gene therapy, stem cell transplantation, regenerative medicine, tissue engineering, infectious diseases, and lifespan health, the system will serve as the platform for further development and application of these novel imaging techniques in monkeys, and span research domains. This replacement system will ensure ongoing and new research opportunities at the California National Primate Research Center can continue to meet the needs of NIH- funded investigators locally, regionally, and nationally that are focused on ways to better understand human health and disease.