Light Adaptation and Circadian Modulation

光适应和昼夜节律调节

• 批准号: 8910742
• 负责人: Gregory Darin Field
• 金额: $ 38.96万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8910742
• 关键词: Area; Biological Assay; Brain; Cell physiology; Circadian Rhythms; Cone; Coupling; Data; Development; Dopamine; Environment; Exhibits; Gap Junctions; Goals; Health; Knowledge; Light; Light Adaptations; Lighting; Measures; Mediating; Melatonin; Morphology; Mus; Nature; Neural Retina; Outcome; Output; Paracrine Communication; Pathway interactions; Phase; Photoreceptors; Population; Process; Property; Public Health; Pupil light reflex; Research; Retina; Retinal; Retinal Degeneration; Retinal Diseases; Retinal Ganglion Cells; Signal Transduction; Signaling Molecule; Stem cells; System; Techniques; Technology; Testing; Vertebrate Photoreceptors; Vision; Visual; Work; cell type; coping; extrastriate visual cortex; gene therapy; innovation; multi-electrode arrays; parallel processing; photoreceptor disc; programs; receptive field; relating to nervous system; response; retinal prosthesis; retinal rods; signal processing; visual information; visual process; visual processing; visual stimulus

DESCRIPTION (provided by applicant): There is a fundamental gap in understanding how the parallel processing of visual information performed by the retina is modified by light adaptation and the circadian cycle. The existence of this gap precludes an understanding of how visual scenes are encoded by the retina, and decoded by the brain, across the diverse visual environments encountered from night to day. The objective here is to identify how light adaptation with the circadian cycle alters retinal ganglion cell (RGC) function. RGCs consist of ~20 distinct types. Each type carries different information about the visual scene to the brain. Cumulatively, the RGCs send this information to ~25 different brain areas. To cope with the diverse lighting conditions of natural environments, light adaptation and the circadian cycle dovetail to dynamically modulate retinal function. Dopamine and melatonin are two key signaling molecules in this process. Yet, their net impact on modulating visual signals across diverse RGC types remains elusive. The central hypothesis is that light adaptation, bolstered by the circadian cycle, exerts different changes in different RGC types. To test this hypothesis, this proposal has three specific aims: (1) determine the impact of light adaptation on response properties in many RGC types; (2) determine the impact of circadian cycle on response properties in many RGC types; and (3) determine the impact of two key circadian signals, dopamine and melatonin, on RGC function. Electrophysiological recording will be made from hundreds of RGCs simultaneously using a large-scale multielectrode array. Diverse visual stimuli will be presented to the isolated retina while recording from the RGCs to determine their light response properties. These response properties will be measured at different light levels and during different phases of the circadian cycle. Mouse lines with disrupted dopamine and/or melatonin signaling, will be used to understand how these molecules alter RGC responses under diverse lighting conditions. The proposed research is innovative because it utilizes a recently developed large-scale parallel neural recording technology to determine the interplay between parallel processing, light adaptation and the circadian cycle. The proposed research is significant because it will provide major advances in our understanding of how neural populations in the retina adapt to changes in light level, and how this adaptation is modulated by the circadian cycle. Further, these data will provide strong constraints in three areas: (1) how cellular and circuit mechanisms in the retina contribute to light adaptation and circadian modulation of visual signaling; (2) how central visual areas process retinal signals across light levels between night and day; and (3) the development of computational and theoretical principles for describing and explaining the functional impact of light adaptation. Ultimately this research will unify our understanding of the two most central functions of the neural retina: establishing the parallel processing of visual information and adapting to diverse visual environments.

描述（由申请人提供）：在理解视网膜对视觉信息的并行处理是如何被光适应和昼夜周期所改变的方面，存在一个根本性的空白。这一差距的存在阻碍了我们对视觉场景如何在昼夜不同的视觉环境中由视网膜编码和由大脑解码的理解。这里的目的是确定与昼夜周期的光适应如何改变视网膜神经节细胞（RGC）功能。rgc由大约20种不同的类型组成。每种类型将不同的视觉场景信息传递给大脑。RGCs累积起来将这些信息发送到大约25个不同的大脑区域。为了应对自然环境中不同的光照条件，光适应和昼夜周期相吻合，动态调节视网膜功能。多巴胺和褪黑激素是这一过程中的两个关键信号分子。然而，它们对不同RGC类型的视觉信号调制的净影响仍然难以捉摸。核心假设是，在昼夜周期的支持下，光适应在不同的RGC类型中产生不同的变化。为了验证这一假设，本提案有三个具体目标：(1)确定光适应对许多RGC类型的响应特性的影响；(2)确定昼夜周期对多种研究资助机构响应特性的影响；(3)确定多巴胺和褪黑激素这两个关键昼夜信号对RGC功能的影响。电生理记录将使用大规模多电极阵列同时从数百个rgc进行。不同的视觉刺激将呈现在孤立的视网膜上，同时从RGCs记录以确定其光响应特性。这些响应特性将在不同的光照水平和昼夜周期的不同阶段进行测量。多巴胺和/或褪黑激素信号被破坏的小鼠系将被用来了解这些分子如何在不同的光照条件下改变RGC的反应。这项研究具有创新性，因为它利用了最近开发的大规模并行神经记录技术来确定并行处理、光适应和昼夜周期之间的相互作用。这项提议的研究意义重大，因为它将为我们理解视网膜中的神经群如何适应光照水平的变化，以及这种适应是如何被昼夜周期调节的，提供重大进展。此外，这些数据将在三个领域提供强有力的约束：(1)视网膜中的细胞和电路机制如何促进光适应和视觉信号的昼夜节律调节；(2)中央视觉区如何处理昼夜不同光照水平的视网膜信号；(3)描述和解释光适应的功能影响的计算和理论原理的发展。最终这