Photothermal Nanocomposites for Tissue Repair

用于组织修复的光热纳米复合材料

• 批准号: 8945323
• 负责人: Kaushal Rege
• 金额: $ 38.16万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-09 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8945323
• 关键词: American; Anastomosis - action; Bacteria; Biochemical; Biocompatible; Biocompatible Materials; Clinical; Collagen; Colon; Colorectal; Colorectal Cancer; Colorectal Surgery; Deposition; Development; Drug Delivery Systems; Drug usage; Elastin; Encapsulated; Evaluation; Excision; Extravasation; Family suidae; Fibrin; Generations; Goals; Gold; Heating; Human; Immune response; Inflammatory Bowel Diseases; Intestines; Laser injury; Lasers; Lead; Light; Liquid substance; Manuscripts; Matrix Metalloproteinase Inhibitor; Measurement; Mechanics; Mediating; Medicine; Methods; Modeling; Morbidity - disease rate; Operative Surgical Procedures; Pharmaceutical Preparations; Physiologic pulse; Prevention; Procedures; Property; Proteins; Recovery; Reporting; Research; Rupture; Surgical Anastomosis; Surgical incisions; Surgical sutures; Tensile Strength; Time; Tissue Model; Tissues; Translations; United States; Welding; base; biomacromolecule; biomaterial compatibility; clinical application; gastrointestinal; improved; in vivo; irradiation; mortality; mouse model; nanocomposite; nanorod; news; novel; polypeptide; pressure; prevent; public health relevance; repaired; response; seal; small molecule; tissue repair; tissue welding

 DESCRIPTION: The ability of photothermal biomaterials to convert light to heat can have diverse applications in medicine. However, the lack of effective biomaterials has adversely impacted progress and clinical translation in this promising field. The overall goal of this proposed research is to generate, characterize, and apply photothermal nanocomposites for effective tissue repair. In this approach, we will generate nanocomposites in which, gold nanorods are incorporated within biocompatible biomacromolecules including, elastin-like polypeptides (ELPs), collagen, fibrin and their blends. The photothermal response of these nanocomposites will be investigated using both, pulsed and continuous wavelength lasers at different power intensities. In addition, the mechanical properties and efficacy of laser-triggered drug (MMP inhibitor) delivery from these nanocomposites will be determined (Specific Aim 1). Nanocomposites with effective photothermal properties will be investigated for their ability to repair ruptured porcine intestinal tissue ex vivo; an incision model of tissue rupture will be investigated. Both pulsed and continuous lasers, at different power intensities, will be investigated in order to facilitate nanocomposite-mediated localized photothermal welding of the ruptured tissue. Tensile strength, leak pressure and burst pressure will be investigated in order to determine the mechanical integrity of the nanocomposite-repaired tissue. The efficacy of photothermal nanocomposites for preventing bacterial leakage from bacteria-rich intestinal lumen will also be investigated. Nanocomposite composition and operating conditions that result in the most effective recovery of tissue properties compared to intact tissue will be identified (Specific Aim 2). The most effective nanocomposite type and operating conditions will be further evaluated for maintaining tissue integrity and facilitating repair in a mouse model of colon leakage. Mechanical properties, including burst pressures, biochemical and immune responses, effect of MMP inhibitor delivery, and photothermal effects will be determined following in vivo welding. Longer-term survival studies will also be carried out (Specific Aim 3). It is anticipated that the current research will lead to transformative developments in both, fundamental studies as well as application of biocompatible photothermal nanocomposites for tissue repair. Findings from this research have very high potential for direct translation to several clinical applications that can benefit from the several advantages of photothermal tissue repair.

 描述：光热生物材料将光转化为热的能力在医学上有多种应用。然而，缺乏有效的生物材料对这一有前途的领域的进展和临床转化产生了不利影响。这项研究的总体目标是产生，表征和应用光热纳米复合材料进行有效的组织修复。在这种方法中，我们将产生纳米复合材料，其中，金纳米棒被纳入生物相容性生物大分子，包括弹性蛋白样多肽（ELP），胶原蛋白，纤维蛋白和它们的混合物。这些纳米复合材料的光热响应将使用脉冲和连续波长激光在不同的功率强度进行研究。此外，还研究了激光触发复合材料的力学性能和功效， 将确定这些纳米复合物的药物（MMP抑制剂）递送（具体目标1）。将研究具有有效光热性能的纳米复合材料在体外修复破裂猪肠组织的能力;将研究组织破裂的切口模型。脉冲和连续激光，在不同的功率强度，将进行研究，以促进纳米复合材料介导的局部光热焊接的破裂组织。将研究拉伸强度、泄漏压力和爆破压力，以确定纳米复合材料修复组织的机械完整性。还将研究光热纳米复合材料用于防止细菌从富含细菌的肠腔渗漏的功效。与完整组织相比，将确定导致组织特性最有效恢复的纳米复合材料组成和操作条件（具体目标2）。将进一步评价最有效的纳米复合材料类型和操作条件，以维持结肠渗漏小鼠模型中的组织完整性并促进修复。在体内焊接后，将确定机械性能，包括爆破压力、生化和免疫反应、MMP抑制剂递送的影响以及光热效应。还将进行长期生存研究（具体目标3）。预计目前的研究将导致基础研究以及生物相容性光热纳米复合材料用于组织修复的应用的变革性发展。这项研究的发现具有很大的潜力，可以直接转化为多种临床应用 可以从光热组织修复的几个优点中受益。