Analysis of Novel Acinetobacter baumannii Adhesins

新型鲍曼不动杆菌粘附素的分析

• 批准号: 8549948
• 负责人: Anthony A Campagnari
• 金额: $ 18.62万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-26 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8549948
• 关键词: Accounting; Acinetobacter baumannii; Antibiotic Resistance; Bacillus (bacterium); Bacteremia; Bacteria; Bacterial Adhesins; Communities; Desiccation; Development; Devices; Disease Outbreaks; Disinfectants; Employee Strikes; Environment; Goals; Health care facility; Healthcare; Human; Immunocompromised Host; In Vitro; Incidence; Indwelling Catheter; Infection; Infection prevention; Investigation; Knowledge; Lead; Medical; Methods; Microbial Biofilms; Nosocomial Infections; Organism; Pathogenesis; Patients; Pneumonia; Reporting; Research; Resistance; Role; Staging; Structure; Surface; Testing; Time; Urinary tract infection; antimicrobial; design; in vivo; intercellular connection; novel; pathogen; prevent; response

DESCRIPTION (provided by applicant: Acinetobacter baumannii is a Gram-negative bacillus best known for causing health-care associated infections. Over the past decade infections with A. baumannii have increased significantly accounting for approximately 1-3% of all hospital-acquired infections usually involving immunocompromised hosts. A.baumannii is also a leading cause of device-associated infections such as pneumonia in intubated patients and urinary tract infections in patients with indwelling catheters. There have been occasional reports implicating A. baumannii as an uncommon cause of severe community-acquired pneumonia and bacteremia although these have been fairly localized. Perhaps more disturbing than the increase in A.baumannii incidence is the rapid emergence of antibiotic resistant isolates reported in numerous facilities around the globe. This is particularly troubling because A. baumannii has demonstrated a striking ability to survive and persist on abiotic surfaces for extended periods of time. Despite numerous efforts that focus on A. baumannii antibiotic resistance there are surprisingly few research efforts designed to identify mechanisms of persistence and essential steps of pathogenesis. Thus there is a significant gap in our knowledge of the basic mechanisms utilized by A. baumannii to persist on abiotic surfaces and to colonize and infect the host. The first goal of this application is to identify and characterize novel adhesins expressed by A. baumannii in response to contact with abiotic surfaces, to define the role of these structures in biofilm formation and determine if these surface structures represent attractive targets for eradication of environmental reservoirs. The second goal of this application is to determine the role of these surface structures in A. baumannii pathogenesis in vivo. We will test out hypotheses in the following specific aims: Specific Aim 1: Characterize the factors that promote attachment to abiotic surfaces, intercellular connections and biofilm formation in vitro. Specific Aim 2. Define the role of novel adhesin(s) to A. baumannii persistence and survival in vivo.

DESCRIPTION (provided by applicant: Acinetobacter baumannii is a Gram-negative bacillus best known for causing health-care associated infections. Over the past decade infections with A. baumannii have increased significantly accounting for approximately 1-3% of all hospital-acquired infections usually involving immunocompromised hosts. A.baumannii is also a leading cause of device-associated infections such as插管患者的肺炎和尿路感染偶尔有报道，这意味着鲍曼尼a。不常见的肺炎和细菌的罕见原因，尽管这些都在围绕a.baumannii interib a.baumanni risticities reveneration特别令人不安的是，鲍曼尼曲杆菌（A.非生物表面并殖民和感染宿主的第一个目标是识别和表征 鲍曼曼曲霉在与非生物表面接触的响应中表达的新型粘合剂，以定义这些结构在生物膜形成中的作用，并确定这些表面结构是否代表了消除环境储层的有吸引力的目标。该应用的第二个目标是确定这些表面结构在体内的鲍曼尼发病机理中的作用。我们将在以下特定目的中检验假设：具体目的1：表征促进对非生物表面，细胞间连接和生物膜形成的因素。具体目的2。定义了新型粘附素对鲍曼尼的持久性和体内生存的作用。