Stress-induced hypertension and the role of the neuroimmune system

压力诱发的高血压和神经免疫系统的作用

• 批准号: 8803802
• 负责人: Paul J Marvar
• 金额: $ 24.87万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-15 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8803802
• 关键词: Address; Adrenal Glands; Adult; Affect; American; Amygdaloid structure; Angiotensin II; Angiotensin Type 1a Receptor; Angiotensinogen; Angiotensins; Atherosclerosis; Behavioral; Blood - brain barrier anatomy; Blood Pressure; Brain; Brain Stem; Cardiovascular Diseases; Cardiovascular system; Catecholamines; Cell Nucleus; Cells; Chronic; Communication; Corticotropin-Releasing Hormone; DOCA; Data; Development; Disease; Disease Progression; Epidemiology; Functional disorder; Genes; Heart Rate; Homeostasis; Hormones; Hypertension; Hypothalamic structure; Immune Cell Activation; Immune System Diseases; Immune System and Related Disorders; Immune system; Inflammation; Inflammatory; Laboratories; Lesion; Life; Link; Liquid substance; Lymphocyte Activation; Maintenance; Mediating; Mental Depression; Midbrain structure; Mus; Neuraxis; Neuroimmunomodulation; Neurons; Neuropeptides; Neurosecretory Systems; Norepinephrine; Nucleus solitarius; Organ; Pathogenesis; Peptides; Peripheral; Physiological; Pituitary Gland; Plasma; Play; Positioning Attribute; Production; Prosencephalon; Psychological Stress; Research; Risk; Role; Signal Pathway; Signal Transduction; Site; Societies; Stimulus; Stress; Stroke; Structure; Structure of terminal stria nuclei of preoptic region; Subfornical Organ; System; T-Cell Activation; T-Lymphocyte; Technology; Testing; Third ventricle structure; Tissues; Transgenic Organisms; Viral Vector; adaptive immunity; biological adaptation to stress; blood pressure regulation; disability; interdisciplinary approach; neural circuit; neuroimmunology; neurotransmission; novel; organum vasculosum of the lamina terminalis; paraventricular nucleus; relating to nervous system; response; salt sensitive hypertension; translational neuroscience; vascular inflammation

It has been become increasingly clear that T lymphocytes play an important role in the pathophysiology of hypertension and more recently in psychological stress and depression related disabilities. Given that chronic psychological stress is also a key contributing factor to the development and maintenance of hypertension, further understanding of the adaptive immune response and the underlying neuroimmune mechanism(s) is warranted. The central nervous system (CNS) plays an essential role in the regulation of blood pressure and has long been known to have bi-directional communication with the immune system. Recently our group has shown that the CNS, in particular the anteroventral third ventricle (AV3V) forebrain region, is critical for the development of peripheral vascular inflammation and T cell activation during angiotensin II-induced hypertension. The results of these studies reveal a new understanding for the link between central signals, peripheral inflammation and hypertension. In the proposed studies we plan to investigate the role of the adaptive immune response in stress-induced hypertension and to further characterize the underlying neurocircuitry. The bed nucleus of the stria terminalis (BNST) is a rostral forebrain structure that is uniquely positioned to integrate stress information and also has neurons that project to the sites involved in blood pressure regulation such as the AV3V region. Stimulation of the BNST mimics stress-induced neuroendocrine and autonomic responses, i.e. elevation of blood pressure, increased catecholamines and corticotropin-releasing hormone (CRH) release. In the proposed studies, we will investigate the role of BNST and CRH in blood pressure regulation and peripheral vascular inflammation in a setting of stress-induced hypertension. Using Cre-lox technology, we plan to use genetically modified mice that will allow us to specifically identify CRH producing cells in the BNST as well as the ability to delete genes such as angiotensinogen in CRH producing neurons. We hypothesize that increased CRH release within the BNST is required for stress-induced hypertension and that this CRH- and BNST involvement in hypertension is dependent on increased T lymphocyte activation and peripheral vascular inflammation. These studies will provide new information for the central and peripheral mechanisms that mediate inflammatory diseases such as hypertension and may provide a better understanding for the link between the negative impact of stress on cardiovascular disease development. The combined expertise in the pathophysiology of hypertension, stress, translational neuroscience and neuroimmunology afford us a unique opportunity to pursue this research.

越来越清楚的是，T淋巴细胞在人类免疫缺陷中发挥着重要作用。 高血压的病理生理学以及最近在心理应激和抑郁方面的研究 相关的残疾。鉴于慢性心理压力也是导致 高血压的发展和维持，加深对适应性的认识 免疫反应和潜在的神经免疫机制(S)是有根据的。中环 神经系统(CNS)在血压调节中起着至关重要的作用，长期以来 已知与免疫系统有双向通信。最近我们这个小组 研究表明，中枢神经系统，尤其是前腹侧第三脑室(AV3V)前脑区， 在外周血管炎症和T细胞活化过程中起关键作用 血管紧张素II诱导的高血压。这些研究的结果揭示了一种新的理解 中枢信号、外周炎症和高血压之间的联系。在建议的 我们计划研究适应性免疫反应在应激诱导中的作用 高血压，并进一步表征潜在的神经回路。床上的核心 终纹(BNST)是一种吻侧前脑结构，具有整合的独特位置 压力信息，也有神经元投射到参与血压的部位 监管如AV3V区域。刺激BNST模拟应激诱导 神经内分泌和自主神经反应，即血压升高 儿茶酚胺和促肾上腺皮质激素释放激素(CRH)的释放。在拟议的研究中， 我们将探讨BNST和CRH在血压调节和外周调节中的作用 应激性高血压环境中的血管炎症。使用CRE-LOX技术，我们 计划使用转基因小鼠，使我们能够专门识别产生CRH的 BNST中的细胞以及删除CRH中的血管紧张素原等基因的能力 产生神经元。我们假设需要在BNST内增加CRH释放 对于应激性高血压，CRH和BNST在高血压中的参与 依赖于T淋巴细胞活化增加和外周血管炎症。这些 研究将为中枢和外周调节机制提供新的信息 炎症性疾病，如高血压，可能会提供更好的了解 在压力对心血管疾病发展的负面影响之间。合并后的 在高血压、应激、翻译神经科学和 神经免疫学为我们提供了一个进行这项研究的独特机会。