Osteogenic Calcium Phosphate Nanoparticles with Designable Drug Release Kinetics

具有可设计药物释放动力学的成骨磷酸钙纳米颗粒

• 批准号: 8853384
• 负责人: Vuk Uskokovic
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-05 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8853384
• 关键词: Address; Antibiotics; Antifungal Agents; Area; Award; Biocompatible Materials; Biodegradation; Biological; Biomaterials Research; Biomedical Engineering; Cell Culture Techniques; Chemicals; Clindamycin; Complement; Consult; Defect; Dental; Dental Schools; Drug Compounding; Drug Controls; Drug Delivery Systems; Drug usage; Emulsions; Encapsulated; Engineering; Fibroblasts; Filler; Fluorescent Dyes; Funding; Goals; Grant; Growth Factor; Health; Hemostatic Agents; Image; In Vitro; Institutes; Intravenous; K-Series Research Career Programs; Kinetics; Knowledge; Lead; Marshal; Mentors; Methods; Morphology; Natural regeneration; Oral; Osteoblasts; Osteomyelitis; Particle Size; Pathway interactions; Performance; Pharmaceutical Preparations; Phase; Polymers; Precipitation; Procedures; Property; Protocols documentation; Research; Research Activity; Research Training; Science; Solutions; Strategic Planning; Suspension substance; Suspensions; Testing; Therapeutic; Time; Tissues; Training; Training Activity; Training Programs; Trauma; United States National Institutes of Health; Work; Writing; antimicrobial; base; bone; calcium phosphate; craniofacial; design; drug production; implantation; nanoparticle; nanosized; nanostructured; osteogenic; particle; response; skills; stoichiometry; theranostics

This K99/R00 Mentored Research Career Development Award application is being submitted to the National Institute on Dental and Craniofacial Research (NIDCR). The candidate, Dr. Vuk Uskokovic, is a physical chemist and nanotechnologist who seeks to obtain additional training in the area of cell culture experimentation and other biological methods of analysis through this award. His aim is to develop research skills in the area of multifunctional and nanostructured biomaterials for reparation of hard tissues. The goals of this proposal would enhance Dr. Uskokovic's knowledge of fabrication of smart biomaterials for simultaneous time-controlled drug release and regeneration of diseased hard tissues. Proposed is the synthesis of calcium phosphate/polymer composite particles that encapsulate clinmadycin, a drug used in the treatment of osteomyelitis, as well as testing of the antimicrobial properties and osteinductive performance of the given material in vitro. The central hypothesis of the proposed study is that stoichiometry and the particle size of calcium phosphates can be used to tune the kinetics of the release of the encapsulated drug. Dr. Uskokovic will conduct his training and research activities under the guidance of an expert mentoring team that includes Drs. Tejal Desai, Grayson Marshall, Stefan Habelitz, Wu Li, and Peter Loomer. He will receive additional consulting from Drs. Mauro Ferrari, Antoni Tomsia, and Charles Hoover. Given that bioengineering and biomaterials research approaches to regeneration of oral and craniofacial tissues has been selected as one of the prioritized goals by the NIDCR (Objective I-5, Goal 1, NIDCR Strategic Plan 2009-2013), the goals of the current application will address highly actual oral, dental and craniofacial health problems. Training Dr. Uskokovic through this Pathway to Independence Award on top of his expertise in materials science and engineering and support by the UCSF Dental School, the most funded one by the NIH in the past 15 years, also complies with all three objectives of Goal 2 of the NIDCR Strategic Plan. Dr. Uskokovic will conduct a study which will involve: a) Synthesis of multifunctional therapeutic and osteoinductive calcium phosphate nanoparticles by means of co-precipitation from ultrasonically agitated emulsions; b) Drug release studies in bacterial and cell cultures. With respect to the proposed hypothesis, the central aim of this study is to derive fundamental correlations between the dissolution rate and drug delivery efficiency of calcium phosphate based carriers and their chemical composition and microstructure. This K99/R00 award is conceived to lead to an R01 grant, the writing of which will begin before the end of the award period.

此K99/R 00指导研究职业发展奖申请正在提交给 国家牙科和颅面研究所（NIDCR）。候选人武克博士 Uskokovic是一名物理化学家和纳米技术专家，他寻求获得更多的培训， 细胞培养实验和其他生物学分析方法领域 奖他的目标是发展多功能和纳米结构领域的研究技能， 用于硬组织修复的生物材料。这项提案的目标将提高博士。 Uskokovic的智能生物材料制造知识，用于同时时间控制 药物释放和患病硬组织的再生。建议是钙的合成 磷酸盐/聚合物复合颗粒，其包封克林霉素，克林霉素是一种用于 治疗骨髓炎，以及测试抗微生物特性和骨诱导 体外给定材料的性能。这项研究的中心假设是， 可以使用磷酸钙的化学计量和粒度来调节磷酸钙的动力学。 释放包封的药物。乌斯科科维奇博士将进行他的培训和研究活动 在一个专家指导团队的指导下， 马歇尔、斯特凡·哈伯利兹、吴莉和彼得·鲁默。他将获得更多的咨询， Drs.毛罗·法拉利安东尼·托姆西亚和查尔斯·胡佛。鉴于生物工程和 口腔和颅面组织再生的生物材料研究方法已经 被NIDCR选为优先目标之一（目标I-5，目标1，NIDCR战略 计划2009-2013年），目前的应用程序的目标将解决高度实际的口腔，牙科和 颅面健康问题通过这条通往独立的道路训练Uskokovic博士 该奖项是基于他在材料科学和工程方面的专业知识以及UCSF的支持 牙科学校是过去15年来NIH资助最多的一所学校，也符合所有三项要求。 实现了NIDCR战略计划的目标2。乌斯科科维奇博士将进行一项研究， 涉及：a）多功能治疗性和骨诱导性磷酸钙的合成 通过从超声搅拌的乳液中共沉淀的纳米颗粒; B）药物 在细菌和细胞培养物中的释放研究。对于提出的假设， 本研究的主要目的是推导出溶出速率与 基于磷酸钙的载体的药物递送效率及其化学组成， 微观结构这个K99/R 00奖被认为是导致R 01赠款，其写作， 将在奖励期结束前开始。