Na/K-ATPase Reduction in Renal Disease-Related Cardiac Dysfunction

肾病相关心脏功能障碍中 Na/K-ATP 酶的减少

• 批准号: 8578103
• 负责人: Jiang Tian
• 金额: $ 36.7万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-15 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8578103
• 关键词: Abbreviations; Age; Alleles; Angiotensin II Receptor; Angiotensin-Converting Enzyme Inhibitors; Animal Model; Animals; Antibodies; Apoptosis; Attenuated; Blood Pressure; Blood specimen; Brain natriuretic peptide; Cardiac; Cardiac Glycosides; Cardiomyopathies; Cardiovascular system; Cell Death; Cell Survival; Cells; Cessation of life; Chronic Kidney Failure; Complex; Congestive Heart Failure; Corticotropin; Couples; Data; Deterioration; Digibind; Dilated Cardiomyopathy; Functional disorder; Gender; Genes; Genetic; Goals; Growth; Heart; Human; In Vitro; Kidney; Kidney Diseases; Kidney Failure; Lead; Left ventricular structure; Link; Measures; Membrane; Methods; Mus; Muscle Cells; Na(+)-K(+)-Exchanging ATPase; Nephrectomy; Neutralization Tests; Operative Surgical Procedures; Outcome; Pathway interactions; Patients; Peptides; Pharmaceutical Preparations; Process; Randomized Controlled Clinical Trials; Receptor Protein-Tyrosine Kinases; Renal Artery Stenosis; Renal function; Renin-Angiotensin System; Role; Signal Pathway; Signal Transduction; Testing; Time; Tissues; Transgenic Mice; Troponin I; United States National Institutes of Health; Validation; Work; adjudicate; attenuation; heart function; human study; in vivo; marinobufagenin; mouse model; new therapeutic target; pro-apoptotic protein; protein expression; renal ischemia; research study; response; tool

DESCRIPTION (provided by applicant): Chronic renal disease (CKD) is closely related with poor cardiovascular outcomes. Our recent experimental data indicate that Na/K-ATPase reduction may be related with renal insufficiency-induced cardiac dysfunction. First, we have observed that 5/6th partial nephrectomy (PNx) induces a time-dependent decrease of Na/K-ATPase in the mouse heart along with maladaptive cardiac remodeling and deterioration in heart function. The left ventricle initially shows hypertrophic growth and then dilation, which correlates with changes in Na/K-ATPase expression. Second, we have new evidence demonstrating that reduction in Na/K-ATPase potentiates cardiotonic steroid (CTS)-induced cardiac cell death in vitro, and dilated cardiomyopathy in vivo. In transgenic mice, genetic reduction of Na/K-ATPase stimulates the expression of pro-apoptotic proteins and potentiates CTS-induced cardiac cell death. It also causes decreased contractile function in these mice. Third, our data have revealed that in normal cardiac cells there exists a self-protection mechanism preserving the membrane abundance of Na/K-ATPase and protecting cells from death. Reduction of Na/K-ATPase attenuates the signaling function that is related with this self-protection. Furthermore, our work demonstrates that endogenous CTS are elevated in animals and humans with renal diseases. These studies lead us to hypothesize that reduction of Na/K-ATPase together with a sustained increase in CTS potentiates myocyte apoptosis and results in cardiac dysfunctions in renal insufficiency. To test this hypothesis we will conduct experiments in a well established PNx animal model, and use transgenic mice and newly developed tools to assess the pathways that are related with myocyte death. In parallel, we will perform the human study to link renal function and CTS release to cardiac dysfunction.

描述（由申请人提供）：慢性肾脏疾病（CKD）与不良心血管预后密切相关。我们最近的实验数据表明，Na/ k - atp酶降低可能与肾功能不全引起的心功能障碍有关。首先，我们观察到5/6肾部分切除术（PNx）诱导小鼠心脏中Na/ k - atp酶的时间依赖性降低，同时伴有心脏重构不良和心功能恶化。左心室最初表现为肥厚生长，然后扩张，这与Na/ k - atp酶表达的变化有关。其次，我们有新的证据表明，Na/ k - atp酶的减少在体外增强了强心性类固醇（CTS）诱导的心肌细胞死亡和体内扩张性心肌病。在转基因小鼠中，Na/ k - atp酶的遗传减少刺激促凋亡蛋白的表达，并增强cts诱导的心脏细胞死亡。它还会导致这些小鼠的收缩功能下降。第三，我们的数据表明，在正常的心脏细胞中存在一种自我保护机制，保持Na/ k - atp酶的膜丰度，保护细胞免于死亡。Na/ k - atp酶的减少减弱了与这种自我保护相关的信号功能。此外，我们的工作表明内源性CTS在患有肾脏疾病的动物和人类中升高。这些研究使我们假设Na/ k - atp酶的减少与CTS的持续增加一起增强了心肌细胞凋亡并导致肾功能不全的心功能障碍。为了验证这一假设，我们将在一个完善的PNx动物模型中进行实验，并使用转基因小鼠和新开发的工具来评估与肌细胞死亡相关的途径。同时，我们将进行人体研究，将肾功能和CTS释放与心功能障碍联系起来。